CAJ | 학술논문

目的对2008年甘肃省新分离乙脑病毒的PrM和E基因区段进行序列测定和分析,明确新分离病毒的基因型别并对E基因序列的分子特征进行分析.方法对新分离乙脑病毒的PrM和E基因区段进行PCR扩增并测定序列.使用ClustalX2.09、MegAlign和Mega4软件对核苷酸和氨基酸序列进行分析并绘制系统发生树.结果系统进化分析结果显示6株病毒均为基因Ⅰ型乙脑病毒,并且与2001和2002年越南分离株、2004年日本分离株及2004年我国四川省分离株进化关系较近.新分离株与减毒活疫苗株SA14-14-2相比,E基因核苷酸同源性为87.5%～87.9%,氨基酸同源性为96.8%～97.2%.新分离株与疫苗株在E基因区段存在11处共同位点的氨基酸差异.结论 2008年甘肃省分离的乙脑病毒均为基因Ⅰ型乙脑病毒,新分离株E基因氨基酸序列与疫苗株相比有部分差异,但均不属于决定抗原性的关键位点.
목적 대2008년감숙성신분리을뇌병독적PrM화E기인구단진행서렬측정화분석,명학신분리병독적기인형별병대E기인서렬적분자특정진행분석.방법 대신분리을뇌병독적PrM화E기인구단진행PCR확증병측정서렬.사용ClustalX2.09、MegAlign화Mega4연건대핵감산화안기산서렬진행분석병회제계통발생수.결과 계통진화분석결과현시6주병독균위기인Ⅰ형을뇌병독,병차여2001화2002년월남분리주、2004년일본분리주급2004년아국사천성분리주진화관계교근.신분리주여감독활역묘주SA14-14-2상비,E기인핵감산동원성위87.5%～87.9%,안기산동원성위96.8%～97.2%.신분리주여역묘주재E기인구단존재11처공동위점적안기산차이.결론 2008년감숙성분리적을뇌병독균위기인Ⅰ형을뇌병독,신분리주E기인안기산서렬여역묘주상비유부분차이,단균불속우결정항원성적관건위점.
Objective To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene. Methods Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4. Results The six JEV strains were clustered in genotype Ⅰ. 87.5%-87.9% identity in nucleotide sequence and 96.8%--97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain. Conclusion Genotype Ⅰ JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.