CAJ | 학술논문

本文通过横断面研究,对100例正常人群(一次性)采集外周全血2ml,选用流式细胞技术测定单核细胞群、mCD14平均荧光强度值,行盲法测定.观察正常人群外周血mCD14的表达水平,探讨正常人群mCD14定量研究的方法.结果显示单核细胞与淋巴细胞的比值比单个读取mCD14的平均荧光强度值更具可重复性,集中趋势较好,有利于临床应用和比较研究.此方法可作为流式细胞仪定量检测数据校正的方法参考.
본문통과횡단면연구,대100례정상인군(일차성)채집외주전혈2ml,선용류식세포기술측정단핵세포군、mCD14평균형광강도치,행맹법측정.관찰정상인군외주혈mCD14적표체수평,탐토정상인군mCD14정량연구적방법.결과현시단핵세포여림파세포적비치비단개독취mCD14적평균형광강도치경구가중복성,집중추세교호,유리우림상응용화비교연구.차방법가작위류식세포의정량검측수거교정적방법삼고.
First,peripheral blood (2ml) was acquired all at once by cross--sectional study in 100 normal cases, then the av-erage fluorescence intensity values of monocytes and mCD14 were quantiated by using flow cytometric-immunologic method and blind determination. To observe the expression and to explore the quantitative method of mCD14 in peripheral blood a-mong normal population. The results show that the ratio of mononuclear cells and lymphocytes has better replicability and central tendency than the direct single detection of average fluorescence intensity values of mCD14. The ratio of mononuclear cells and lymphocytes is conducive to clinical applications and comparative study. This method can be used as the reference of quanntative detection of flow eytometry data calibration