CAJ | 학술논문

以雷公藤绿色嫩叶诱导产生的愈伤组织为外植体,通过正交试验设计,研究了不同培养基对雷公藤优良无性系不定芽诱导、不定芽增殖和生根的影响.结果表明:不同浓度的激素组合对雷公藤愈伤组织不定芽诱导培养的影响显著,最优诱导培养基为MS+IAA 0.2 mg·L~(-1)+KT 0.5 mg·L~(-1)+6-BA 1.5 mg·L~(-1);不同浓度的激素组合对雷公藤不定芽增殖培养的影响极显著,最佳增殖培养基为MS+NAA 0.1 mg·L~(-1)+KT 0.1 mg·L~(-1)+6-BA 1.0 mg·L~(-1);不同培养基对雷公藤生根培养的影响不显著,生根培养适合的培养基为1/2MS+NAA 2.0 mg·L~(-1)+KT 0.1 mg·L~(-1).
이뢰공등록색눈협유도산생적유상조직위외식체,통과정교시험설계,연구료불동배양기대뢰공등우량무성계불정아유도、불정아증식화생근적영향.결과표명:불동농도적격소조합대뢰공등유상조직불정아유도배양적영향현저,최우유도배양기위MS+IAA 0.2 mg·L~(-1)+KT 0.5 mg·L~(-1)+6-BA 1.5 mg·L~(-1);불동농도적격소조합대뢰공등불정아증식배양적영향겁현저,최가증식배양기위MS+NAA 0.1 mg·L~(-1)+KT 0.1 mg·L~(-1)+6-BA 1.0 mg·L~(-1);불동배양기대뢰공등생근배양적영향불현저,생근배양괄합적배양기위1/2MS+NAA 2.0 mg·L~(-1)+KT 0.1 mg·L~(-1).
By orthogonal experiment the tender green leaves were used as explants to study the effect of different medium on adventitious buds induction, adventitious buds proliferation and rooting experiment of Tripterygium wilfordii superior clone. The results showed that there was a significant impact to T.wilfordii adventitious buds induction cultivated on different concentrations of hormone combinations. The optimal induction medium was MS+IAA 0.2 mg·L~(-1)+KT 0.5 mg·L~(-1)+6-BA 1.5 mg·L~(-1). There was a very significant impact to T.wilfordii adventitious buds proliferation cultivated on different concentrations of hormone combinations. The optimal proliferation medium was MS+NAA 0.1 mg·L~(-1)+KT 0.1 mg·L~(-1)+6-BA 1.0 mg·L~(-1). There was not significant impact to T.wilfordii rooting cultivated in different treatments, the adaptive rooting medium was 1/2MS+NAA 2.0 mg·L~(-1)+KT 0.1 mg·L~(-1).