CAJ | 학술논문

目的探讨抵抗素样分子α(RELMα)在ApoE基因敲除鼠动脉粥样硬化斑块内的表达及其对血管平滑肌细胞(VSMC)增殖、迁移的影响.方法 C57BL/6J ApoE基因敲除鼠及C57BL/6J野生型小鼠各9只,喂食高脂饲料,24周后处死,自主动脉根部至腹主动脉离断血管,石蜡包埋血管后作连续切片,行HE染色及RELMα免疫组化,RT-PCR检测斑块内RELMα mRNA表达,用不同浓度的重组RELMα刺激培养的VSMC,观察VSMC增殖及其迁移情况,采用SPSS 11.0统计软件包,以one-wayANOVA分析各组之间的差异.结果 ApoE基因敲除鼠高脂饲养2,4周后,主动脉根部形成动脉粥样硬化斑块,免疫组化及其RT-PCR可见RELMα蛋白及其mRNA在粥样硬化斑块内明显表达,野生型C57BL/6J鼠血管壁内,未见RELMα讹及其mRNA表达,重组RELMα能刺激体外培养的VSMC增殖(RELMα刺激组cpm分别为[(2811.21±216.89),(4056.87±220.65),(5061.45±335.86),与对照组(1609.58±203.53)比较,P<0.01]重组RELMα也能刺激体外培养的VSMC迁移[RELMα刺激组迁移细胞数分别为(130.54±12.98),(158.39±11.58),(203.50±17.37),与对照组(70.54±11.92)比较,P<0.01].结论 RELMα在动脉粥样硬化斑块内表达,其能促进VSMC增殖及迁移.
목적 탐토저항소양분자α(RELMα)재ApoE기인고제서동맥죽양경화반괴내적표체급기대혈관평활기세포(VSMC)증식、천이적영향.방법 C57BL/6J ApoE기인고제서급C57BL/6J야생형소서각9지,위식고지사료,24주후처사,자주동맥근부지복주동맥리단혈관,석사포매혈관후작련속절편,행HE염색급RELMα면역조화,RT-PCR검측반괴내RELMα mRNA표체,용불동농도적중조RELMα자격배양적VSMC,관찰VSMC증식급기천이정황,채용SPSS 11.0통계연건포,이one-wayANOVA분석각조지간적차이.결과 ApoE기인고제서고지사양2,4주후,주동맥근부형성동맥죽양경화반괴,면역조화급기RT-PCR가견RELMα단백급기mRNA재죽양경화반괴내명현표체,야생형C57BL/6J서혈관벽내,미견RELMα와급기mRNA표체,중조RELMα능자격체외배양적VSMC증식(RELMα자격조cpm분별위[(2811.21±216.89),(4056.87±220.65),(5061.45±335.86),여대조조(1609.58±203.53)비교,P<0.01]중조RELMα야능자격체외배양적VSMC천이[RELMα자격조천이세포수분별위(130.54±12.98),(158.39±11.58),(203.50±17.37),여대조조(70.54±11.92)비교,P<0.01].결론 RELMα재동맥죽양경화반괴내표체,기능촉진VSMC증식급천이.
Objective To explore the expression of resistin-like molecules-a (RELMa) in atherosclerotic plaque of ApoE-/- mouse, and to study the effects of RELMa on the proliferation and migration of vascular smooth musclee cells (VSMCs) . Method Nine ApoE-/- mice and nine C57BL/6J mice were fed with high fat diet. All mice were sacrificed 24 weeks after force feeding. Vessels were dissected from to abdominal aorta. Sections of aortic tissue were stained with HE dyeing and RELMa in aortic tissue was assayed by immunohistochemistry. The expression of RELMa mRNA in vessels was detected by RT-PCR. The effects of different concentration RELMa in different concentrations on the proliferation and migration of VSMCs were detected. Data was expressed as mean ± standard deviation. ANOVA were used for comparison in SPSS 11.0, and changes were considered as statistically significant if P value was less than 0.05. Results Atherosclerosis plaque formed in aortic root of ApoE-/- mice after they were fed with high fat diet for 24 weeks. RELMa protein and RELMa mRNA were found by immunohistochemistry and RT-PCR in atherosclerotic plaque of ApoE-/- mouse. RELMa protein didn't be found in vessels of control mouse. RELMa promoted the proliferation of VSMCs (RELMa groups: 2811. 21 ± 216. 89,4056. 87 ±220.65,5061.45 ± 335.86, vs. control 1609.58 ± 203.53, P < 0.01). RELMa promoted the migration of VSMCs (RELMa groups: 130.54±12.98,158.39±11.58,203.50± 17.37 vs. control:70.54± 11.92, P<0.01).Conclusions RELMa expresses in atherosclerotic plaque of ApoE-/- mouse. RELMa enhances the proliferation and migration of VSMCs of aorta.