CAJ | 학술논문

目的研究海藻酸钠-聚赖氨酸-海藻酸钠包裹胰岛进行移植的效果.方法将Wistar大鼠的胰腺先行胶原酶胰管内注射消化,然后分离、纯化,所得胰岛经培养后制成微囊包膜的胰岛,微囊直径为0.4～0.5mm,每个微囊内包含1个胰岛.将微囊包膜及非包膜的胰岛移植至小鼠腹腔内,每个受体的移植量约1 000个胰岛.术后不用免疫抑制剂.结果微囊化的胰岛分别置含葡萄糖5.6mmol/L和16.7mmol/L的无血清TC199培养基中培养,其胰岛素的释放量,包膜组与非包膜组相比,差异不显著(P>0.05).微囊化胰岛移植组术后3天非空腹血糖从(22.0±0.51)mmol/L降至(7.8±0.48)mmol/L,维持正常血糖的时间平均为126天;单纯胰岛移植组术后移植物有功能存活不足8天.结论微囊化胰岛移植后可在不使用免疫抑制剂的情况下延长胰岛移植物的存活时间,逆转高血糖.
목적 연구해조산납-취뢰안산-해조산납포과이도진행이식적효과.방법 장Wistar대서적이선선행효원매이관내주사소화,연후분리、순화,소득이도경배양후제성미낭포막적이도,미낭직경위0.4～0.5mm,매개미낭내포함1개이도.장미낭포막급비포막적이도이식지소서복강내,매개수체적이식량약1 000개이도.술후불용면역억제제.결과 미낭화적이도분별치함포도당5.6mmol/L화16.7mmol/L적무혈청TC199배양기중배양,기이도소적석방량,포막조여비포막조상비,차이불현저(P>0.05).미낭화이도이식조술후3천비공복혈당종(22.0±0.51)mmol/L강지(7.8±0.48)mmol/L,유지정상혈당적시간평균위126천;단순이도이식조술후이식물유공능존활불족8천.결론 미낭화이도이식후가재불사용면역억제제적정황하연장이도이식물적존활시간,역전고혈당.
Objective To study the effects of alginate-polylysine-alginate(APA)microencapsulated islet in xenotransplantation.Methods Islets were isolated from Wistar rat pancreas by ductal rejection of collagenase,digested,and purified by using Ficoll gradient.After cultured,the islets were encapsulated in PAP semipermeable membranes.The microencapsule had a diameter ranging from 0.4 to 0.6mm with each microencapsule containing one islet. 1 000 encapsulated or non-encapsulated islets were intrapentoneally transplanted into the diabetic mice in the absence of immunosuppression.Results There was no signilicant difference in insulin Secretion between the encapsulated islets and non-encapsulated ones (P>0.05 ).Diabetes was reversed within 3 days after transplantation of encapsulated islets with the fasting plasma glucoselevels being droppedfrom(22.0±0.51)mmol/L to (7.8±0.48)mmol/L and maintaining a mean normoglycemia for 126 days. While the non-encapsulated islets only functioned less than 8 days posttransplantation Conclusion The encapsulated pancreatic islets, semipermeable membranes, can effectively prolong the xenograft survival without immunosuppression,and reverse hyperglycemia in diabetic mice.