CAJ | 학술논문

目的构建及鉴定过氧化物酶体增殖物激活受体(PPARγ)过表达肝癌细胞株,观察PPARγ基因对肝癌细胞功能的影响.方法通过构建Ad-PPARγ腺病毒,转染到Hep3B肝癌细胞株,逆转录-聚合酶链反应(RT-PCR)检测基因表达,Western blot对转染后细胞株PPARγ蛋白表达进行检测,并检测Ad-PPARγ腺病毒转染后Hep3B肝癌细胞的凋亡以及增殖.结果 RT-PCR检测发现基因得以成功转染,PPARγ基因表达增强,CCK-8检测发现转染后72h肝癌细胞增殖能力降低,转染后72h肝癌细胞的凋亡增加13.2%.结论 PPARγ基因的表达可抑制肝癌细胞的生长以及促进肝癌细胞的凋亡.
목적 구건급감정과양화물매체증식물격활수체(PPARγ)과표체간암세포주,관찰PPARγ기인대간암세포공능적영향.방법 통과구건Ad-PPARγ선병독,전염도Hep3B간암세포주,역전록-취합매련반응(RT-PCR)검측기인표체,Western blot대전염후세포주PPARγ단백표체진행검측,병검측Ad-PPARγ선병독전염후Hep3B간암세포적조망이급증식.결과 RT-PCR검측발현기인득이성공전염,PPARγ기인표체증강,CCK-8검측발현전염후72h간암세포증식능력강저,전염후72h간암세포적조망증가13.2%.결론 PPARγ기인적표체가억제간암세포적생장이급촉진간암세포적조망.
Objective To investigate the peroxisome proliferator-activated receptor (PPAR)γ's function in hepatic carcinoma cell on proliferation and apoptosis.Methods The hepatic carcinoma cell line Hep3B was used to setup a stable overexpression of PPARγ by adenovirus vector.The expression of PPARγ was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.Cell proliferation and apoptosis of overexpression cell line were measured by CCK-8 kit and flow cytometry assay kit for apoptosis separately.Results The expression vector was successfully transfected into Hep3B cell,and the expression of PPARγ was improved in overexpression Hep3B cell.The cell proliferation was decreased and the apoptosis rate was increased 13.2% in overexpression Hep3B cell after 72 h of transfection.Conclusion PPARγ inhibitor the cell proliferation and promote the apoptosis in hepatic carcinoma cell.