CAJ | 학술논문

目的探讨改良血管内穿刺法制作大鼠蛛网膜下腔出血(SAH)模型的制作方法,以及此模型蛛网膜下腔积血分布、吸收与神经元损伤病理特征的动态变化规律.方法 SD大鼠随机分为正常、假手术和手术组,采用改良血管内穿刺法制作SAH模型,观察各组脑组织的大体形态,以及手术组各个时间点蛛网膜下腔内血液分布情况;通过苏木素-伊红(HE)染色,观察神经组织的病理学变化.结果 3～24 h蛛网膜下腔的积血由穿刺的局部脑底面逐渐向大脑凸面蛛网膜下腔弥散;48 h第四脑室可见明显积血;大脑皮层神经元水肿随时间的增加逐渐加重,24 h达到水肿高峰,并持续到48 h;7 d时神经元水肿基本恢复.结论改良血管穿刺是制作SAH模型较为理想的方法,蛛网膜下腔内血液的吸收再分布规律及神经元的动态损伤过程为SAH模型构建的评价提供了更完备的实验数据.
목적 탐토개량혈관내천자법제작대서주망막하강출혈(SAH)모형적제작방법,이급차모형주망막하강적혈분포、흡수여신경원손상병리특정적동태변화규률.방법 SD대서수궤분위정상、가수술화수술조,채용개량혈관내천자법제작SAH모형,관찰각조뇌조직적대체형태,이급수술조각개시간점주망막하강내혈액분포정황;통과소목소-이홍(HE)염색,관찰신경조직적병이학변화.결과 3～24 h주망막하강적적혈유천자적국부뇌저면축점향대뇌철면주망막하강미산;48 h제사뇌실가견명현적혈;대뇌피층신경원수종수시간적증가축점가중,24 h체도수종고봉,병지속도48 h;7 d시신경원수종기본회복.결론 개량혈관천자시제작SAH모형교위이상적방법,주망막하강내혈액적흡수재분포규률급신경원적동태손상과정위SAH모형구건적평개제공료경완비적실험수거.
Objective To investigate the modified method of endovascular perforation model,observing dynamically the absorption and redistribution characteristics of blood in subarachnoid space and the pathological features of neuronal damage after subarachnoid hemorrhage in rats. Methods The SD rats were divided into 3 groups:normal controls,sham-operated controls and SAH groups. A modified endovascular perforation model was used to induce subarachnoid hemorrhage in rats. In each group, the brains were collected to observe the gross morphology of cerebral tissue and the distribution of blood in subarachnoid space, and also examine the pathological changes of nervous tissue by HE staining. Results The blood in subarachnoid space was diffused from pavimentum cerebri to the cerebral convexities and then spread gradually in 3-24 h. The blood clots could be obviously seen in the fourth ventricle at 48 h. The edena of neurons became serious with the prolongation of time, reached its peak at 24 h, lasted to 48 h, and returned basically to normal state at 7 day. Conclusion The modified endovascular perforation is considered to be the most suitable for establishing subarachnoid hemorrhage model. It is the absorption and redistribution characteristics of blood in subarachnoid space and the dynamic process of neuronal damage that offering the more perfect data for establishing and evaluating the subarachnoid hemorrhage model.