CAJ | 학술논문

目的探讨低氧预适应对大鼠肝移植术后早期生长反应因子1(early growth response factor-1,EGR-1)的影响.方法采用门静脉灌注的大鼠自体肝移植模型:A组:低氧预适应大鼠自体肝移植组;B组:大鼠自体肝移植组;C组:正常大鼠对照组.比较大鼠自体肝移植术后肝脏组织病理学改变、肝组织中低氧诱导因子(hypoxic induce factor 1,HIF-1)、肿瘤坏死因子(tumor necrosis factor,TNF)的mRNA表达变化和早期生长反应因子1(EGR-1)的WB结果.结果 A组大鼠因术前低氧诱导致HIF-1 ENA的表达比B组和C组更明显,术后6h明显高于B组(t=9.601,df=10,2-tailed Sig=0.000,P<0.05);A组和B组的Egr-1蛋白表达量术后都增多,但A组明显低于B组.而B组肿瘤坏死因子RNA的RT-PCR表达比A组和C组更明显,其中术后6 h明显高于A组(t=-12.067,df=10,2-tailed Sig=0.000,P<0.05),与Esgr-1蛋白表达呈正相关.肝细胞病理结果示A组肝小叶结构损伤较B组轻,肝细胞轻微肿胀,肝组织无明显改变.结论低氧预适应后大鼠移植肝中EGR-1生成适度增加,与大鼠自体肝移植组相比明显减少,降低了TNF等炎性因子的产生从而减少肝脏的病理损害.
목적 탐토저양예괄응대대서간이식술후조기생장반응인자1(early growth response factor-1,EGR-1)적영향.방법 채용문정맥관주적대서자체간이식모형:A조:저양예괄응대서자체간이식조;B조:대서자체간이식조;C조:정상대서대조조.비교대서자체간이식술후간장조직병이학개변、간조직중저양유도인자(hypoxic induce factor 1,HIF-1)、종류배사인자(tumor necrosis factor,TNF)적mRNA표체변화화조기생장반응인자1(EGR-1)적WB결과.결과 A조대서인술전저양유도치HIF-1 ENA적표체비B조화C조경명현,술후6h명현고우B조(t=9.601,df=10,2-tailed Sig=0.000,P<0.05);A조화B조적Egr-1단백표체량술후도증다,단A조명현저우B조.이B조종류배사인자RNA적RT-PCR표체비A조화C조경명현,기중술후6 h명현고우A조(t=-12.067,df=10,2-tailed Sig=0.000,P<0.05),여Esgr-1단백표체정정상관.간세포병리결과시A조간소협결구손상교B조경,간세포경미종창,간조직무명현개변.결론 저양예괄응후대서이식간중EGR-1생성괄도증가,여대서자체간이식조상비명현감소,강저료TNF등염성인자적산생종이감소간장적병리손해.
Objective To investigate the effect of hypoxic preconditioning on hepatocytes early growth response factor 1 ( EGR-1 ) in a rat liver autotransplantation. Methods The rat portal vein perfusion model was established for donor liver autotransplantation. Rats were then divided into group A:hypoxic preconditioning was done before transplantation; group B: rats undergoing liver transplantation without preconditioning; group C: Normal control group of rats. Liver histopathological changes, the mRNA expression of HIF-1, TNF-1 and the WB results of EGR-1 were compared between groups. Results The expression of HIF-1 α RNA determined in group A was more obvious than in group B and group C. Six hours after surgery, the expression in group A was significantly higher than that in group B (t =9. 601, df= 10, 2-tailed Sig = 0. 000, P<0. 05 ) ; Egr-1 protein expression in group A and group B increased after surgery,with that in group A being significantly lower than that in group B. The RT-PCR expression of TNF-α RNA in group B compared with group A and group C was more obvious. Six hours after operation, the expression of TNF in group B was significantly higher than that in group A ( t = -12. 067, df = 10, 2-tailed Sig =0. 000, P<0. 05 ). The expression of Egr-1 was positively correlated with that of TNF. A liver cell pathology showed less severe injury in structure of hepatic lobule, mild swelling of liver cells, no significant changes in liver tissue. Conclusions Hypoxic preconditioning adaptation in rat liver transplantation generates modest increase in EGR-1, and reduces the production of TNF and other inflammatory factors.