CAJ | 학술논문

在GT1-7细胞用实时定量PCR检测腺苷酸活化的蛋白激酶(AMPK)对KiSS-1mRNA水平的影响,以报告基因技术检测KiSS-1基因启动子的活性,用Western印迹法检测AMPK对转录因子SP1蛋白表达及其在亚细胞中分布的影响.结果显示,AMPK能降低GT1-7细胞KiSS-1mRNA的表达水平,并能抑制KiSS-1基因的启动子活性；SP1能够增强KiSS-1基因的启动子活性；AMPK能够抑制SP1向细胞核的转位.提示AMPK可能通过抑制转录因子SP1的转位而降低KiSS-1基因的表达.
재GT1-7세포용실시정량PCR검측선감산활화적단백격매(AMPK)대KiSS-1mRNA수평적영향,이보고기인기술검측KiSS-1기인계동자적활성,용Western인적법검측AMPK대전록인자SP1단백표체급기재아세포중분포적영향.결과현시,AMPK능강저GT1-7세포KiSS-1mRNA적표체수평,병능억제KiSS-1기인적계동자활성；SP1능구증강KiSS-1기인적계동자활성；AMPK능구억제SP1향세포핵적전위.제시AMPK가능통과억제전록인자SP1적전위이강저KiSS-1기인적표체.
The effect of AMP-activated protein kinase (AMPK) on KiSS-1 mRNA levels was detected by realtime PCR in the hypothalamic GT1-7 neurons. The promoter activity of KiSS-1 gene was detected by DualLuciferase Reporter Assay System.The effects of AMPK on the protein expression and subcellular distribution of SP1 were determined by Western blot.The results showed that AMPK reduced the mRNA expression and promoter activity of KiSS-1 gene while SP1 increased the promoter activity of KiSS-1 gene. Besides,AMPK alse decreased the translocation of SP1.These results suggest that AMPK may inhibit the expression of KiSS-1 gene by decreasing the translocation of SP1 from cytoplasm to nucleus in the hypothalamus GT1-7 neurons.