CAJ | 학술논문

目的探讨妊娠期糖尿病(GDM)母鼠血清晚期糖基化终产物(AGE)在GDM致胎儿心脏发育异常中的作用.方法 54只SD孕鼠随机分为GDM组(30只)和对照组(24只).GDM组孕鼠给予2%链脲霉素40 mg/kg,对照组注射等量柠檬酸缓冲液.孕鼠于孕13、16、19 d随机剖宫取胎,观察胚胎心脏异常情况,检测母鼠血清AGE水平、免疫组化法检测AGE受体(RAGE)在子鼠心脏组织的表达情况.结果 GDM组各时间点子鼠心脏发育异常比例较对照组高(P<0.01).GDM组孕鼠用药3 d后血糖水平明显高于对照组(P<0.01),尿糖均阳性.GDM组AGE水平在孕13、16和19 d时[(5.72±0.68)AU/mg、(7.31±0.29)AU/mg和(7.77±0.39)AU/mg]较对照组[(4.45±0.27)AU/mg、(4.71±0.35)AU/mg和(4.37±0.44)AU/mg]显著升高(t=6.142、16.295和9.399,P均<0.01);孕鼠AGE水平与孕鼠血糖(r=0.717,P=0.000)及子鼠心脏畸形数(r=0.994,P=0.000)呈正相关.子鼠心脏RAGE表达与心脏畸形数呈正相关(r=0.638,P=0.004).结论孕鼠血清AGE升高可能是胚胎心脏发育异常的重要因素.
목적 탐토임신기당뇨병(GDM)모서혈청만기당기화종산물(AGE)재GDM치태인심장발육이상중적작용.방법 54지SD잉서수궤분위GDM조(30지)화대조조(24지).GDM조잉서급여2%련뇨매소40 mg/kg,대조조주사등량저몽산완충액.잉서우잉13、16、19 d수궤부궁취태,관찰배태심장이상정황,검측모서혈청AGE수평、면역조화법검측AGE수체(RAGE)재자서심장조직적표체정황.결과 GDM조각시간점자서심장발육이상비례교대조조고(P<0.01).GDM조잉서용약3 d후혈당수평명현고우대조조(P<0.01),뇨당균양성.GDM조AGE수평재잉13、16화19 d시[(5.72±0.68)AU/mg、(7.31±0.29)AU/mg화(7.77±0.39)AU/mg]교대조조[(4.45±0.27)AU/mg、(4.71±0.35)AU/mg화(4.37±0.44)AU/mg]현저승고(t=6.142、16.295화9.399,P균<0.01);잉서AGE수평여잉서혈당(r=0.717,P=0.000)급자서심장기형수(r=0.994,P=0.000)정정상관.자서심장RAGE표체여심장기형수정정상관(r=0.638,P=0.004).결론 잉서혈청AGE승고가능시배태심장발육이상적중요인소.
Objective To explore the effect of advanced glycation end product(AGE) in serum of maternal rats with gestational diabetes mellitus (GDM) on the heart development of their offsprings. Methods Fifty-four SD rats were randomly assigned into control group (n= 24) and GDM group (n=30) which were established by administration of streptozotocin intra-abdominally. On the gestational age of 13, 16, 19 days, all rats underwent hysterectomy to obtain the fetal heart tissues. Serum level of AGE and blood glucose level of maternal rats were tested. The expression of receptor AGE (RAGE) in fetal cardiac tissue were detected by immunohistoehemistry. Results The incidence of fetal heart defect in GDM group was significantly higher than the control group at each time point (P<0.01). Rats in GDM group had higher blood glucose level at each time point (P<0.01). The AGE levels of GDM group on gestational age of 13, 16 and 19 day [(5.72±0.68) U/mgpr, (7.31±0.29) U/mgpr and (7.77±0.39) U/mgpr] were significantly higher than those of the control group [(4.45±0.27) U/mgpr, (4.71±0. 35) U/mgpr and (4. 37±0. 44) U/rngpr] (t=6. 142, 16. 295, 0. 399,P<0. 01). The number of heart malformation in fetal rats (r=0.994,P=0. 000) and blood glucose (r=0. 717,P=0. 000) had the positive relationship with the maternal serum AGE level. The expression of RAGE in fetal heart was positively related with the number of fetal heart malformation (r= 0. 638,P= 0. 004). Conclusions The increased maternal serum AGE level in GDM rats may be an important factor in fetal heart dysplasia.