中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2011年
1期
53-55
,共3页
王少华%黄斌%万永明%党红星%许峰%匡风梧
王少華%黃斌%萬永明%黨紅星%許峰%劻風梧
왕소화%황빈%만영명%당홍성%허봉%광풍오
急性呼吸窘迫综合征%NO供体%核因子-κB%细胞因子
急性呼吸窘迫綜閤徵%NO供體%覈因子-κB%細胞因子
급성호흡군박종합정%NO공체%핵인자-κB%세포인자
Acute respiratory distress syndrome%Neb-SNP%Nuclear factor-kappa B%Cytokine
目的 探讨雾化吸入NO供体对急性呼吸窘迫综合征炎症反应的干预作用机制.方法 健康出生后3～5 d的新生绪45只随机分成5组,A组为对照组:气管插管,接呼吸机;B组为NS组:模型建立后,经呼吸机管道雾化吸入生理盐水30min;C组为模型建立后,经呼吸机管道雾化吸入Neb-SNP(1 mg/ml,0.9%NaCl配制)30 min;D组为模型建立后,经呼吸机管道雾化吸入Neb-SNP(5 mg/ml)30min;E组为模型建立后,,经呼吸机管道雾化吸入Neb-SNP(10mg/ml)30min.各组分30、60、120 min3个时间点各3只测定肺组织核因子(NF)-κB的表达及血清细胞因子TNF-α、IL-12、IL-10的浓度.结果 治疗组肺组织NF-κB表达显著降低(P＜0.05),血清TNF-α、IL-12浓度显著降低(P＜0.05),IL-10浓度明显增高(P＜0.05);Neb-SNP雾化浓度增加,NF-κB表达下降,炎症因子TNF-α、IL-12浓度下降,IL-10浓度升高[C组与D组差异有显著性(P＜0.05),D组与E组差异无显著性(P＞0.05)].结论 Neb-SNP通过下调NF-κB的活性,抑制炎症因子的表达从而减轻急性呼吸窘迫综合征的肺损伤.
目的 探討霧化吸入NO供體對急性呼吸窘迫綜閤徵炎癥反應的榦預作用機製.方法 健康齣生後3～5 d的新生緒45隻隨機分成5組,A組為對照組:氣管插管,接呼吸機;B組為NS組:模型建立後,經呼吸機管道霧化吸入生理鹽水30min;C組為模型建立後,經呼吸機管道霧化吸入Neb-SNP(1 mg/ml,0.9%NaCl配製)30 min;D組為模型建立後,經呼吸機管道霧化吸入Neb-SNP(5 mg/ml)30min;E組為模型建立後,,經呼吸機管道霧化吸入Neb-SNP(10mg/ml)30min.各組分30、60、120 min3箇時間點各3隻測定肺組織覈因子(NF)-κB的錶達及血清細胞因子TNF-α、IL-12、IL-10的濃度.結果 治療組肺組織NF-κB錶達顯著降低(P＜0.05),血清TNF-α、IL-12濃度顯著降低(P＜0.05),IL-10濃度明顯增高(P＜0.05);Neb-SNP霧化濃度增加,NF-κB錶達下降,炎癥因子TNF-α、IL-12濃度下降,IL-10濃度升高[C組與D組差異有顯著性(P＜0.05),D組與E組差異無顯著性(P＞0.05)].結論 Neb-SNP通過下調NF-κB的活性,抑製炎癥因子的錶達從而減輕急性呼吸窘迫綜閤徵的肺損傷.
목적 탐토무화흡입NO공체대급성호흡군박종합정염증반응적간예작용궤제.방법 건강출생후3～5 d적신생서45지수궤분성5조,A조위대조조:기관삽관,접호흡궤;B조위NS조:모형건립후,경호흡궤관도무화흡입생리염수30min;C조위모형건립후,경호흡궤관도무화흡입Neb-SNP(1 mg/ml,0.9%NaCl배제)30 min;D조위모형건립후,경호흡궤관도무화흡입Neb-SNP(5 mg/ml)30min;E조위모형건립후,,경호흡궤관도무화흡입Neb-SNP(10mg/ml)30min.각조분30、60、120 min3개시간점각3지측정폐조직핵인자(NF)-κB적표체급혈청세포인자TNF-α、IL-12、IL-10적농도.결과 치료조폐조직NF-κB표체현저강저(P＜0.05),혈청TNF-α、IL-12농도현저강저(P＜0.05),IL-10농도명현증고(P＜0.05);Neb-SNP무화농도증가,NF-κB표체하강,염증인자TNF-α、IL-12농도하강,IL-10농도승고[C조여D조차이유현저성(P＜0.05),D조여E조차이무현저성(P＞0.05)].결론 Neb-SNP통과하조NF-κB적활성,억제염증인자적표체종이감경급성호흡군박종합정적폐손상.
Objective To investigate modulatory effects of Neb-SNP on inflammatory response and to explore the protection mechanisms of Neb-SNP in newborn piglets with ARDS. Methods Forty-five neonatal swines were randomly divided into five groups:group A (controlled group ,n = 9), group B (physiological saline group,n =9),group C (Neb-SNP 1 mg/ml,0. 9% NaCl, n = 9), group D (Neb-SNP 5 mg/ml,0. 9% NaCl, n = 9) and group E (Neb-SNP 10 mg/ml,0. 9% NaCl, n = 9). The pathological changes and activity of NF-κB in the lung tissue ,TNF-α ,IL-10 and IL-12 concentrations in serum at 30 minutes,60 minutes and 120 minutes after aerosol inhalation were observed. Results Activity of NF-κB and serum concentrations of TNF-α and IL-12 in the Neb-SNP treated group were lower than group B(P ＜0. 05) ,and serum IL-l0 concentration was obviously higher in the Neb-SNP group(P ＜0. 05). With an increase of Neb-SNP concentration,activity of NF-κB and serum concentrations of TNF-α and IL-12 were obviously increased, while serum concentrations of IL-10 was increased in group D and group E than that of group C (P ＜ 0. 05).Conclusion Inhalation of Neb-SNP reduced lung injury induced ARDS through lowering NF-κB activity and inhibiting expression of harmful inflammatory cytokines.
