CAJ | 학술논문

目的观察中药复方解毒消癥饮含药血清对SGC-7901胃腺癌细胞凋亡作用的超微结构改变.方法采用解毒消癥饮含药血清,与胃腺癌细胞共培养24 h和72 h,透射电镜观察肿瘤细胞超微结构的变化;以胞质为参照空间,随机摄片并通过SIS软件按体视学原理和方法,测试胃癌细胞中细胞核和线粒体的体积密度(Vv)、形状因子(PE)、平均周长(L)、平均体积(V)及平均表面积(S),比较这些参数在不同组间的差异.结果解毒消癥饮含药血清干预下可见胃癌细胞表面微绒毛减少,细胞体积减小,细胞核异染色质多聚集于核膜下,线粒体体积缩小,嵴减少,电子密度增高;解毒消癥饮含药血清干预24 h的胃癌细胞与对照组比较,细胞核和线粒体Vv、PE、V、S、L有统计学意义(P＜0.05).结论解毒消癥饮含药血清能诱导胃癌细胞凋亡,出现细胞核异染色质边聚、线粒体固缩等凋亡早期的超微结构变化;电镜图像定量分析能客观反映细胞核及线粒体的变化情况.
목적 관찰중약복방해독소징음함약혈청대SGC-7901위선암세포조망작용적초미결구개변.방법 채용해독소징음함약혈청,여위선암세포공배양24 h화72 h,투사전경관찰종류세포초미결구적변화;이포질위삼조공간,수궤섭편병통과SIS연건안체시학원리화방법,측시위암세포중세포핵화선립체적체적밀도(Vv)、형상인자(PE)、평균주장(L)、평균체적(V)급평균표면적(S),비교저사삼수재불동조간적차이.결과 해독소징음함약혈청간예하가견위암세포표면미융모감소,세포체적감소,세포핵이염색질다취집우핵막하,선립체체적축소,척감소,전자밀도증고;해독소징음함약혈청간예24 h적위암세포여대조조비교,세포핵화선립체Vv、PE、V、S、L유통계학의의(P＜0.05).결론 해독소징음함약혈청능유도위암세포조망,출현세포핵이염색질변취、선립체고축등조망조기적초미결구변화;전경도상정량분석능객관반영세포핵급선립체적변화정황.
Objective To study the effect of Jiedu-xiaozheng decoction on theUltrastructure changes and quantitative analysis of SGC-7901 in vitro and its mechanisms. Methods The compound prescription Jiedu-xiaozheng decoction was used to prepare serum containing drug. Serologic pharmacology methods was applied. The decoction was used to co-culture the gastric cells for24 h and 72 h, and the ultrastructure of tumor cells was observed by the transmission electron microscope. Then the parameters of the nucleus and mitochrondria were determined in SGC-7901 cells, including volume density(Vv) ,form factor PE( PE), average volume(V) ,length(L) average surrace area(S),contrasting to cytoplasma. The SIS powervision was applied to analyze their difference. Results Jiedu-xiaozheng decotion can result in reduced microvilli, deflated volume, decreased mitochondrion volume and reduced ridge. The volume density(Vv) ,form factor PE(PE) ,average volume(v) ,length (L) average surrace area(S) reduced in the 24 h group clearly in Jiedu-xiaozheng decoction group compared to control group. Conclusion Jiedu-xiaozheng decoction can significantly induce SGC-7901 cells apoptosis, exhibiting certain early ultrastructure changes such as marginated heterochromatin and mitochondrion pyknosis. The quantity analysis by electronic imaging system may objectively reveal the ultrastructural changes of cell nucleus and mitochondria.