CAJ | 학술논문

目的检测细胞因子通路抑制因子3( SOCS3)在前列腺癌和前列腺增生组织中的表达,分析其表达水平与临床病理参数的关系,探讨SOCS3在前列腺癌中的意义.方法通过实时荧光定量聚合酶链反应(RT-qPCR)检测SOCS3基因在前列腺癌、前列腺增生组织中的表达,免疫组织化学技术检测前列腺癌、前列腺增生组织中SOCS3蛋白的表达,结合SOCS3免疫组织化学评分与前列腺癌患者临床病理参数进行分析.结果相对于前列腺增生组织,前列腺癌患者组织中SOCS3表达下调(P＜0.01)；49例前列腺癌组织有26例阳性,29例前列腺增生组织有22例染色阳性,SOCS3在前列腺增生组织的染色强于前列腺癌组织(P＜0.05)；SOCS3表达与前列腺癌患者血清前列腺特异性抗原(PSA)水平(P＜0.01)、Gleason评分(P＜0.05)和肿瘤TNM分期呈负相关(P＜0.01),与肿瘤转移呈正相关(P＜0.01),与年龄无明显相关(P＞0.05).结论检测SOCS3可鉴别前列腺癌和前列腺增生；在前列腺癌中SOCS3表达下调可抑制前列腺癌的进展,表达上调促进前列腺癌转移.
목적 검측세포인자통로억제인자3( SOCS3)재전렬선암화전렬선증생조직중적표체,분석기표체수평여림상병리삼수적관계,탐토SOCS3재전렬선암중적의의.방법 통과실시형광정량취합매련반응(RT-qPCR)검측SOCS3기인재전렬선암、전렬선증생조직중적표체,면역조직화학기술검측전렬선암、전렬선증생조직중SOCS3단백적표체,결합SOCS3면역조직화학평분여전렬선암환자림상병리삼수진행분석.결과 상대우전렬선증생조직,전렬선암환자조직중SOCS3표체하조(P＜0.01)；49례전렬선암조직유26례양성,29례전렬선증생조직유22례염색양성,SOCS3재전렬선증생조직적염색강우전렬선암조직(P＜0.05)；SOCS3표체여전렬선암환자혈청전렬선특이성항원(PSA)수평(P＜0.01)、Gleason평분(P＜0.05)화종류TNM분기정부상관(P＜0.01),여종류전이정정상관(P＜0.01),여년령무명현상관(P＞0.05).결론 검측SOCS3가감별전렬선암화전렬선증생；재전렬선암중SOCS3표체하조가억제전렬선암적진전,표체상조촉진전렬 선암전이.
Objective To study the expression of suppressors of cytokine signaling-3 (SOCS3) in prostate cancer (PCa) and benign prostate hyperplasia ( BPH ) tissues,analysis of its expression level with clinical pathological parameters and to explore the significance of SOCS3 in PCa.Methods Using real time fluorescent quantitative polymerase chain reaction (RT-qPCR) to detect the expression of SOCS3 in PCa and BPH tiussues,immunohistochemical (IHC) validation,the combination of SOCS3 staining score and clinical pathological parameters were analyzed.Results The expression of SOCS3 in BPH tissues was significantly higher than that in PCa tissues ( P ＜ 0.01 ),22 of 29 cases of BPH were SOCS3 staining positive,PCa tissues only 26 cases showed positive,the expression of SOCS3 in BPH tissue was significantly stronger than that of PCa tissues ( P ＜ 0.05 ) ; Combined with SOCS3 stain score and the clinicopathological parameters of the corresponding PCa patients,we found that the low expression of SOCS3 was closely related with serum prostate specific antigen (PSA) level ( P ＜ 0.01 ),Gleason score ( P ＜ 0.05 ),tumor TNM stage (P＜0.01) and metastasis (P＜0.01) but not with age (P＞0.05).Conclusion Detection of SOCS3 can identify PCa and BPH; downregulation of SOCS3 may irhibit the progression of PCa,whereas upregulation promote metastasis.