中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2010年
4期
277-285
,共9页
苗族%HLA-A%-B%-DRB1%等位基因%单倍型%基因多态性
苗族%HLA-A%-B%-DRB1%等位基因%單倍型%基因多態性
묘족%HLA-A%-B%-DRB1%등위기인%단배형%기인다태성
Miao ethnic group%HLA-A,-B,and -DRB1%allele%haplotype%gene polymorphism
目的:探讨湖南苗族人群HLA-A, -B和-DRB1位点等位基因及单倍型遗传多态性. 方法:应用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSO) 反向流式芯片方法对154例湖南苗族健康个体进行HLA-A, -B和-DRB1位点中低分辨基因分型,计算等位基因频率和单倍型频率,并与其他人群进行比较. 结果:154例湖南苗族个体的HLA-A,B,DRB1基因座分别检出等位基因10,25,13种,频率较高的等位基因有A*11(38.96%),A*02(27.27%),A*24(18.83%),B*40(60)(17.53%),B*46(13.96%),B*15(75)(11.69%),DRB1*09(15.26%),DRB1*12(15.26%),DRB1*15(15.26%)及DRB1*04(12.66%)等;高频的单倍型有A11-B60(9.60%),A2-B46(9.27%),A11-B75(9.22%),B75-DR12(6.31%),A11-DR12(9.62%),A11-DR4(9.07%),A11-DR15(6.69%),A11-B75-DR12(5.43%),A11-B60-DR4(4.24%),A2-B46-DR9(3.71%)等.与国内其他人群比较显示,湖南苗族与南方人群HLA多态性较为相近. 结论:湖南苗族人群HLA等位基因及单倍型具有较高的遗传多态性,其分布具有中国南方人群的特点.
目的:探討湖南苗族人群HLA-A, -B和-DRB1位點等位基因及單倍型遺傳多態性. 方法:應用聚閤酶鏈反應-序列特異性寡覈苷痠探針(PCR-SSO) 反嚮流式芯片方法對154例湖南苗族健康箇體進行HLA-A, -B和-DRB1位點中低分辨基因分型,計算等位基因頻率和單倍型頻率,併與其他人群進行比較. 結果:154例湖南苗族箇體的HLA-A,B,DRB1基因座分彆檢齣等位基因10,25,13種,頻率較高的等位基因有A*11(38.96%),A*02(27.27%),A*24(18.83%),B*40(60)(17.53%),B*46(13.96%),B*15(75)(11.69%),DRB1*09(15.26%),DRB1*12(15.26%),DRB1*15(15.26%)及DRB1*04(12.66%)等;高頻的單倍型有A11-B60(9.60%),A2-B46(9.27%),A11-B75(9.22%),B75-DR12(6.31%),A11-DR12(9.62%),A11-DR4(9.07%),A11-DR15(6.69%),A11-B75-DR12(5.43%),A11-B60-DR4(4.24%),A2-B46-DR9(3.71%)等.與國內其他人群比較顯示,湖南苗族與南方人群HLA多態性較為相近. 結論:湖南苗族人群HLA等位基因及單倍型具有較高的遺傳多態性,其分佈具有中國南方人群的特點.
목적:탐토호남묘족인군HLA-A, -B화-DRB1위점등위기인급단배형유전다태성. 방법:응용취합매련반응-서렬특이성과핵감산탐침(PCR-SSO) 반향류식심편방법대154례호남묘족건강개체진행HLA-A, -B화-DRB1위점중저분변기인분형,계산등위기인빈솔화단배형빈솔,병여기타인군진행비교. 결과:154례호남묘족개체적HLA-A,B,DRB1기인좌분별검출등위기인10,25,13충,빈솔교고적등위기인유A*11(38.96%),A*02(27.27%),A*24(18.83%),B*40(60)(17.53%),B*46(13.96%),B*15(75)(11.69%),DRB1*09(15.26%),DRB1*12(15.26%),DRB1*15(15.26%)급DRB1*04(12.66%)등;고빈적단배형유A11-B60(9.60%),A2-B46(9.27%),A11-B75(9.22%),B75-DR12(6.31%),A11-DR12(9.62%),A11-DR4(9.07%),A11-DR15(6.69%),A11-B75-DR12(5.43%),A11-B60-DR4(4.24%),A2-B46-DR9(3.71%)등.여국내기타인군비교현시,호남묘족여남방인군HLA다태성교위상근. 결론:호남묘족인군HLA등위기인급단배형구유교고적유전다태성,기분포구유중국남방인군적특점.
Objective To investigate HLA-A, -B, and -DRB1 gene polymorphism in the Miao ethnic group in Hunan, China. Methods PCR-sequence specific oligonucleotide probes (SSO) reverse flow chip method was used to type HLA-A, -B, and -DRB1 genes of 154 unrelated healthy Miao ethnic individuals in Hunan. The allele and haplotype frequencies were calculated and compared with other populations in China. Results A total of 10 HLA-A, 25 HLA-B, and 13 HLA-DRB1 alleles were observed in the population. The higher frequency alleles included A*11(38.96%), A*02(27.27%), A*24(18.83%), B*40(60)(17.53%), B*46(13.96%), B*15(75)(11.69%), DRB1*09(15.26%), DRB1*12(15.26%), DRB1*15(15.26%), and DRB1*04(12.66%). The most frequent haplotypes were A11-B60(9.60%), A2-B46(9.27%), A11-B75(9.22%), B75-DR12(6.31%), A11-DR12(9.62%), A11-DR4(9.07%), A11-DR15(6.69%), A11-B75-DR12(5.43%), A11-B60-DR4(4.24%), and A2-B46-DR9(3.71%). Compared with other populations in China, HLA gene polymorphism of Hunan Miao population was close to that of southern population. Conclusion HLA loci are highly polymorphic in the Miao population of Hunan, and their distribution has the character of South China population.
