CAJ | 학술논문

背景:关于雌激素对骨髓基质干细胞作用的报道尚不多.目的:观察乙烯雌酚对兔骨髓基质干细胞成骨分化的影响.方法:体外培养骨髓基质干细胞,用0,10-7,10-6,10-5 mol/L浓度乙烯雌酚干预,并设地塞米松10-8 mol/L、β-甘油磷酸钠10 mmol/L、维生素C 50 mg/L为阳性对照.结果与结论:乙烯雌酚干预培养24,48,72 h,10-6 mol/L组显著促进了骨髓基质干细胞增殖(P < 0.01).干预48 h 10-5 mol/L组显著抑制细胞增殖,干预72 h 10-7 mol/L组显著抑制细胞增殖(P < 0.01).10-7 mol/L组乙烯雌酚干预25 d后开始出现钙化结节;10-7,10-6 mol/L组干预14,21 d碱性磷酸酶活性显著增加.证实乙烯雌酚能促进兔骨髓基质干细胞的成骨分化.
배경:관우자격소대골수기질간세포작용적보도상불다.목적:관찰을희자분대토골수기질간세포성골분화적영향.방법:체외배양골수기질간세포,용0,10-7,10-6,10-5 mol/L농도을희자분간예,병설지새미송10-8 mol/L、β-감유린산납10 mmol/L、유생소C 50 mg/L위양성대조.결과여결론:을희자분간예배양24,48,72 h,10-6 mol/L조현저촉진료골수기질간세포증식(P < 0.01).간예48 h 10-5 mol/L조현저억제세포증식,간예72 h 10-7 mol/L조현저억제세포증식(P < 0.01).10-7 mol/L조을희자분간예25 d후개시출현개화결절;10-7,10-6 mol/L조간예14,21 d감성린산매활성현저증가.증실을희자분능촉진토골수기질간세포적성골분화.
BACKGROUND: There are fewer reports about estrogen effects on bone marrow stromal cells (BMSCs). OBJECTIVE: To study the effect of diethylstilbestrol on the osteogenic differentiation of rabbit BMSCs. METHODS: Rabbit BMSCs cultured in vitro were intervened with 0, 10-7, 10-6, 10-5 mol/L diethylstilbestrol, and BMSCs cultured with dexamethasone 10-8 mol/L, β-sodium glycerophosphate 10 mmol/L, and vitamin C 50 mg/L were used as positive controls. RESULTS AND CONCLUSION: 10-6 mol/L diethylstilbestrol significantly improved the proliferative ability of BMSCs at 24, 48, and 72 hours after intervention (P < 0.01). 10-5 mol/L diethylstilbestrol significantly inhibited the proliferation of BMSCs at 48 hours after intervention as well as 10-7 mol/L diethylstilbestrol at 72 hours (P < 0.01). Mineralized nodular structures formed at 25 days after intervention with 10-7 mol/L diethylstilbestrol. Alkaline phosphatase activities were remarkably increased at 14 and 21 days after intervention with 10-7, 10-6 mol/L diethylstilbestrol. It has been proved that diethylstilbestrol has an enhancing effect on the osteogenic differentiation of rabbit BMSCs.