CAJ | 학술논문

为建立贵州栽培苦参药材中有效成分苦参碱含星的测定方法,采用HPLC法DiamonsiITM(钻石)C18色谱柱,以乙睛、3%磷酸(70:30)为流动相,流速为1.0 mL/min,柱温25℃,检测波长210 nm.结果显示方法线性关系良好,平均加样回收率98.01%,RSD=1.15%,表明该法简便快速,结果准确,可以作为控制栽培苦参药材质量的方法.
위건립귀주재배고삼약재중유효성분고삼감함성적측정방법,채용HPLC법DiamonsiITM(찬석)C18색보주,이을정、3%린산(70:30)위류동상,류속위1.0 mL/min,주온25℃,검측파장210 nm.결과현시방법선성관계량호,평균가양회수솔98.01%,RSD=1.15%,표명해법간편쾌속,결과준학,가이작위공제재배고삼약재질량적방법.
To establish the determination method of Matrine in Sophora flavescens Ait.DiamonsiITMC18 column was used and Acetonitrile-3%Phosphate acetate(70:30)was used as a mobile phase,velocity-1.0 mL/min;column temperature-25℃,the detective wavelength was 210 nm.This method showed a good linear relationship.The average recoveries was 98.01%,RSD was 1.15%.This method is simple,rapid,accurate,and can be used as the quantitative analysis method for this preparation.