林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2010年
3期
173-177
,共5页
杜娟%南宫自艳%周国娜%高宝嘉
杜娟%南宮自豔%週國娜%高寶嘉
두연%남궁자염%주국나%고보가
美国白蛾%SSR%反应体系
美國白蛾%SSR%反應體繫
미국백아%SSR%반응체계
Hyphantria cunea%SSR%analysis system
An optimization SSR reaction system was established to investigate the genetic diversity of Hyphantria cunea (Drury). A population of Hyphantria cunea in Cangzhou was used to optimize PCR conditions in the SSR system. The results showed that the best condition was 0.40 mmol·L~(-1) dNTP,2.5 mmol·L~(-1) Mg~(2+), 0.32 μmol·L~(-1) primers, 1U Taq polymerase, 30 ～ 60 ng template DNA in 25 μL SSR reaction system and the optimal annealing temperature of primers was 52 ℃. Genetic diversity of five Hyphantria cunea populations was studied with this SSR reaction system, and the result showed the microsatellite primers were high polymorphic and this system was suitable and stable.
