中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
10期
865-868
,共4页
姚娟%吴广延%张浪千%李轩%范郑丽%杨艺%隋建峰
姚娟%吳廣延%張浪韆%李軒%範鄭麗%楊藝%隋建峰
요연%오엄연%장랑천%리헌%범정려%양예%수건봉
听觉惊吓反射%内侧前额叶%豚鼠
聽覺驚嚇反射%內側前額葉%豚鼠
은각량하반사%내측전액협%돈서
Acoustic startle reflex%Medial prefrontal cortex%Guinea pig
目的 观察分析内侧前额叶(mPFC)不同亚区在惊吓反射表达中的作用.方法 24只健康雄性英国种白化豚鼠随机分成4组:前扣带回损伤(n=6)及假损伤(n=6)(实验1);缘前皮质损伤/缘前皮质+前扣带回联合损伤(n=6)及假损伤(n=6)(实验2).分别注射利多卡因或生理盐水.采用条件刺激(CS,声音)与非条件刺激(US,气流冲击角膜)配对训练,观察各组听觉惊吓反射(acoustic startle reflex,ASR)变化.结果 实验l表明前扣带回损伤后SR率无显著变化(时间效应F=15.421,P=0.098;组间效应F=14.753,P=0.084).实验2表明单独缘前皮质注射利多卡因后SR率无显著变化(时间效应F=14.975,P=0.178;组间效应F=18.643,P=0.089);但缘前皮质和前扣带回同时注射利多卡因后,SR发生率则有显著下降,并且没有随着训练而恢复(组间效应F=67.743,P=0.009).结论 mPFC中缘前皮质和前扣带回联合损伤导致ASR发生率显著下降,且没有随着训练而恢复,表明mPFC参与了对听觉惊吓反射的调控,但其调控机制有待探讨.
目的 觀察分析內側前額葉(mPFC)不同亞區在驚嚇反射錶達中的作用.方法 24隻健康雄性英國種白化豚鼠隨機分成4組:前釦帶迴損傷(n=6)及假損傷(n=6)(實驗1);緣前皮質損傷/緣前皮質+前釦帶迴聯閤損傷(n=6)及假損傷(n=6)(實驗2).分彆註射利多卡因或生理鹽水.採用條件刺激(CS,聲音)與非條件刺激(US,氣流遲擊角膜)配對訓練,觀察各組聽覺驚嚇反射(acoustic startle reflex,ASR)變化.結果 實驗l錶明前釦帶迴損傷後SR率無顯著變化(時間效應F=15.421,P=0.098;組間效應F=14.753,P=0.084).實驗2錶明單獨緣前皮質註射利多卡因後SR率無顯著變化(時間效應F=14.975,P=0.178;組間效應F=18.643,P=0.089);但緣前皮質和前釦帶迴同時註射利多卡因後,SR髮生率則有顯著下降,併且沒有隨著訓練而恢複(組間效應F=67.743,P=0.009).結論 mPFC中緣前皮質和前釦帶迴聯閤損傷導緻ASR髮生率顯著下降,且沒有隨著訓練而恢複,錶明mPFC參與瞭對聽覺驚嚇反射的調控,但其調控機製有待探討.
목적 관찰분석내측전액협(mPFC)불동아구재량하반사표체중적작용.방법 24지건강웅성영국충백화돈서수궤분성4조:전구대회손상(n=6)급가손상(n=6)(실험1);연전피질손상/연전피질+전구대회연합손상(n=6)급가손상(n=6)(실험2).분별주사리다잡인혹생리염수.채용조건자격(CS,성음)여비조건자격(US,기류충격각막)배대훈련,관찰각조은각량하반사(acoustic startle reflex,ASR)변화.결과 실험l표명전구대회손상후SR솔무현저변화(시간효응F=15.421,P=0.098;조간효응F=14.753,P=0.084).실험2표명단독연전피질주사리다잡인후SR솔무현저변화(시간효응F=14.975,P=0.178;조간효응F=18.643,P=0.089);단연전피질화전구대회동시주사리다잡인후,SR발생솔칙유현저하강,병차몰유수착훈련이회복(조간효응F=67.743,P=0.009).결론 mPFC중연전피질화전구대회연합손상도치ASR발생솔현저하강,차몰유수착훈련이회복,표명mPFC삼여료대은각량하반사적조공,단기조공궤제유대탐토.
Objective To observe and analyze the role of different subfields of medial prefrontal cortex (mPFC) in the expression course of startle reflex.Methods 24 healthy male British kind of albino guinea pigs were randomly divided into 4 groups:anterior cingulated cortex lesion ( n =6) and sham-lesion ( n =6) ( Experiment 1 ) ; prelimbic cortex lesion/joint lesion of prelimbic cortex and anterior cingulated cortex(n=6) and sham-lesion ( n =6) ( Experiment 2 ).The animals were injected lidocaine ( lesion ) or physiological saline ( sham-lesion ).Each group received paired training of conditioned stimulus( CS,a tone) and unconditioned stimulus (US,a air puff),to observe the acoustic startle reflex(ASR) change of these groups.Results As the results of experiment 1 suggested,SR rate did not change significantly after anterior cingulated cortex lesion ( time effect:F =15.421,P =0.098 ; group effect:F =14.753,P =0.084).As the results of experiment 2 suggested,SR rate did not change significantly after prelimbic cortex lesion ( time effect:F =14.975,P =0.178 ; group effect:F =18.643,P =0.089).When prelimbic cortex and anterior cingulated cortex were lesioned at the same time,SR rate declined significantly and didn ' t recover with the following training ( group effect:F =67.743,P =0.009 ).Conclusion Lesions of the prelimbic cortex and anterior cingulated cortex in mPFC cause the significantly decline of acoustic startle reflex( ASR),which don' t recover with the following training.This study indicates that mPFC involves in the regulation of ASR,but the regulation mechanism needs to be discussed.