CAJ | 학술논문

目的探讨狗舌草提取物对多发性骨髓瘤U266细胞株的细胞毒作用及其机制.方法 U266细胞株与狗舌草提取物共同培养,用CCK-8试剂检测细胞毒作用,用流式细胞术(FCM)对细胞周期及凋亡进行检测.结果狗舌草提取物对U266细胞有细胞毒作用,半数抑制质量浓度约为3.2μg/ml;狗舌草总提取物影响U266细胞周期,当药物质量浓度为1.25、2.5、5、10 mg/L时G0/G1期细胞减少[(34.12±0.49)%、(38.06±0.63)%、(27.46±0.61)%、(15.91 4-0.32)%],S期[(4.98±0.50)%、(4.01±0.22)%、(4.16±0.15)%、(5.04±0.12)%]、G2/M期[(50.05±1.12)%、(51.27±0.71)%、(51.84±0.73)%、(55.1l±0.25)%]细胞增加;凋亡细胞增加;用Annexin V/PI染色FCM检测,均显示细胞凋亡与狗舌草提取物有剂量依赖关系.结论狗舌草提取物对U266细胞有体外细胞毒作用.其作用机制部分是诱导细胞凋亡.
목적 탐토구설초제취물대다발성골수류U266세포주적세포독작용급기궤제.방법 U266세포주여구설초제취물공동배양,용CCK-8시제검측세포독작용,용류식세포술(FCM)대세포주기급조망진행검측.결과 구설초제취물대U266세포유세포독작용,반수억제질량농도약위3.2μg/ml;구설초총제취물영향U266세포주기,당약물질량농도위1.25、2.5、5、10 mg/L시G0/G1기세포감소[(34.12±0.49)%、(38.06±0.63)%、(27.46±0.61)%、(15.91 4-0.32)%],S기[(4.98±0.50)%、(4.01±0.22)%、(4.16±0.15)%、(5.04±0.12)%]、G2/M기[(50.05±1.12)%、(51.27±0.71)%、(51.84±0.73)%、(55.1l±0.25)%]세포증가;조망세포증가;용Annexin V/PI염색FCM검측,균현시세포조망여구설초제취물유제량의뢰관계.결론 구설초제취물대U266세포유체외세포독작용.기작용궤제부분시유도세포조망.
Objective To study cytotoxic and antineoplastic effect in vitro of tephroseris kirilowii turez, houlub extract on U266 multiple myeloma cell line. Methods U266 cells were cocultured with the tephroseris kirilowii turez, houlub extract. Cytotoxicity assay was used by CCK-8 detection kit. Cell cycle and apoptosis were determined using flow cytometry(FCM) analysis. Results Extract of tephroseris kirilowii turez, houlub showed strong cytotoxicity against U266 cells.The IC50 was about 3.2 mg/L. After exposure of U266 cells to the drug, the distribution of cell cycle was changed compared with that of the controls. When the durg concentration was 1.25, 2.5, 5 and 10 mg/L. respectively, the pencentages of cells in the G0/G1 phase were decreased with (34.12±0.49) %, (38.06 ± 0.63) % , (27.46±0.61) %, (15.91±0.32) %, respectively, while those in the S phase were increased with (4.98± 0.50) %, (4.01±0.22) %, (4.16±0.15) % and (5.04±0.12) % in G2/ M phase were increased with (50.05 ±1.12) %, (51.27±0.71) %, (51.84±0.73) % and (55.11±0.25) %, respectively, and apoptosis cells were increased. Apoptosis of U266 cells inadose-depadeut manner could be deteted with staining of Annexix V FITC/PI testing through FCM. Conclusion Tephroseris kirilowii turez extract showed strong cytotoxic effect on U266 cells. The antineoplsastic mechanism of the drug can be partly due to its induced apoptotic effect.