中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2008年
5期
469-474
,共6页
熊艳%廖晓星%罗景云%陈国伟%何小洪%谢强%方典秋%马虹%王奎建%郑振声%伍贵富
熊豔%廖曉星%囉景雲%陳國偉%何小洪%謝彊%方典鞦%馬虹%王奎建%鄭振聲%伍貴富
웅염%료효성%라경운%진국위%하소홍%사강%방전추%마홍%왕규건%정진성%오귀부
增强型体外反搏%动脉粥样硬化%内皮%血管舒张
增彊型體外反搏%動脈粥樣硬化%內皮%血管舒張
증강형체외반박%동맥죽양경화%내피%혈관서장
Enhanced external counterpulsation(EECP)%Atherosclerosis%Endothelium%Vasorelaxation
目的 探讨长期体外反搏对动脉粥样硬化猪内皮依赖和非内皮依赖血管舒张功能的影响.方法 猪龄20 d雄性乳猪18头随机分为正常饲养组(n=6)、高脂饲养组(n=6)及高脂饲养+体外反搏组(n=6).后两组通过高脂饲养复制动脉粥样硬化猪疾病模型,对高脂饲养+体外反搏组进行为时36 h的长期体外反搏治疗,取各组动物颈动脉血管环,测定离体颈动脉血管环对不同浓度梯度乙酰胆碱和硝普钠的舒张反应,用单因素方差分析法比较三组动物血管舒张率的差异.结果 在乙酰胆碱10-8～10-5mol/L浓度范围内,高脂饲养组和高脂饲养+体外反搏组的血管舒张率较正常饲养组显著降低(P＜0.05);在10-7～10-5mol/L浓度范围内,高脂饲养+体外反搏组的血管舒张率较高脂饲养组明显提高(P＜0.05).在硝普钠10-8～10-5mol/L浓度范围内,高脂饲养组和高脂饲养+体外反搏组的血管舒张率较正常饲养组显著降低(P＜0.05);在10-8～10-5mol/L浓度范围内,高脂饲养+体外反搏组的血管舒张率较高脂饲养组明显提高(P＜0.05).结论 高脂饲养组和高脂饲养+体外反搏组内皮依赖血管舒张功能和非内皮依赖血管舒张功能较正常饲养组明显受损,长期体外反搏干预可以显著改善动脉粥样硬化猪内皮依赖和非内皮依赖血管舒张功能,这可能是长期体外反搏具有抗动脉粥样硬化作用的机制之一.
目的 探討長期體外反搏對動脈粥樣硬化豬內皮依賴和非內皮依賴血管舒張功能的影響.方法 豬齡20 d雄性乳豬18頭隨機分為正常飼養組(n=6)、高脂飼養組(n=6)及高脂飼養+體外反搏組(n=6).後兩組通過高脂飼養複製動脈粥樣硬化豬疾病模型,對高脂飼養+體外反搏組進行為時36 h的長期體外反搏治療,取各組動物頸動脈血管環,測定離體頸動脈血管環對不同濃度梯度乙酰膽堿和硝普鈉的舒張反應,用單因素方差分析法比較三組動物血管舒張率的差異.結果 在乙酰膽堿10-8～10-5mol/L濃度範圍內,高脂飼養組和高脂飼養+體外反搏組的血管舒張率較正常飼養組顯著降低(P＜0.05);在10-7～10-5mol/L濃度範圍內,高脂飼養+體外反搏組的血管舒張率較高脂飼養組明顯提高(P＜0.05).在硝普鈉10-8～10-5mol/L濃度範圍內,高脂飼養組和高脂飼養+體外反搏組的血管舒張率較正常飼養組顯著降低(P＜0.05);在10-8～10-5mol/L濃度範圍內,高脂飼養+體外反搏組的血管舒張率較高脂飼養組明顯提高(P＜0.05).結論 高脂飼養組和高脂飼養+體外反搏組內皮依賴血管舒張功能和非內皮依賴血管舒張功能較正常飼養組明顯受損,長期體外反搏榦預可以顯著改善動脈粥樣硬化豬內皮依賴和非內皮依賴血管舒張功能,這可能是長期體外反搏具有抗動脈粥樣硬化作用的機製之一.
목적 탐토장기체외반박대동맥죽양경화저내피의뢰화비내피의뢰혈관서장공능적영향.방법 저령20 d웅성유저18두수궤분위정상사양조(n=6)、고지사양조(n=6)급고지사양+체외반박조(n=6).후량조통과고지사양복제동맥죽양경화저질병모형,대고지사양+체외반박조진행위시36 h적장기체외반박치료,취각조동물경동맥혈관배,측정리체경동맥혈관배대불동농도제도을선담감화초보납적서장반응,용단인소방차분석법비교삼조동물혈관서장솔적차이.결과 재을선담감10-8～10-5mol/L농도범위내,고지사양조화고지사양+체외반박조적혈관서장솔교정상사양조현저강저(P＜0.05);재10-7～10-5mol/L농도범위내,고지사양+체외반박조적혈관서장솔교고지사양조명현제고(P＜0.05).재초보납10-8～10-5mol/L농도범위내,고지사양조화고지사양+체외반박조적혈관서장솔교정상사양조현저강저(P＜0.05);재10-8～10-5mol/L농도범위내,고지사양+체외반박조적혈관서장솔교고지사양조명현제고(P＜0.05).결론 고지사양조화고지사양+체외반박조내피의뢰혈관서장공능화비내피의뢰혈관서장공능교정상사양조명현수손,장기체외반박간예가이현저개선동맥죽양경화저내피의뢰화비내피의뢰혈관서장공능,저가능시장기체외반박구유항동맥죽양경화작용적궤제지일.
Objective To explore the effect of long-term enhanced external counterpulsation(EECP)on endothelium-dependent and endothelium-independent vasorelaxation in the carotid arteries of atherosclerotic piss. Method Totally 18 20-day-old male infant pigs were randomly divided into 3 groups according to feeding given: the normal[control group(n=6),the hypercholesterolemic control group(n=6)and the hypereholesterolemic +EECP group(n=6).Porcine model of hypercholesterolemia was made by feeding high-cholesterol diet.After EECP for 36 hours in the hypercholesterolemic+EECP group(n=6),carotid arterial rings were harvested from all animals and their vaso-relaxation response to different dose of Acetylchofine(Ach)and Sodium nitroprusside (SNP)were detected,respectively.Results As the dose of Ach varying between 10-8 mol/L and 10-5mol/L, endothelium-dependent vasorelaxation ratio of hypereholesterolemic piss with or without EECP treatment was significantly lower than that of the normal control group(P＜0.05),however,endothehum-dependent vasorelax- ation ratio in pigs with EECP treatment was obviously higher compared with hypereholesterolemic pigs without EECP treatment(P＜0.05)as the Ach ranged from 10-7 mol/L to 10-5mol/L.Similarly,as the concentration of SNP ranged fiun 10-8 mol/L to 10-5 mol/L.endothelium-independent vasorelaxafion ratio of both the hypercholesterolemic control group and the hypercholesterolemic+EECP group were significantly lower than that of the normal control group(P＜0.05),and end othelium-independent vasorelaxation ratio of the hypercholesterolemic+EECP group was significantly higher than that of the hypercholesterolemic control group (P＜0.05).Condusions Long-term EECP improves the impaired endothelium-dependent and endothelium independent vasorelaxalion function resulting from atherosclerosis.
