中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2009年
4期
275-280
,共6页
窦懿%张勤%章雄%董叫云%唐佳俊%廖镇江
竇懿%張勤%章雄%董叫雲%唐佳俊%廖鎮江
두의%장근%장웅%동규운%당가준%료진강
烧伤%脓毒症%生长激素%胸腺素%炎性反应
燒傷%膿毒癥%生長激素%胸腺素%炎性反應
소상%농독증%생장격소%흉선소%염성반응
Bums%Sepsis%Growth hormone%Thymosin%Inflammatory response
目的 了解胸腺素和生长激素对烧伤及烧伤并发脓毒症大鼠炎性反应的影响.方法 将SD大鼠按完全随机法分为8组,每组8只.(1)正常对照组.(2)未烧伤脓毒症组:正常大鼠注射LPS.(3)未烧伤脓毒症胸腺素组:正常大鼠先后注射胸腺素和LPS.(4)未烧伤脓毒症生长激素组:正常大鼠先后注射重组人生长激素(rhGH)和LPS.(5)烧伤组.(6)烧伤脓毒症组:大鼠烧伤后注射LPS.(7)烧伤脓毒症胸腺素组:大鼠烧伤后依次注射胸腺素和LPS.(8)烧伤脓毒症生长激素组:大鼠烧伤后依次注射rhGH和LPS.取各组大鼠脾脏组织及外周血标本,检测脾脏淋巴细胞人白细胞DR抗原(HLA-DR)表达情况,观察脾脏炎性细胞浸润情况(计分),测定外周血单核细胞Toll样受体4(TLR4)表达情况及血清TNF-α、IL-4、IL-6、IL-10水平. 结果 (1)与正常对照组比较,未烧伤脓毒症组大鼠除血清IL-10水平明显降低之外(P<0.01),其余指标均明显增高(P<0.01).未烧伤脓毒症胸腺素组大鼠HLA-DR、TLR4表达及血清TNF-α水平与未烧伤脓毒症生长激素组相近(P>0.05);但前一组大鼠脾脏炎性细胞浸润程度为(1.50±0.76)分,IL-6、IL-4、IL-10水平分别为(0.0064±0.0012)、(0.0058±0.0024)、(0.0230±0.0021)μg/mg,与后一组大鼠[(2.87±0.04)分,(0.0083±0.0018)、(0.0102±0.0021)、(0.0310±0.0027)μg/mg]比较明显降低(P<0.01).(2)烧伤组大鼠HLA-DR、TLR4表达和脾脏炎性细胞浸润程度均明显高于正常对照组而低于烧伤脓毒症组;烧伤组TNF-α、IL-6水平较正常对照组明显增高,IL-4、IL-10明显减少(P<0.01).烧伤脓毒症生长激素组HLA-DR及IL-6水平与烧伤脓毒症胸腺素组接近(P>0.05);但与后组相比,前组大鼠脾脏炎性细胞浸润程度、TLR4表达和TNF-α水平明显降低(P<0.01),IL-4、IL-10水平明显增高(P<0.01). 结论有创伤与无创伤条件下,胸腺素与生长激素干预脓毒症过度炎性反应的效果接近;有创伤因素存在时,生长激素更能抑制该炎性反应.
目的 瞭解胸腺素和生長激素對燒傷及燒傷併髮膿毒癥大鼠炎性反應的影響.方法 將SD大鼠按完全隨機法分為8組,每組8隻.(1)正常對照組.(2)未燒傷膿毒癥組:正常大鼠註射LPS.(3)未燒傷膿毒癥胸腺素組:正常大鼠先後註射胸腺素和LPS.(4)未燒傷膿毒癥生長激素組:正常大鼠先後註射重組人生長激素(rhGH)和LPS.(5)燒傷組.(6)燒傷膿毒癥組:大鼠燒傷後註射LPS.(7)燒傷膿毒癥胸腺素組:大鼠燒傷後依次註射胸腺素和LPS.(8)燒傷膿毒癥生長激素組:大鼠燒傷後依次註射rhGH和LPS.取各組大鼠脾髒組織及外週血標本,檢測脾髒淋巴細胞人白細胞DR抗原(HLA-DR)錶達情況,觀察脾髒炎性細胞浸潤情況(計分),測定外週血單覈細胞Toll樣受體4(TLR4)錶達情況及血清TNF-α、IL-4、IL-6、IL-10水平. 結果 (1)與正常對照組比較,未燒傷膿毒癥組大鼠除血清IL-10水平明顯降低之外(P<0.01),其餘指標均明顯增高(P<0.01).未燒傷膿毒癥胸腺素組大鼠HLA-DR、TLR4錶達及血清TNF-α水平與未燒傷膿毒癥生長激素組相近(P>0.05);但前一組大鼠脾髒炎性細胞浸潤程度為(1.50±0.76)分,IL-6、IL-4、IL-10水平分彆為(0.0064±0.0012)、(0.0058±0.0024)、(0.0230±0.0021)μg/mg,與後一組大鼠[(2.87±0.04)分,(0.0083±0.0018)、(0.0102±0.0021)、(0.0310±0.0027)μg/mg]比較明顯降低(P<0.01).(2)燒傷組大鼠HLA-DR、TLR4錶達和脾髒炎性細胞浸潤程度均明顯高于正常對照組而低于燒傷膿毒癥組;燒傷組TNF-α、IL-6水平較正常對照組明顯增高,IL-4、IL-10明顯減少(P<0.01).燒傷膿毒癥生長激素組HLA-DR及IL-6水平與燒傷膿毒癥胸腺素組接近(P>0.05);但與後組相比,前組大鼠脾髒炎性細胞浸潤程度、TLR4錶達和TNF-α水平明顯降低(P<0.01),IL-4、IL-10水平明顯增高(P<0.01). 結論有創傷與無創傷條件下,胸腺素與生長激素榦預膿毒癥過度炎性反應的效果接近;有創傷因素存在時,生長激素更能抑製該炎性反應.
목적 료해흉선소화생장격소대소상급소상병발농독증대서염성반응적영향.방법 장SD대서안완전수궤법분위8조,매조8지.(1)정상대조조.(2)미소상농독증조:정상대서주사LPS.(3)미소상농독증흉선소조:정상대서선후주사흉선소화LPS.(4)미소상농독증생장격소조:정상대서선후주사중조인생장격소(rhGH)화LPS.(5)소상조.(6)소상농독증조:대서소상후주사LPS.(7)소상농독증흉선소조:대서소상후의차주사흉선소화LPS.(8)소상농독증생장격소조:대서소상후의차주사rhGH화LPS.취각조대서비장조직급외주혈표본,검측비장림파세포인백세포DR항원(HLA-DR)표체정황,관찰비장염성세포침윤정황(계분),측정외주혈단핵세포Toll양수체4(TLR4)표체정황급혈청TNF-α、IL-4、IL-6、IL-10수평. 결과 (1)여정상대조조비교,미소상농독증조대서제혈청IL-10수평명현강저지외(P<0.01),기여지표균명현증고(P<0.01).미소상농독증흉선소조대서HLA-DR、TLR4표체급혈청TNF-α수평여미소상농독증생장격소조상근(P>0.05);단전일조대서비장염성세포침윤정도위(1.50±0.76)분,IL-6、IL-4、IL-10수평분별위(0.0064±0.0012)、(0.0058±0.0024)、(0.0230±0.0021)μg/mg,여후일조대서[(2.87±0.04)분,(0.0083±0.0018)、(0.0102±0.0021)、(0.0310±0.0027)μg/mg]비교명현강저(P<0.01).(2)소상조대서HLA-DR、TLR4표체화비장염성세포침윤정도균명현고우정상대조조이저우소상농독증조;소상조TNF-α、IL-6수평교정상대조조명현증고,IL-4、IL-10명현감소(P<0.01).소상농독증생장격소조HLA-DR급IL-6수평여소상농독증흉선소조접근(P>0.05);단여후조상비,전조대서비장염성세포침윤정도、TLR4표체화TNF-α수평명현강저(P<0.01),IL-4、IL-10수평명현증고(P<0.01). 결론유창상여무창상조건하,흉선소여생장격소간예농독증과도염성반응적효과접근;유창상인소존재시,생장격소경능억제해염성반응.
Objective To investigate the effect of Thymosin and growth hormone(GH) on inflam-matory response in burn rats or burn rats with sepsis. Methods Sixty-four SD rats were randomly divided into normal control group (NC, without treatment), sepsis group (S, with injection of LPS), sepsis + Thy-mosin group (ST, with successive injection of Thymosin and LPS), sepsis + GH group [SGH, with succes-sive injection of recombinant human GH (rhGH) and LPS], burn group, burn + sepsis group (BS, with in-jection of LPS after burn), burn + sepsis + Thymosin group (BST, with successive injection of Thymosin and LPS after burn), burn + sepsis + GH (BSGH, with successive injection of rhGH and LPS after burn), with 8 rats in each group. Specimens of spleen tissues were harvested to determine HLA-DR in lymphocyte and e-valuate inflammatory cell infiltration (score). Specimens of peripheral blood were collected to determine Toll-like receptor 4 (TLR4) level in monocyte and serum level of TNF-α, IL-4, IL-6, IL-10. Results Compared with those in NC group, serum level of IL-10 in S group decreased obviously, while other indices increased obviously (P<0.01). The levels of HLA-DR and TLR4 and serum level of TNF-α were similar between SGH and ST groups (P>0.05). Compared with those in SGH group [(2.87±0.04) score, and IL-6 (0.0083±0.0018) μg/mg, IL-4 (0.0102±0.0021) μg/mg, IL-10 (0.0310±0.0027) μg/mg, re-spectively], degree of inflammatory cell infiltration (1.50±0.76) score and serum levels of IL-6, IL-4, IL-10 of rats in ST group decreased obviously (0.0064±0.0012, 0.0058±0.0024, 0.0230±0.0021 μg/mg, respectively, P<0.01). The levels of HLA-DR, TLR4 and inflammatory cell infiltration degree of spleen in B group were respectively higher than those in NC group and lower than those in BS group. Com-pared with those in NC group, serum levels of TNF-α, IL-6 in B group increased significantly, while IL-4, IL-10 showed an opposite tendency. There was no obvious difference between BST and BSGH groups in ser-um levels of HLA-DR and IL-6 (P>0.05). Compared with those in BST group, inflammatory cell infiltra-tion degree in spleen and the levels of TLR, TNF-α obviously decreased (P<0.01), while IL-4 and IL-10 levels increased in BSGH group (P<0.01). Conclusions Inhibitive effects between Thymosin and GH on extensive inflammatory reaction were similar with or without trauma, and GH has better effect as compared with Thymosin when with trauma.
