CAJ | 학술논문

背景:目前可供移植的器官日益短缺,但因灌注冷保存所至的原发性移植物无功能仍然有一定的发生率,减少灌注保存所至的器官损伤有较大的临床意义.目的:观察高氧液对大鼠肝移植的保护作用.方法:选用Wistar大鼠40只,按随机数字表法分成2组,即高氧乳酸林格液组(高氧液组)和乳酸林格液(普通灌注液组),每组20只,各组供、受体各半,制备肝、肾、胰器官簇移植模型.高氧液组使用高氧液灌注,普通灌注液组使用普通林格液灌注,比较两组灌注末、肝移植后第1,3天透明质酸质量浓度、谷丙转氨酶活性、CD8+CD28-T细胞含量,以及移植后肝组织病理急性排斥评分.结果与结论:移植前两组透明质酸质量浓度、谷丙转氨酶活性、CD8+CD28-T细胞含量差异无显著性意义(P>0.05).移植后高氧液组透明质酸质量浓度、谷丙转氨酶活性均低于普通灌注液组(P<0.05),CD8+CD28-T细胞含量高于普通灌注液组(P<0.05),高氧液组Banff评分低于普通灌注液组(P<0.05).提示高氧液可减轻缺血再灌注损伤,对大鼠肝移植有一定的保护作用.
배경:목전가공이식적기관일익단결,단인관주랭보존소지적원발성이식물무공능잉연유일정적발생솔,감소관주보존소지적기관손상유교대적림상의의.목적:관찰고양액대대서간이식적보호작용.방법:선용Wistar대서40지,안수궤수자표법분성2조,즉고양유산림격액조(고양액조)화유산림격액(보통관주액조),매조20지,각조공、수체각반,제비간、신、이기관족이식모형.고양액조사용고양액관주,보통관주액조사용보통림격액관주,비교량조관주말、간이식후제1,3천투명질산질량농도、곡병전안매활성、CD8+CD28-T세포함량,이급이식후간조직병리급성배척평분.결과여결론:이식전량조투명질산질량농도、곡병전안매활성、CD8+CD28-T세포함량차이무현저성의의(P>0.05).이식후고양액조투명질산질량농도、곡병전안매활성균저우보통관주액조(P<0.05),CD8+CD28-T세포함량고우보통관주액조(P<0.05),고양액조Banff평분저우보통관주액조(P<0.05).제시고양액가감경결혈재관주손상,대대서간이식유일정적보호작용.
BACKGROUND: Organ for transplantation is insufficient, and primary transplant of nonfunction caused by perfusion cryopreservation occasionally occurs. It is clinically significant to reduce organ damage caused by perfusion preservation. OBJECTIVE: To explore the protective effect of hyperoxic perfusion fluid on liver transplantation in rats. METHODS: A total of 40 Wistar rats were randomly divided two groups (n = 20) and respectively poured with Ringer lactate solution or hyperoxic ringer lactate solution. Each group comprised equal number of donors and recipients to prepare liver, kidney, and pancreas transplantation models. Hyaluronic acid (HA), alanine aminotransferase (ALT) and CD8+CD28- T cells were compared between two groups at the end of perfusion, and 1st and 3rd days after liver transplantation. The acute rejection score of liver tissues were also compared after operation. RESULTS AND CONCLUSION: The HA, ALT and CD8+CD28-T cells were no significantly different between two groups before operation (P> 0.05). The HA and ALT of hyperoxic ringer lactate solution group was significantly Ringer lactate solution group after liver transplant (P < 0.05), but the CD8+CD28-T cells were greater (P < 0.05). The acute rejection scores for liver in hyperoxia liquid group were significantly less than the common liquid group (P< 0.05). Results show that hyperoxic solution can attenuate ischemia/reperfusion injury and protect rats undergoing liver transplantation.