CAJ | 학술논문

除了马属动物外,种间妊娠的胚胎总在妊娠的一定时期流产.以往的研究多集中在宏观结构和解剖学差别的研究上,对于这种现象产生的分子机制却少有报道.为了从信号分子角度阐明种间妊娠维持失败的可能原因,我们通过免疫组化和明胶酶谱法比较了科间和同种妊娠部位整合素αvβ3、FAK、p-ERK、IGFⅡ、IGFBP-1、Shc、PTEN和MMP-2、-9表达的差异.科间妊娠是将金色中仓鼠(Mesocricetus auratus)的胚胎移植入假孕小鼠(Mus musculus)子宫并取妊娠D4、D8、D12的子宫为实验材料,相应时间同种小鼠胚胎移植小鼠妊娠后的子宫和假孕小鼠子宫分别为阳性和阴性对照.免疫组化结果表明: D8时,科间妊娠孕体integrin αvβ3、IGF-Ⅱ、IGFBP-1、PTEN、Shc、FAK的表达强度均与正常同种妊娠有差异.而妊娠D8时同种、科间妊娠子宫p-ERK的表达部位与表达量无明显差异.明胶酶谱显示,科间妊娠时MMP-2,-9的分泌模式与同种妊娠明显不同.这些结果表明,上述信号通路分子的差异表达有可能是造成科间妊娠时母胎界面异常的组织学表现的一个原因.
제료마속동물외,충간임신적배태총재임신적일정시기유산.이왕적연구다집중재굉관결구화해부학차별적연구상,대우저충현상산생적분자궤제각소유보도.위료종신호분자각도천명충간임신유지실패적가능원인,아문통과면역조화화명효매보법비교료과간화동충임신부위정합소αvβ3、FAK、p-ERK、IGFⅡ、IGFBP-1、Shc、PTEN화MMP-2、-9표체적차이.과간임신시장금색중창서(Mesocricetus auratus)적배태이식입가잉소서(Mus musculus)자궁병취임신D4、D8、D12적자궁위실험재료,상응시간동충소서배태이식소서임신후적자궁화가잉소서자궁분별위양성화음성대조.면역조화결과표명: D8시,과간임신잉체integrin αvβ3、IGF-Ⅱ、IGFBP-1、PTEN、Shc、FAK적표체강도균여정상동충임신유차이.이임신D8시동충、과간임신자궁p-ERK적표체부위여표체량무명현차이.명효매보현시,과간임신시MMP-2,-9적분비모식여동충임신명현불동.저사결과표명,상술신호통로분자적차이표체유가능시조성과간임신시모태계면이상적조직학표현적일개원인.
Interspecific embryos always abort at certain phase in pregnancy. The majority of previous studies focused on descriptions of macrostructure and anatomic differences and molecular mechanisms of these phenomena were rarely studied. In order to elucidate the possible roles of signal molecules in failure of interfamily pregnancy, we compared the expressive differences of integrin alpha v beta 3, FAK, p-ERK, IGFⅡ, IGFBP-1, Shc, PTEN and MMP-2, -9 between interfamily and intraspecific fetuses by immunohistochemistry and gelatin zymography. Interfamily pregnancy was achieved by transferring embryos of golden hamster Mesocricetus auratus into pseudopregnant mouse Mus musculus uteri. Interfamily pregnant fetuses of day 4, 8 and 12 formed the experimental group. The intraspecific pregnant mice uteri after implantation of mice embryos to the uteri of pseudopregnant mice at corresponding time provided positive and negative controls, respectively. The immunohistochemical results showed that the expressions of integrin αvβ3, IGF-Ⅱ, IGFBP-1, PTEN, Shc and FAK of interfamily fetuses on day 8 were all different to those of normal intraspecific pregnancy day 8. While the expressive localization and intensity of p-ERK both had no evidently different between interfamily and interspecific pregnancy on day 8. Gelatin zymography indicated obvious differences of secretive modes of MMP-2 and -9 between interfamily and intraspecific pregnancy. The results suggested that these signal molecules differentially expressed in uteri participate to the formation of abnormal histological exhibitions in maternal-fetal interface of interfamily pregnancy.