CAJ | 학술논문

目的建立稳定的小鼠左肺移植模型.方法取近交系雄性Balb/c小鼠40只作为肺移植的供、受者,每对供、受者间大小和体重基本匹配,采用三套管法进行原位左肺移植.术后1个月,取受鼠进行胸部CT检查,取阻断右肺门前、后的移植左肺静脉血进行血液气体分析,取移植肺组织进行病理学检查.结果实验共行肺移植20例,除1例因肺静脉撕裂导致手术失败外,其余19例均成功进行手术,手术成功率为95％(19/20).成功模型的套管吻合均一次成功,术后1例受鼠因发生感染而死亡,死亡率为5.2％(1/19).套管准备时间为(2.0±0.5)min,供肺切取时间为(5.0±1.3) min,供肺冷缺血时间为(35.6±5.9)min,热缺血时间为(25.3±7.2)min,供、受者血管套管耗时(21.0±5.6)min,受者手术耗时(30.0±4.2)min,建立模型总耗时(85±15) min.术后1个月,显微CT检查结果显示,受鼠移植肺野清晰,支气管套管通畅；血液气体分析结果显示,阻断前、后的氧气分压分别为(106.9±5.8) mm Hg(1mm Hg=0.133 kPa)和(105.0±8.7) mm Hg,二者比较,差异无统计学意义(P＞0.05)；移植肺组织的病理学检查显示,移植肺结构正常,肺间隔正常,肺泡通气良好.结论采用三套管法建立的小鼠肺移植模型具有操作简便易行,支气管吻合口径更大,手术成功率高,以及模型稳定等特点,这对于推动肺移植的基础实验研究有重要意义.
목적 건립은정적소서좌폐이식모형.방법 취근교계웅성Balb/c소서40지작위폐이식적공、수자,매대공、수자간대소화체중기본필배,채용삼투관법진행원위좌폐이식.술후1개월,취수서진행흉부CT검사,취조단우폐문전、후적이식좌폐정맥혈진행혈액기체분석,취이식폐조직진행병이학검사.결과 실험공행폐이식20례,제1례인폐정맥시렬도치수술실패외,기여19례균성공진행수술,수술성공솔위95％(19/20).성공모형적투관문합균일차성공,술후1례수서인발생감염이사망,사망솔위5.2％(1/19).투관준비시간위(2.0±0.5)min,공폐절취시간위(5.0±1.3) min,공폐랭결혈시간위(35.6±5.9)min,열결혈시간위(25.3±7.2)min,공、수자혈관투관모시(21.0±5.6)min,수자수술모시(30.0±4.2)min,건립모형총모시(85±15) min.술후1개월,현미CT검사결과현시,수서이식폐야청석,지기관투관통창；혈액기체분석결과현시,조단전、후적양기분압분별위(106.9±5.8) mm Hg(1mm Hg=0.133 kPa)화(105.0±8.7) mm Hg,이자비교,차이무통계학의의(P＞0.05)；이식폐조직적병이학검사현시,이식폐결구정상,폐간격정상,폐포통기량호.결론 채용삼투관법건립적소서폐이식모형구유조작간편역행,지기관문합구경경대,수술성공솔고,이급모형은정등특점,저대우추동폐이식적기출실험연구유중요의의.
Objective To investigate the establishment of stable mice orthotopic left lung transplant model Methods Forty male Balb/c mice randomly served as recipients and donors.Each couple had the similar weight. Three-cuff technique was used to establish mice orthotopic lung transplant model.The micro CT of chest and the artery blood analysis were detected at first month after transplantation.Results The achievement ratio was 95％.More than 90％ of the recipients survived after surgery.Cold ischemia time was (35.6±5.9) min,warm ischemia time was (25.3±7.2) min,donor lung back up time was (21.0±5.6) min,and the whole surgery time was (85±15)min.The long-term survival time achieved more than 30 days with functional lung graft.Micro CT showed clear left lung graft field one month after surgery,and left bronchus cuff was still open.The PaO2 of the artery blood was ( 106.9±5.8 ) mmHg before clipping right lung hlium,and after clipping that was (105.0±8.7) mmHg with the difference being not significant between them (P＞0.05).Histologically,the lung graft appeared very similar to the right lung one month after surgery.The pulmonary alveoli were aerated well.Conclusion Based on our experience of the orthotopic lung transplantation model in mice,through practicing,we independently and successfully established orthotopic lung transplantation model in mice.Compared to the method established by the other country,our method is easier to perform and more stable.This will benefit the basic research related to lung transplantation.