中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
2期
127-130
,共4页
谭伟坚%林仲秋%张晓薇%陈国勤
譚偉堅%林仲鞦%張曉薇%陳國勤
담위견%림중추%장효미%진국근
细胞周期素D1%Ki-67抗原%宫颈上皮内瘤样病变%宫颈鳞癌%人乳头瘤病毒
細胞週期素D1%Ki-67抗原%宮頸上皮內瘤樣病變%宮頸鱗癌%人乳頭瘤病毒
세포주기소D1%Ki-67항원%궁경상피내류양병변%궁경린암%인유두류병독
Cell cycle protein D1%Ki-67 antigen%Cervical intraepithelial neoplasia%Cervical squamous cell carcinoma%Human papilloma virus
目的 研究细胞周期素D1(cyclin D1)、抗原Ki-67在宫颈上皮内瘤样病变(CIN)和宫颈鳞癌发生发展中作用及其与人乳头瘤病毒(HPV)感染转归的关系.方法 2002年1月至2006年12月广州医学院第一附属医院HPV阳性患者104例,分2组:(1)研究组:82例,即病理确诊CIN Ⅰ组17例、CINⅡ组19例、CINⅢ组23例、宫颈鳞癌组23例.(2)对照组:柱状上皮异位22例.应用EnVision法检测宫颈病变组织中cyclin D1、Ki-67蛋白的表达,杂交捕获试验检测宫颈分泌物或阴道残端中HPV感染情况,随访各组患者术后1年内的HPV变化.结果 (1)cyclin D1在各组宫颈组织细胞核内均有表达.其阳性率CINⅢ组[82.61%(19/23)]、宫颈鳞癌组[86.96%(20/23)]与对照组[27.27%(6/22)]、CINⅠ组[58.82%(10/17)]比较,差异有统计学意义(P<0.05),在宫颈鳞癌组与CINⅡ组[68.42%(13/19)]阳性率比较差异有统计学意义(P<0.05).(2)Ki-67在各组宫颈组织细胞核内均有表达,其对照组阳性率[31.82%(7/22)]与CINⅢ组[86.96%(20/23)]、宫颈鳞癌组[91.30%(21/23)]比较差异有统计学意义(P<0.05),而在宫颈鳞癌组与CINⅠ组[58.82%(10/17)]、CIN Ⅱ组[63.16%(12/19)]比较差异有统计学意义(P<0.05).(3)术后1年内各组HPV的转阴率分别与cyclin D1、Ki-67的表达强度呈负相关(rs=-0.299,rs=-0.367,P<0.05).结论 cyclinD1和Ki-67在CIN和宫颈鳞癌的细胞增殖活动中起作用,且两者可能与HPV感染转阴率有关.
目的 研究細胞週期素D1(cyclin D1)、抗原Ki-67在宮頸上皮內瘤樣病變(CIN)和宮頸鱗癌髮生髮展中作用及其與人乳頭瘤病毒(HPV)感染轉歸的關繫.方法 2002年1月至2006年12月廣州醫學院第一附屬醫院HPV暘性患者104例,分2組:(1)研究組:82例,即病理確診CIN Ⅰ組17例、CINⅡ組19例、CINⅢ組23例、宮頸鱗癌組23例.(2)對照組:柱狀上皮異位22例.應用EnVision法檢測宮頸病變組織中cyclin D1、Ki-67蛋白的錶達,雜交捕穫試驗檢測宮頸分泌物或陰道殘耑中HPV感染情況,隨訪各組患者術後1年內的HPV變化.結果 (1)cyclin D1在各組宮頸組織細胞覈內均有錶達.其暘性率CINⅢ組[82.61%(19/23)]、宮頸鱗癌組[86.96%(20/23)]與對照組[27.27%(6/22)]、CINⅠ組[58.82%(10/17)]比較,差異有統計學意義(P<0.05),在宮頸鱗癌組與CINⅡ組[68.42%(13/19)]暘性率比較差異有統計學意義(P<0.05).(2)Ki-67在各組宮頸組織細胞覈內均有錶達,其對照組暘性率[31.82%(7/22)]與CINⅢ組[86.96%(20/23)]、宮頸鱗癌組[91.30%(21/23)]比較差異有統計學意義(P<0.05),而在宮頸鱗癌組與CINⅠ組[58.82%(10/17)]、CIN Ⅱ組[63.16%(12/19)]比較差異有統計學意義(P<0.05).(3)術後1年內各組HPV的轉陰率分彆與cyclin D1、Ki-67的錶達彊度呈負相關(rs=-0.299,rs=-0.367,P<0.05).結論 cyclinD1和Ki-67在CIN和宮頸鱗癌的細胞增殖活動中起作用,且兩者可能與HPV感染轉陰率有關.
목적 연구세포주기소D1(cyclin D1)、항원Ki-67재궁경상피내류양병변(CIN)화궁경린암발생발전중작용급기여인유두류병독(HPV)감염전귀적관계.방법 2002년1월지2006년12월엄주의학원제일부속의원HPV양성환자104례,분2조:(1)연구조:82례,즉병리학진CIN Ⅰ조17례、CINⅡ조19례、CINⅢ조23례、궁경린암조23례.(2)대조조:주상상피이위22례.응용EnVision법검측궁경병변조직중cyclin D1、Ki-67단백적표체,잡교포획시험검측궁경분비물혹음도잔단중HPV감염정황,수방각조환자술후1년내적HPV변화.결과 (1)cyclin D1재각조궁경조직세포핵내균유표체.기양성솔CINⅢ조[82.61%(19/23)]、궁경린암조[86.96%(20/23)]여대조조[27.27%(6/22)]、CINⅠ조[58.82%(10/17)]비교,차이유통계학의의(P<0.05),재궁경린암조여CINⅡ조[68.42%(13/19)]양성솔비교차이유통계학의의(P<0.05).(2)Ki-67재각조궁경조직세포핵내균유표체,기대조조양성솔[31.82%(7/22)]여CINⅢ조[86.96%(20/23)]、궁경린암조[91.30%(21/23)]비교차이유통계학의의(P<0.05),이재궁경린암조여CINⅠ조[58.82%(10/17)]、CIN Ⅱ조[63.16%(12/19)]비교차이유통계학의의(P<0.05).(3)술후1년내각조HPV적전음솔분별여cyclin D1、Ki-67적표체강도정부상관(rs=-0.299,rs=-0.367,P<0.05).결론 cyclinD1화Ki-67재CIN화궁경린암적세포증식활동중기작용,차량자가능여HPV감염전음솔유관.
Objective To investigate the expressions of cell cycle protein D1 (cyclin D1) and proliferation cell nuclear antigen (Ki-67) in cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of cervix as related to prognosis of HPV infection. Methods Between January 2002 and December 2006,104 patients with positive human pappilloma virus (HPV) in The First Affiliated Hospital of Guangzhou Medical College were divided into two groups:the study group (n=82) comprising 17 cases of CIN Ⅰ,19 of CIN Ⅱ,23 of CIN Ⅲ and 23 of SCC (all confirmed by pathology),and the control group comprising 22 cases of cylindrical epithelium dystopia. Immnunohistochemical En Vision assay was used to detect the expressions of cyclin D1 and Ki-67,and hybird capture Ⅱ was used to detect HPV,in cervical discharge or virginal stump. All the patients were followed up for status of HPV within one year after operation. Results Expression of cyclin D1 was found in nuclei of cervical tissue cells of all the groups,including 82.61% (19/23) of CIN Ⅲ lesions and 86.96% (20/23) of SCC,compared significantly with 27.27%(6/22) of cylindrical epithelium dystopia and 58.82% (10/17) of CIN Ⅰ (P<0.05). More cyclin D1was expressed in SCC than that in CIN Ⅱ (P<0.05). Expression of Ki-67 was found in nuclei of cervical tissue cells of all the groups,including 86.96%(20/23) of CIN Ⅲ and 91.30% (21/23) of SCC compared significantly with 31.82% (7/22) of cylindrical epithelium dystopia (P<0.05). The rate of Ki-67 expression in SCC also compared significantly with that in CIN Ⅰ[58.82%(10/17)]and in CIN Ⅱ[63.16% (12/19)](P<0.05). Within one year after operation,the conversion of HPV was negatively correlated with level of cyclin D1 or Ki-67 expression (rs=-0.299,rs=-0.367,P<0.05). Conclusion cyclin D1 and Ki-67 may have a role in the proliferation of CIN and SCC cells,and in HPV conversion.
