中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2011年
7期
439-444
,共6页
王翠翠%周春林%王慧君%李庆华%刘旭萍%宫本法%刘兵城%林冬%李巍%秘营昌%王建祥
王翠翠%週春林%王慧君%李慶華%劉旭萍%宮本法%劉兵城%林鼕%李巍%祕營昌%王建祥
왕취취%주춘림%왕혜군%리경화%류욱평%궁본법%류병성%림동%리외%비영창%왕건상
白血病,淋巴细胞,急性%免疫表型%细胞遗传学
白血病,淋巴細胞,急性%免疫錶型%細胞遺傳學
백혈병,림파세포,급성%면역표형%세포유전학
Leukemia,lymphocytic,acute%Immunophenotype%Cytogenetics
目的 研究成人急性淋巴细胞白血病(ALL)生物学特征,分析WHO 2008年ALL分类的变化.方法 应用流式细胞术、R显带常规核型分析法、RT-PCR技术对初诊成人ALL患者进行免疫学、细胞遗传学、分子生物学检测.结果 ①共412例初诊成人ALL患者,男性239例,女性173例.免疫表型可分析病例410例,B系357例,T系53例.B系的髓系抗原表达比例高于T系.髓系抗原表达与CD34表达相关.②Ph+和(或)bcr-abl+ALL(简称Ph+ ALL)患者93例,主要见于CD10+ALL,患者WBC、髓系抗原表达及CD34阳性率高于Ph- ALL患者.ALL伴MLL重排(MLL+)患者12例,主要见于早期前B-ALL.③据WHO 2008年分类标准,可分析病例299例,其中B-ALL伴重现性细胞遗传学异常126例,包括B-ALL伴t(9;22)(q34;q11.2)或bcr-abl阳性92例,MLL+ ALL 10例,超二倍体11例,亚二倍体9例,E2A-PBX+ALL 3例,TEL-AML1+ ALL 1例.各组间CD34及髓系抗原表达差异有统计学意义.B-ALL非特指型120例.将Ph+ ALL、MLL+ ALL、亚二倍体及E2A-PBX+ ALL患者作为一组,与其他患者相比,前者年龄、WBC、HGB、外周血幼稚细胞比例、乳酸脱氢酶水平均较高.结论 需结合免疫表型、细胞遗传学、分子生物学分析结果以明确ALL诊断.ALL的WHO 2008年分类根据细胞遗传学对B-ALL作了更详细分类.
目的 研究成人急性淋巴細胞白血病(ALL)生物學特徵,分析WHO 2008年ALL分類的變化.方法 應用流式細胞術、R顯帶常規覈型分析法、RT-PCR技術對初診成人ALL患者進行免疫學、細胞遺傳學、分子生物學檢測.結果 ①共412例初診成人ALL患者,男性239例,女性173例.免疫錶型可分析病例410例,B繫357例,T繫53例.B繫的髓繫抗原錶達比例高于T繫.髓繫抗原錶達與CD34錶達相關.②Ph+和(或)bcr-abl+ALL(簡稱Ph+ ALL)患者93例,主要見于CD10+ALL,患者WBC、髓繫抗原錶達及CD34暘性率高于Ph- ALL患者.ALL伴MLL重排(MLL+)患者12例,主要見于早期前B-ALL.③據WHO 2008年分類標準,可分析病例299例,其中B-ALL伴重現性細胞遺傳學異常126例,包括B-ALL伴t(9;22)(q34;q11.2)或bcr-abl暘性92例,MLL+ ALL 10例,超二倍體11例,亞二倍體9例,E2A-PBX+ALL 3例,TEL-AML1+ ALL 1例.各組間CD34及髓繫抗原錶達差異有統計學意義.B-ALL非特指型120例.將Ph+ ALL、MLL+ ALL、亞二倍體及E2A-PBX+ ALL患者作為一組,與其他患者相比,前者年齡、WBC、HGB、外週血幼稚細胞比例、乳痠脫氫酶水平均較高.結論 需結閤免疫錶型、細胞遺傳學、分子生物學分析結果以明確ALL診斷.ALL的WHO 2008年分類根據細胞遺傳學對B-ALL作瞭更詳細分類.
목적 연구성인급성림파세포백혈병(ALL)생물학특정,분석WHO 2008년ALL분류적변화.방법 응용류식세포술、R현대상규핵형분석법、RT-PCR기술대초진성인ALL환자진행면역학、세포유전학、분자생물학검측.결과 ①공412례초진성인ALL환자,남성239례,녀성173례.면역표형가분석병례410례,B계357례,T계53례.B계적수계항원표체비례고우T계.수계항원표체여CD34표체상관.②Ph+화(혹)bcr-abl+ALL(간칭Ph+ ALL)환자93례,주요견우CD10+ALL,환자WBC、수계항원표체급CD34양성솔고우Ph- ALL환자.ALL반MLL중배(MLL+)환자12례,주요견우조기전B-ALL.③거WHO 2008년분류표준,가분석병례299례,기중B-ALL반중현성세포유전학이상126례,포괄B-ALL반t(9;22)(q34;q11.2)혹bcr-abl양성92례,MLL+ ALL 10례,초이배체11례,아이배체9례,E2A-PBX+ALL 3례,TEL-AML1+ ALL 1례.각조간CD34급수계항원표체차이유통계학의의.B-ALL비특지형120례.장Ph+ ALL、MLL+ ALL、아이배체급E2A-PBX+ ALL환자작위일조,여기타환자상비,전자년령、WBC、HGB、외주혈유치세포비례、유산탈경매수평균교고.결론 수결합면역표형、세포유전학、분자생물학분석결과이명학ALL진단.ALL적WHO 2008년분류근거세포유전학대B-ALL작료경상세분류.
Objective To investigate the biologic features of adult acute lymphoblastic leukemia (ALL) ,and reclassified our ALL patients according to the 2008 WHO classification. Methods Immunophenotype and cytogenetic/molecular genetic results were obtained by flow cytometry, R-banding and RT-PCR,respectively. Results ① A total of 412 newly diagnosed and previously untreated adult ALL patients, were 239 males and 173 females. Among 410 patients with available immunophenotypic results, 357 were B-ALL and 53 T-ALL Myeloid antigen (MyAg) was higher expression in B-ALL than in T-ALL, and was correlated with the expression of CD34. ②93 Ph+ ALL patients, mainly CD10+ ALL, was associated with high WBC count and MyAg and CD34 expression. MLL rearrangement was found in 12 cases , mainly pro-B ALL .③299 cases could be analysed, according to the 2008 WHO classification of ALL, including 126 B-ALL with recurrent genetic abnormalities, and 120 B-ALL not otherwise specified. Among the 126 B-ALL with recurrent genetic abnormalities, 92 were Ph+ALL, 10 MLL + ALL, 11 hyperdiploid, 9 hypodiploid, 3 E2A-PBX+ , and 1 TEL-AML1 +. Patients with Ph+ , MLL+ , hypodiploid or E2A-PBX+ were associated with older age, higher WBC count, higher HGB, higher peripheral blasts and higher LDH level as compared with other patients.Conclusion Combination of immunophenotype and cytogenetic-molecular profiles can provide a further detailed classification of B-ALL.
