中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2008年
2期
130-133
,共4页
程爱萍%陈绍亮%王洪复%金慰芳%高建军%许长德
程愛萍%陳紹亮%王洪複%金慰芳%高建軍%許長德
정애평%진소량%왕홍복%금위방%고건군%허장덕
成骨细胞,细胞分裂%细胞周期%体外研究%放射疗法%铼%HEDP%大鼠
成骨細胞,細胞分裂%細胞週期%體外研究%放射療法%錸%HEDP%大鼠
성골세포,세포분렬%세포주기%체외연구%방사요법%래%HEDP%대서
Osteoblasts,cell division%Cell cycle%In vitro%Radiotherapy%Rhenium%HEDP%Rats
目的 观察不同剂量188Re-羟基亚乙基二膦酸盐(HEDP)近距离作用对成骨细胞增殖和细胞周期的影响.方法 在体外培养的成骨细胞中加入不同放射性浓度(0,1.85,9.61,48.00,240.50,462.50,832.50和1110.00 kBq/ml)188 Re-HEDP,继续培养24 h后检测细胞的放射性计数,了解成骨细胞摄取188Re-HEDP的能力.用四甲基偶氮唑蓝(MTT)法检测成骨细胞增殖能力.测定细胞碱性磷酸酶(ALP)活性,以明确成骨细胞分化功能.采用流式细胞仪检测细胞周期变化.结果 成骨细胞摄取188Re-HEDP的能力较强,188Re-HEDP≤1110.00 kBq/ml时未观察到结合平台期.受188.Re-HEDP刺激后成骨细胞生长增殖旺盛、分化加强,成骨细胞存活率为(113.67±3.22)%-(122.00±6.58)%,ALP值为(0.42±0.02)～(0.50±0.05)U/L;188Re-HEDP≥33.30 MBq/ml时成骨细胞凋亡率增加[(6.26±0.09)%]并随剂量增大.188Re-HEDP作用后处于合成期的成骨细胞百分率明显增加,为(22.32±2.31)%-(35.58±5.18)%.结论 188Re-HEDP可能刺激成骨细胞增殖和分化,使合成期的细胞百分率增高.188Re-HEDP超过33.30 MBq/ml时引起成骨细胞凋亡,且凋亡率与其放射性浓度呈正相关.
目的 觀察不同劑量188Re-羥基亞乙基二膦痠鹽(HEDP)近距離作用對成骨細胞增殖和細胞週期的影響.方法 在體外培養的成骨細胞中加入不同放射性濃度(0,1.85,9.61,48.00,240.50,462.50,832.50和1110.00 kBq/ml)188 Re-HEDP,繼續培養24 h後檢測細胞的放射性計數,瞭解成骨細胞攝取188Re-HEDP的能力.用四甲基偶氮唑藍(MTT)法檢測成骨細胞增殖能力.測定細胞堿性燐痠酶(ALP)活性,以明確成骨細胞分化功能.採用流式細胞儀檢測細胞週期變化.結果 成骨細胞攝取188Re-HEDP的能力較彊,188Re-HEDP≤1110.00 kBq/ml時未觀察到結閤平檯期.受188.Re-HEDP刺激後成骨細胞生長增殖旺盛、分化加彊,成骨細胞存活率為(113.67±3.22)%-(122.00±6.58)%,ALP值為(0.42±0.02)～(0.50±0.05)U/L;188Re-HEDP≥33.30 MBq/ml時成骨細胞凋亡率增加[(6.26±0.09)%]併隨劑量增大.188Re-HEDP作用後處于閤成期的成骨細胞百分率明顯增加,為(22.32±2.31)%-(35.58±5.18)%.結論 188Re-HEDP可能刺激成骨細胞增殖和分化,使閤成期的細胞百分率增高.188Re-HEDP超過33.30 MBq/ml時引起成骨細胞凋亡,且凋亡率與其放射性濃度呈正相關.
목적 관찰불동제량188Re-간기아을기이련산염(HEDP)근거리작용대성골세포증식화세포주기적영향.방법 재체외배양적성골세포중가입불동방사성농도(0,1.85,9.61,48.00,240.50,462.50,832.50화1110.00 kBq/ml)188 Re-HEDP,계속배양24 h후검측세포적방사성계수,료해성골세포섭취188Re-HEDP적능력.용사갑기우담서람(MTT)법검측성골세포증식능력.측정세포감성린산매(ALP)활성,이명학성골세포분화공능.채용류식세포의검측세포주기변화.결과 성골세포섭취188Re-HEDP적능력교강,188Re-HEDP≤1110.00 kBq/ml시미관찰도결합평태기.수188.Re-HEDP자격후성골세포생장증식왕성、분화가강,성골세포존활솔위(113.67±3.22)%-(122.00±6.58)%,ALP치위(0.42±0.02)～(0.50±0.05)U/L;188Re-HEDP≥33.30 MBq/ml시성골세포조망솔증가[(6.26±0.09)%]병수제량증대.188Re-HEDP작용후처우합성기적성골세포백분솔명현증가,위(22.32±2.31)%-(35.58±5.18)%.결론 188Re-HEDP가능자격성골세포증식화분화,사합성기적세포백분솔증고.188Re-HEDP초과33.30 MBq/ml시인기성골세포조망,차조망솔여기방사성농도정정상관.
objective Radionuclide has been used n the treatment of bone metastasis in the last few decades but its effect-on osteoblasts is unclear.The aim of this work was to investigate the effects of different irradiation doses of 188 Re-hydroxvethylidenediphosphonate(HEDP)on the proliferation,differentiation and apoptosis of osteoblasts.Methods Osteoblasts from SD rats were cultured in vitro.The growing osteoblasts at the fifth generation were irradiated by 188 Re-HEDP at different doses(0,1.85,9.61,48.00, 240.50,462.50.832.50 and 1110.00 kBq/ml).The proliferation of osteoblasts was determined by its functional capability and growth characteristics.which were detected by uptake of 188 Re-HEDP using γ counter and by the method of 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenylte-trazolium bromide(MTT)respectively.The differentiation and apoptosis of osteoblasts were examined by alkaline phosphatase(ALP)and flow cytometry respectively.Results The proliferation and differentiation of osteoblasts were enhanced after188 Re-HEDP irradiation.The S period in cell cycle was augmented.The survival rate of osteoblasts was(113.67±3.22)%～(122.00±6.58)%,the ALP was(0.42±0.02)～(O.50±0.05)U/L.The uptake of188Re-HEDP by osteoblasts did not reach equilibrium when the dose is less than 1110.00 kBq/ml.The apoptosis of osteoblasts occurred [apoptosis rate≥(6.26±0.09)%]when 188 Re-HEDP dose was equal to or greater than 33.30 MBq/ml.The apoptosis rate was positively correlated with the188Re-HEDP activity concentration.Conclusions 188 Re-HEDP played the role in enhancing the proliferation and differentiation of osteoblasts when its irradiation dose was less or equal to 1110.00 kBq/m1.When the 188Re-HEDP dose was equal to or greater than 33.30 MBq/ml.apoptosis of osteoblasts would occur.
