中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2009年
6期
504-507
,共4页
陈委%刘志雄%李萃%肖志强%刘运生%黄俊强%武晋廷
陳委%劉誌雄%李萃%肖誌彊%劉運生%黃俊彊%武晉廷
진위%류지웅%리췌%초지강%류운생%황준강%무진정
垂体腺瘤%固相pH梯度双向凝胶电泳%基质辅助激光解吸电离飞行时间质谱%蛋白质组
垂體腺瘤%固相pH梯度雙嚮凝膠電泳%基質輔助激光解吸電離飛行時間質譜%蛋白質組
수체선류%고상pH제도쌍향응효전영%기질보조격광해흡전리비행시간질보%단백질조
Pituitary adenomas%Immobilized pH gradient two- dimensional polyaerylamide gel electrophoresis%MALDI - TOF - MS%Proteome
目的 建立分辨率高和重复性好的侵袭性与非侵袭性垂体腺瘤的双向凝胶电泳图谱,并识别鉴定出其差异表达的蛋白质.方法 利用固相pH梯度双向凝胶电泳分离侵袭性与非侵袭性垂体腺瘤的总蛋白质,凝胶经银染显色后,运用ImageMaster 2D图像分析软件进行比较分析、识别差异表达的蛋白质,应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)及数据库搜索鉴定部分差异蛋白质点.结果 得到了分辨率较高、重复性好的侵袭性与非侵袭性垂体腺瘤的双向凝胶电泳图谱.比较分析图谱,初步对其中30个点进行了肽质指纹图分析,鉴定出一些与细胞周期调控、信号传导等有关的差异蛋白质.结论 建立了分辨率较高且重复性较好的侵袭性与非侵袭性垂体腺瘤的双向凝胶电泳图谱,并识别鉴定出一些侵袭性与非侵袭性垂体腺瘤的差异表达的蛋白质,为进一步筛选侵袭性垂体腺瘤的蛋白质表达数据库提供了基础.
目的 建立分辨率高和重複性好的侵襲性與非侵襲性垂體腺瘤的雙嚮凝膠電泳圖譜,併識彆鑒定齣其差異錶達的蛋白質.方法 利用固相pH梯度雙嚮凝膠電泳分離侵襲性與非侵襲性垂體腺瘤的總蛋白質,凝膠經銀染顯色後,運用ImageMaster 2D圖像分析軟件進行比較分析、識彆差異錶達的蛋白質,應用基質輔助激光解吸電離飛行時間質譜(MALDI-TOF-MS)及數據庫搜索鑒定部分差異蛋白質點.結果 得到瞭分辨率較高、重複性好的侵襲性與非侵襲性垂體腺瘤的雙嚮凝膠電泳圖譜.比較分析圖譜,初步對其中30箇點進行瞭肽質指紋圖分析,鑒定齣一些與細胞週期調控、信號傳導等有關的差異蛋白質.結論 建立瞭分辨率較高且重複性較好的侵襲性與非侵襲性垂體腺瘤的雙嚮凝膠電泳圖譜,併識彆鑒定齣一些侵襲性與非侵襲性垂體腺瘤的差異錶達的蛋白質,為進一步篩選侵襲性垂體腺瘤的蛋白質錶達數據庫提供瞭基礎.
목적 건립분변솔고화중복성호적침습성여비침습성수체선류적쌍향응효전영도보,병식별감정출기차이표체적단백질.방법 이용고상pH제도쌍향응효전영분리침습성여비침습성수체선류적총단백질,응효경은염현색후,운용ImageMaster 2D도상분석연건진행비교분석、식별차이표체적단백질,응용기질보조격광해흡전리비행시간질보(MALDI-TOF-MS)급수거고수색감정부분차이단백질점.결과 득도료분변솔교고、중복성호적침습성여비침습성수체선류적쌍향응효전영도보.비교분석도보,초보대기중30개점진행료태질지문도분석,감정출일사여세포주기조공、신호전도등유관적차이단백질.결론 건립료분변솔교고차중복성교호적침습성여비침습성수체선류적쌍향응효전영도보,병식별감정출일사침습성여비침습성수체선류적차이표체적단백질,위진일보사선침습성수체선류적단백질표체수거고제공료기출.
Objective To analyze the differential protein expression by high resolution and reproducibility two -dimensional electrophoresis profiles in the invasion and noninvasion pituitary adenomas. Methods The proteome from the invasion and noninvasion pituitary adenomas tissues were separated and identified by using a series of methods,including immobilized pH gradient two - dimensional polyaerylamide gel electrophoresis, silver staining, ImageMaster 2 DE analysis software, peptide mass fingerprint based on matrix -assisted laser desorption/ionization time of flight mass spectrometry ( MALDI - TOF - MS) and database searching. Results The good 2 DE pattern of the invasion and noninvasion pituitary adenomas tissue including resolution and reproducibility were obtained. The map of the invasion pituitary adenomas tissues was compared with the noninvasion pituitary adenomas. Thirty proteins were analyzed, some of which have the relationship of oncogenes, and others were the regulation proteins, which adjust the cell cycle and signal transduction. Conclusion These data suggest that we constructed a high resolution and reproducibility of two - dimensional electrophoresis profiles from the invasion and noninvasion pituitary adenomas and analyze the differential protein spots, which will be taken advantage to establishing the differential proteomic expression profiles. These data will be used for establishing the invasion pituitary adenomas proteome database.
