CAJ | 학술논문

目的探讨辅助性T细胞(Th1)因子在胃癌组织中的表达与胃癌患者预后的相关性及其临床意义.方法回顾性分析2006年6月至2008年1月间复旦大学附属中山医院收治的58例胃癌患者的临床资料,运用实时定量PCR技术检测58例胃癌组织中Th1细胞因子TRAV10、IRF1、TBX21、CD3Z、GZMB、GNLY、GATA3和IFNG 8种Th1细胞的mRNA表达水平,并分析其与58例胃癌患者术后生存的关系.结果 58例胃癌患者中位随访期为42.5( 1～64)个月,术后1年和3年生存率为84.5％和72.4％.单因素Cox回归分析显示,淋巴结转移、远处转移、TNM分期、脉管癌栓、GNLY mRNA表达水平及Th1细胞8因子综合表达水平与胃癌术后生存有关(均P＜0.05).GNLY的mRNA表达水平上调者和下调者3年生存率分别86.2％和58.6％,Th1细胞8因子任意上调和全部下调者3年生存率分别为79.6％和33.3％,差异均有统计学意义(均P＜0.05).多因素Cox回归分析显示,淋巴结转移和Th1细胞8因子综合表达水平是本组胃癌患者的独立预后因素(均P＜0.05).结论在Th1细胞因子中,GNLY mRNA表达水平与胃癌患者预后有关,但并不是独立因素.Th1细胞8因子综合表达水平可作为胃癌患者的独立预后因素.
목적 탐토보조성T세포(Th1)인자재위암조직중적표체여위암환자예후적상관성급기림상의의.방법 회고성분석2006년6월지2008년1월간복단대학부속중산의원수치적58례위암환자적림상자료,운용실시정량PCR기술검측58례위암조직중Th1세포인자TRAV10、IRF1、TBX21、CD3Z、GZMB、GNLY、GATA3화IFNG 8충Th1세포적mRNA표체수평,병분석기여58례위암환자술후생존적관계.결과 58례위암환자중위수방기위42.5( 1～64)개월,술후1년화3년생존솔위84.5％화72.4％.단인소Cox회귀분석현시,림파결전이、원처전이、TNM분기、맥관암전、GNLY mRNA표체수평급Th1세포8인자종합표체수평여위암술후생존유관(균P＜0.05).GNLY적mRNA표체수평상조자화하조자3년생존솔분별86.2％화58.6％,Th1세포8인자임의상조화전부하조자3년생존솔분별위79.6％화33.3％,차이균유통계학의의(균P＜0.05).다인소Cox회귀분석현시,림파결전이화Th1세포8인자종합표체수평시본조위암환자적독립예후인소(균P＜0.05).결론 재Th1세포인자중,GNLY mRNA표체수평여위암환자예후유관,단병불시독립인소.Th1세포8인자종합표체수평가작위위암환자적독립예후인소.
Objective To investigate the association of the expressions of Th1 cytokines in gastric cancer tissue and the prognosis of patients with gastric cancer after radical resection.Methods Fifty-eight patients with gastric cancer treated at the Zhongshan Hospital of Fudan University were retrospectively analyzed.The expressions of Th1 cytokines mRNA were detected in tumor tissues by real-time polymerase chain reaction (RT-PCR) method in Th1 cells including TRAV10,IRF1,TBX21,CD3Z,GZMB,GATA3,and IFNG.Association of Th1 cytokines mRNA expressions and prognosis was evaluated.Results The median follow up was 42.5( 1-64) months.The 1-year survival rate was 84.5％ and the 3-year survival rate was 72.4％.Univariate Cox regression analysis showed that lymph node metastasis,distant metastasis,TNM staging,lymphovascular invasion,GNLY mRNA expression,and the overall expression level of the 8 types of Th1 cells were associated with the prognosis of patients with gastric cancer(all P＜0.05 ).The 3-year survival was 86.2％ in patients with increased expression of mRNA and 58.6％ in those with decreased expression.The 3-year survival was 79.6％ in patients with any increase in the 8 Th1 cytokines and 33.3％ in those with consistent downregulation of the 8 cytokines.Mutlivariate Cox analysis showed that lymph node metastasis and the overall expression level of 8 Th1 cytokines were independent risk factors associated with the prognosis of patients with gastric cancer in this cohort (both P＜0.05).Conclusions The mRNA expression of GNLY is associated with the prognosis of patients with gastric cancer but is not an independent risk factor.The combination of mRNA expressions of the eight cytokines is an independent prognostic factor.