中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2011年
3期
210-216
,共7页
张辰星%阴正勤%翁传煌%曾玉晓
張辰星%陰正勤%翁傳煌%曾玉曉
장신성%음정근%옹전황%증옥효
视网膜双极细胞%视网膜炎,色素性%大鼠%电生理学
視網膜雙極細胞%視網膜炎,色素性%大鼠%電生理學
시망막쌍겁세포%시망막염,색소성%대서%전생이학
Retinal bipolar cells%Retinitis pigmentosa%Rats%Electrophysiology
目的 探讨在以视杆细胞变性为起始的视网膜色素变性疾病中,异常突起对视网膜ON型双极细胞(ON-RBCs)电生理特性改变的影响.方法 实验研究.对生后60 d,生后90 d的皇家外科学院(RCS)大鼠(RCS)和对照组大鼠(CTR)视网膜冰冻切片,行免疫双标荧光染色.采用Lucifer Yellow对上述天龄段的ON-RBCs进行单细胞内染色.对生后60 d,生后90 d不同发育阶段的RCS大鼠ON-RBCs(RCS-ON-RBCs)和对照组大鼠的ON-RBCs(CTR-ON-RBCs)以及对照组大鼠OFF型双极细胞(CTR-OFF-RBCs)进行全细胞膜片钳记录,研究其被动电生理学特性、外向型离子电流的性质和变化.用t检验对数据进行统计学分析.结果 RCS大鼠视网膜色素变性过程中,RCS-ON-RBCs向不同方向伸出mGluR6表达阳性的异常突起,其周围存在突触活动迹象.视网膜变性过程中RCS-ON-RBCs细胞的被动电生理学特性呈现出幼稚化的表现,生后60 d,RCS-ON-RBCs、CTR-ON-RBCs大鼠的静息膜电位分别为(-61.8±3.07)、(-50.44±1.36)mV,生后90 d分别为(-63.1±2.59)、(-48.37±3.69)mV,与对照组相比差异均有统计学意义(t=2.191,2.435,5.817,6.912;P＜0.05).生后60 d,RCS-ON-RBCs、CTR-ON-RBCs和对CTR-OFF-RBCs组大鼠的输入阻抗分别为(323.3±42.6)、(337.6±71.3)MΩ,生后90 d分别为(321.2±58.6)、(340.3±62.8)MΩ,与对照组相比差异均有统计学意义(t=3.561,1.987,5.211,4.034;P＜O.05).在给予去极化脉冲的阶跃刺激,在视网膜变性的中晚期RCS-ON-RBCs外向型电流幅值不同于CTR-ON-RBCs(t=5.561,6.341;P＜0.05)及CTR-OFF-RBCs(t=5.357,6.997;P＜0.05).结论 来源于RCS-ON-RBCs的异常突起具有一定的信号传递功能.
目的 探討在以視桿細胞變性為起始的視網膜色素變性疾病中,異常突起對視網膜ON型雙極細胞(ON-RBCs)電生理特性改變的影響.方法 實驗研究.對生後60 d,生後90 d的皇傢外科學院(RCS)大鼠(RCS)和對照組大鼠(CTR)視網膜冰凍切片,行免疫雙標熒光染色.採用Lucifer Yellow對上述天齡段的ON-RBCs進行單細胞內染色.對生後60 d,生後90 d不同髮育階段的RCS大鼠ON-RBCs(RCS-ON-RBCs)和對照組大鼠的ON-RBCs(CTR-ON-RBCs)以及對照組大鼠OFF型雙極細胞(CTR-OFF-RBCs)進行全細胞膜片鉗記錄,研究其被動電生理學特性、外嚮型離子電流的性質和變化.用t檢驗對數據進行統計學分析.結果 RCS大鼠視網膜色素變性過程中,RCS-ON-RBCs嚮不同方嚮伸齣mGluR6錶達暘性的異常突起,其週圍存在突觸活動跡象.視網膜變性過程中RCS-ON-RBCs細胞的被動電生理學特性呈現齣幼稚化的錶現,生後60 d,RCS-ON-RBCs、CTR-ON-RBCs大鼠的靜息膜電位分彆為(-61.8±3.07)、(-50.44±1.36)mV,生後90 d分彆為(-63.1±2.59)、(-48.37±3.69)mV,與對照組相比差異均有統計學意義(t=2.191,2.435,5.817,6.912;P＜0.05).生後60 d,RCS-ON-RBCs、CTR-ON-RBCs和對CTR-OFF-RBCs組大鼠的輸入阻抗分彆為(323.3±42.6)、(337.6±71.3)MΩ,生後90 d分彆為(321.2±58.6)、(340.3±62.8)MΩ,與對照組相比差異均有統計學意義(t=3.561,1.987,5.211,4.034;P＜O.05).在給予去極化脈遲的階躍刺激,在視網膜變性的中晚期RCS-ON-RBCs外嚮型電流幅值不同于CTR-ON-RBCs(t=5.561,6.341;P＜0.05)及CTR-OFF-RBCs(t=5.357,6.997;P＜0.05).結論 來源于RCS-ON-RBCs的異常突起具有一定的信號傳遞功能.
목적 탐토재이시간세포변성위기시적시망막색소변성질병중,이상돌기대시망막ON형쌍겁세포(ON-RBCs)전생리특성개변적영향.방법 실험연구.대생후60 d,생후90 d적황가외과학원(RCS)대서(RCS)화대조조대서(CTR)시망막빙동절편,행면역쌍표형광염색.채용Lucifer Yellow대상술천령단적ON-RBCs진행단세포내염색.대생후60 d,생후90 d불동발육계단적RCS대서ON-RBCs(RCS-ON-RBCs)화대조조대서적ON-RBCs(CTR-ON-RBCs)이급대조조대서OFF형쌍겁세포(CTR-OFF-RBCs)진행전세포막편겸기록,연구기피동전생이학특성、외향형리자전류적성질화변화.용t검험대수거진행통계학분석.결과 RCS대서시망막색소변성과정중,RCS-ON-RBCs향불동방향신출mGluR6표체양성적이상돌기,기주위존재돌촉활동적상.시망막변성과정중RCS-ON-RBCs세포적피동전생이학특성정현출유치화적표현,생후60 d,RCS-ON-RBCs、CTR-ON-RBCs대서적정식막전위분별위(-61.8±3.07)、(-50.44±1.36)mV,생후90 d분별위(-63.1±2.59)、(-48.37±3.69)mV,여대조조상비차이균유통계학의의(t=2.191,2.435,5.817,6.912;P＜0.05).생후60 d,RCS-ON-RBCs、CTR-ON-RBCs화대CTR-OFF-RBCs조대서적수입조항분별위(323.3±42.6)、(337.6±71.3)MΩ,생후90 d분별위(321.2±58.6)、(340.3±62.8)MΩ,여대조조상비차이균유통계학의의(t=3.561,1.987,5.211,4.034;P＜O.05).재급여거겁화맥충적계약자격,재시망막변성적중만기RCS-ON-RBCs외향형전류폭치불동우CTR-ON-RBCs(t=5.561,6.341;P＜0.05)급CTR-OFF-RBCs(t=5.357,6.997;P＜0.05).결론 래원우RCS-ON-RBCs적이상돌기구유일정적신호전체공능.
Objective To study the influence of the ectopic synapse for electrophysiological characteristics modification in ON retinal bipolar cells (ON-RBCs) of RCS rat. Methods Immunofluorescence of the retinal frozen section was taken in P60 d, P90 d of RCS rat(RCS) and control rat (CTR) with the anti-mGluR6 and anti-Synaptophysin, Lucifer Yellow staining solo ON-RBCs was taken in all the group. The whole cell recording was performed in the retinal slice of P60 d, P90 d in RCS and CTR. The modification of the passive membrane properties and the outward currents properties in RCS-ON-RBCs, CTRON-RBCs and CTR-OFF-RBCs were observed. Results RCS-ON-RBCs stretched out the ectopic neurite in different direction and the activity of synapse could be detected around the ectopic neurite. From Pn6Od,passive membrane properties of RCS-ON-RBCs kept immature,The RMP in RCS-ON-RBCs and CTR-ON-RBCs were (-61.8±3.07) 、(-50.44±1.36)mV and (-63.1±2.59)、(-48.37±3.69) maV when P60 d and P90 d, there ware significantly higher than CTR group(t=2. 191,2.435,5. 817,6. 912;P＜0. 05). The IR in RCS-ON-RBCs and CTR-ON-RBCs were (323.3±42.6)、 (337.6±71.3) MΩ and (321.2±-58.6)、 (340.3±62.8) MΩ when P60 d and P90 d, there ware significantly higher than CTR group(t=3.561,1.987,5.211,4.034;P＜0.05). Outward currents were recorded when giving hyper-and depolarized voltage steps. In retinal degeneration, the amplitude of outward currents in RCS-ON-RBCs is significantly different with CTR-ON-RBCs (t=5.561,6.341; P＜0. 05) or CTR-OFF-RBCs (t=5.357,6.997; P＜0. 05). Conclusion The ectopic neurite from RCS-ON-RBCs has the possibility for translating the signal. In retinitis pigmentosa, the modification of electrophysiology characteristics in RCS-ON-RBCs was significantly different with CTR-ON-RBCs and CTR-OFF-RBCs. Influence with the ectopic neurite is the possible cause.
