CAJ | 학술논문

目的了解人类乳头瘤病毒(HPV)感染与交界于食管与胃之间的食管-胃交界处腺癌(AEG)发生的相关性.方法共有106例AEG和60例胃癌患者被纳入本研究,其中包括14例AEGⅡ型和86例AEGⅢ型腺癌.每一位患者均留有可供研究的癌灶和癌旁黏膜组织.应用多聚酶链反应(PCR)检测的引物为高度敏感且识别谱广的SPF1和GP6+.PCR扩增产物经测序确认HPV感染并分型.结果 106例AEG患者和59例胃癌患者的基因组DNA达到检测标准.11例AEG患者和6例胃腺癌患者为HPV阳性,阳性率分别为10.4%和10.2%(P=0.966).AEG和胃癌患者在HPV总感染率方面差异无统计学意义.结论 HPV在AEGⅡ型、Ⅲ型以及胃腺癌的形成中并不发挥致病性作用.
목적 료해인류유두류병독(HPV)감염여교계우식관여위지간적식관-위교계처선암(AEG)발생적상관성.방법 공유106례AEG화60례위암환자피납입본연구,기중포괄14례AEGⅡ형화86례AEGⅢ형선암.매일위환자균류유가공연구적암조화암방점막조직.응용다취매련반응(PCR)검측적인물위고도민감차식별보엄적SPF1화GP6+.PCR확증산물경측서학인HPV감염병분형.결과 106례AEG환자화59례위암환자적기인조DNA체도검측표준.11례AEG환자화6례위선암환자위HPV양성,양성솔분별위10.4%화10.2%(P=0.966).AEG화위암환자재HPV총감염솔방면차이무통계학의의.결론 HPV재AEGⅡ형、Ⅲ형이급위선암적형성중병불발휘치병성작용.
Objective To determine if human paillomavirus (HPV) infection could be associated with the development of adenocarcinoma of esophagogastric junction (AEG). Methods A total of 106 consecutive AEG patients and 59 consecutive gastric carcinoma patients who accepted surgery at our department between January 2004 and December 2006 were selected. Specimens from tumors and uninvolved mucosa were obtained intra-operatively. Genomic DNA was extracted by the phenol-chloroform-isoamyl alcohol extraction protocol. The HPV infection was determined by PCR using primer set SPF1 and GP6 +.Specificity of the amplified products was confirmed by sequencing. Results The HPV infection rates in AEG and gastric cancer were 10.4% (11/106) and 10.2% (6/59) separately (P=0.966). Conclusion HPV is not a major factor in the carcinogenesis of AEG type Ⅱ , AEG type Ⅲ or stomach.