中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2009年
6期
379-382
,共4页
缩宫素%缩宫素受体%前列腺素E2%前列腺素F2alpha
縮宮素%縮宮素受體%前列腺素E2%前列腺素F2alpha
축궁소%축궁소수체%전렬선소E2%전렬선소F2alpha
oxytocin%oxytocin receptor%prostaglandin E2%prostaglandin F2alpha
目的 研究缩宫素(OT)、前列腺素干预对原代培养的子宫平滑肌细胞缩宫素受体(OTR)表达的影响.方法 分别用OT、前列腺素E2(PGE2)、前列腺素F2alpha (PGF2alpha)干预原代人子宫平滑肌细,检测细胞匀浆中的OTR mRNA、蛋白的表达.结果 OT干预前后OTR的表达无显著改变.PGE2、PGF2alpha干预组较未干预组OTR的表达明显增加.PGE2与PGF2alpha共干预组较未干预组及PGE2、PGF2alpha单独干预组OTR的表达明显增加.结论 OT不能增加人子宫平滑肌细胞上OTR的表达,PGE2、PGF2alpha可增加人子宫平滑肌细胞中OTR的表达,且有协同作用.
目的 研究縮宮素(OT)、前列腺素榦預對原代培養的子宮平滑肌細胞縮宮素受體(OTR)錶達的影響.方法 分彆用OT、前列腺素E2(PGE2)、前列腺素F2alpha (PGF2alpha)榦預原代人子宮平滑肌細,檢測細胞勻漿中的OTR mRNA、蛋白的錶達.結果 OT榦預前後OTR的錶達無顯著改變.PGE2、PGF2alpha榦預組較未榦預組OTR的錶達明顯增加.PGE2與PGF2alpha共榦預組較未榦預組及PGE2、PGF2alpha單獨榦預組OTR的錶達明顯增加.結論 OT不能增加人子宮平滑肌細胞上OTR的錶達,PGE2、PGF2alpha可增加人子宮平滑肌細胞中OTR的錶達,且有協同作用.
목적 연구축궁소(OT)、전렬선소간예대원대배양적자궁평활기세포축궁소수체(OTR)표체적영향.방법 분별용OT、전렬선소E2(PGE2)、전렬선소F2alpha (PGF2alpha)간예원대인자궁평활기세,검측세포균장중적OTR mRNA、단백적표체.결과 OT간예전후OTR적표체무현저개변.PGE2、PGF2alpha간예조교미간예조OTR적표체명현증가.PGE2여PGF2alpha공간예조교미간예조급PGE2、PGF2alpha단독간예조OTR적표체명현증가.결론 OT불능증가인자궁평활기세포상OTR적표체,PGE2、PGF2alpha가증가인자궁평활기세포중OTR적표체,차유협동작용.
Purpose To investigate the effects of oxytocin(OT) and prostaglandins intervention on oxytocin receptor(OTR) expression of primary culture of human myometrial smooth muscle cell.Methods Using respectively oxytocin, prostaglandin E2(PGE2),and prostaglandin F2alpha(PGF2alpha) intervened on human myometrial smooth muscle cell.The expression of OTR mRNA and protein of cell hemogenate was examined.Results The expression of OTR between oxytocin intervention group and untreated group was similar.The expression of OTR in cell was significantly higher in the PGE2 or PGF2alpha intervention group than that in the untreated group.The expression of OTR in cell was significantly higher in the PGE2 and PGF2alpha joint intervention group than that in the untreated group and than that in the PGE2 or PGF2alpha individual intervention group.Conclusion Oxytocin didn′t increse the expression of OTR in human myometrial smooth muscle cell.PGE2 and PGF2alpha incresed the expression of oxytocin receptor in human myometrial smooth muscle cell.Furthermore PGE2 and PGF2alpha joint intervention more significantly increased the expression of oxytocin receptor than PGE2 or PGF2alpha individual intervention in human myometrial smooth muscle cell.
