中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2012年
4期
289-293
,共5页
张媛%赵延明%段甦%赵丽萍%张罗
張媛%趙延明%段甦%趙麗萍%張囉
장원%조연명%단소%조려평%장라
鼻炎,变应性,常年性%鼻炎,变应性,季节性%多态性,单核苷酸%免疫球蛋白E%受体,IgE
鼻炎,變應性,常年性%鼻炎,變應性,季節性%多態性,單覈苷痠%免疫毬蛋白E%受體,IgE
비염,변응성,상년성%비염,변응성,계절성%다태성,단핵감산%면역구단백E%수체,IgE
Rhinitis,allergic,perennial%Rhinitis,allergic,seasonal%Polymorphism,single nucleotide%Immunoglobulin E%Receptors,IgE
目的 探讨FCER1A基因单核苷酸多态性(single-nucleotide polymorphisms,SNP)与中国变应性鼻炎人群血清总IgE含量的相关性.方法 以378例确诊的变应性鼻炎患者为研究对象,血清总IgE检测使用UniCAP100检测系统,根据Hapmap网站公布的中国北京地区人群的FCER1A基因标签SNP位点信息及Haploview 4.1软件运行结果,选取覆盖FCER1A整个基因的代表性标签SNP位点共8个,MassARRAY实验平台完成各SNP位点的基因型检测,以SPSS 13.0软件进行统计学分析.结果 378例变应性鼻炎患者的血清总IgE含量在6.94 ～ 5000 kU/L之间,中位数为150 kU/L.Levene检验显示每个位点的不同基因型的患者血清总IgE含量的方差齐,ANOVA检验提示血清总IgE含量与各候选SNP位点基因型无关联性.结论 FCER1A基因SNP与本研究的中国北京地区变应性鼻炎患者的血清总IgE含量不相关.
目的 探討FCER1A基因單覈苷痠多態性(single-nucleotide polymorphisms,SNP)與中國變應性鼻炎人群血清總IgE含量的相關性.方法 以378例確診的變應性鼻炎患者為研究對象,血清總IgE檢測使用UniCAP100檢測繫統,根據Hapmap網站公佈的中國北京地區人群的FCER1A基因標籤SNP位點信息及Haploview 4.1軟件運行結果,選取覆蓋FCER1A整箇基因的代錶性標籤SNP位點共8箇,MassARRAY實驗平檯完成各SNP位點的基因型檢測,以SPSS 13.0軟件進行統計學分析.結果 378例變應性鼻炎患者的血清總IgE含量在6.94 ～ 5000 kU/L之間,中位數為150 kU/L.Levene檢驗顯示每箇位點的不同基因型的患者血清總IgE含量的方差齊,ANOVA檢驗提示血清總IgE含量與各候選SNP位點基因型無關聯性.結論 FCER1A基因SNP與本研究的中國北京地區變應性鼻炎患者的血清總IgE含量不相關.
목적 탐토FCER1A기인단핵감산다태성(single-nucleotide polymorphisms,SNP)여중국변응성비염인군혈청총IgE함량적상관성.방법 이378례학진적변응성비염환자위연구대상,혈청총IgE검측사용UniCAP100검측계통,근거Hapmap망참공포적중국북경지구인군적FCER1A기인표첨SNP위점신식급Haploview 4.1연건운행결과,선취복개FCER1A정개기인적대표성표첨SNP위점공8개,MassARRAY실험평태완성각SNP위점적기인형검측,이SPSS 13.0연건진행통계학분석.결과 378례변응성비염환자적혈청총IgE함량재6.94 ～ 5000 kU/L지간,중위수위150 kU/L.Levene검험현시매개위점적불동기인형적환자혈청총IgE함량적방차제,ANOVA검험제시혈청총IgE함량여각후선SNP위점기인형무관련성.결론 FCER1A기인SNP여본연구적중국북경지구변응성비염환자적혈청총IgE함량불상관.
Objective To investigate whether the single nucleotide polymorphisms (SNP) in the FCER1A gene are associated with serum total IgE level in Chinese allergic rhinitis (AR) cohort.Methods A total of 378 patients diagnosed as AR was included in this study.Serum total IgE level was detected by UniCAP100 testing system.A total of 8 reprehensive marker SNP which were in FCER1 A gene region were selected according to the Beijing people database from Hapmap website and the running results of Haploview 4.1 software.The individual genotyping was performed by MassARRAY platform.Results Three hundred and seventy-eight individuals (227 males and 151 females) with AR were prospectively recruited. The median of the total serum IgE measurements for the study population were 150 kU/L and ranged from 6.94 kU/L to 5000 kU/L.Levene tests showed the homogeneity of variance of total IgE level among different genotypes regarding each SNP locus.ANOVA tests demonstrated that none of the selected polymorphism loci in FCER1A was associated with total IgE level.Conclusion Present study failed to find an association between SNP in the FCER1A gene region and serum total IgE level in Chinese AR.
