CAJ | 학술논문

目的:利用小鼠感染模型筛选与鉴定幽门螺杆菌SS1株的外膜蛋白抗原.方法:提取SS1株的外膜蛋白进行双向电泳,用幽门螺杆菌感染的小鼠血清作免疫印迹实验,将阳性反应蛋白点进行质谱鉴定分析,将肽质量指纹谱数据输入互联网上的蛋白质数据库进行检索.结果:获得32种抗原相关蛋白.通过与已有报道的幽门螺杆菌感染人抗原比较分析,发现大部分典型的保护性抗原在本实验中都可以检测到.结论:幽门螺杆菌感染的小鼠模型适用于人用保护性幽门螺杆菌抗原的筛选;而且此研究中得到的相关抗原蛋白对于寻找与鉴定幽门螺杆菌未知保护性抗原也有参考价值.
목적:이용소서감염모형사선여감정유문라간균SS1주적외막단백항원.방법:제취SS1주적외막단백진행쌍향전영,용유문라간균감염적소서혈청작면역인적실험,장양성반응단백점진행질보감정분석,장태질량지문보수거수입호련망상적단백질수거고진행검색.결과:획득32충항원상관단백.통과여이유보도적유문라간균감염인항원비교분석,발현대부분전형적보호성항원재본실험중도가이검측도.결론:유문라간균감염적소서모형괄용우인용보호성유문라간균항원적사선;이차차연구중득도적상관항원단백대우심조여감정유문라간균미지보호성항원야유삼고개치.
Objective:To screen antigen of Helicobacter pylori outer membrane proteins by murine infection model.Methods:Parallel two-dimensional gel electrophoresis (2D) of outer membrane proteins extracted from Helicobacter pylori strain SS1 was performed.Western blot of a duplicate 2D gel hybridized with serum from H.pylori-infected murine was employed.Immunogenic H.pylori proteins identified in this way were digested in gel by trypsin and the mass of generated peptides were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).The data obtained from peptide mass finger-printing (PMF) were searched using the internet available database.Results:32 proteins were identified and they are in good agreement with typical protective antigens which reacted with serum from H.pylori-infected patients.Conclusion:The results suggest that murine model of H.pylori may be valid to screen antigens for human vaccination and the proteins identified in this paper are valuable for the selection of H.pylori protective antigens as well.