分子诊断与治疗杂志
分子診斷與治療雜誌
분자진단여치료잡지
JOURNAL OF MOLECULAR DIAGNOSIS AND THERAPY
2009年
2期
87-90
,共4页
周诚%黄维金%吴星%辜文洁%蓝海云%梁争论%李河民
週誠%黃維金%吳星%辜文潔%藍海雲%樑爭論%李河民
주성%황유금%오성%고문길%람해운%량쟁론%리하민
乙型肝炎病毒%核酸%血清学
乙型肝炎病毒%覈痠%血清學
을형간염병독%핵산%혈청학
Hepatitis B virus%Nucleic acid%Serology
目的 分析HBV DNA载量与血清学标志物的相关性.方法 应用乙型肝炎病毒核酸试剂定量检测468份献血员样本HBV DNA含量,并分别定性或定量检测HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc及抗-HBc IgM血清学标志物,计算不同病毒核酸水平样本中5个乙肝血清学标志物阳性率及HBsAg、抗-HBs水平的分布情况. 结果 HBV DNA阴性样本中HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc阳性率分别为12.8%、39.8%、0、21.1%、49.6%,而HBVDNA阳性样本中HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc血清学标志物阳性率分别为91.3%、0.7%、20.9%、65.0%、88.6%,血清学指标在不同HBVDNA水平的阳性率差异具有统计学意义(X2=197.4,P<0.01).HBVDNA载量与HBsAg水平成正相关性,与抗-HBs水平成负相关性. 结论低水平的HBsAg在不同HBV DNA载量水平的样本均有分布,HBsAg检测能力需要进一步提高,另外在我国人群中存在低乙型肝炎病毒核酸水平携带者,乙型肝炎病毒核酸可以弥补血清学检测试剂的不足.
目的 分析HBV DNA載量與血清學標誌物的相關性.方法 應用乙型肝炎病毒覈痠試劑定量檢測468份獻血員樣本HBV DNA含量,併分彆定性或定量檢測HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc及抗-HBc IgM血清學標誌物,計算不同病毒覈痠水平樣本中5箇乙肝血清學標誌物暘性率及HBsAg、抗-HBs水平的分佈情況. 結果 HBV DNA陰性樣本中HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc暘性率分彆為12.8%、39.8%、0、21.1%、49.6%,而HBVDNA暘性樣本中HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc血清學標誌物暘性率分彆為91.3%、0.7%、20.9%、65.0%、88.6%,血清學指標在不同HBVDNA水平的暘性率差異具有統計學意義(X2=197.4,P<0.01).HBVDNA載量與HBsAg水平成正相關性,與抗-HBs水平成負相關性. 結論低水平的HBsAg在不同HBV DNA載量水平的樣本均有分佈,HBsAg檢測能力需要進一步提高,另外在我國人群中存在低乙型肝炎病毒覈痠水平攜帶者,乙型肝炎病毒覈痠可以瀰補血清學檢測試劑的不足.
목적 분석HBV DNA재량여혈청학표지물적상관성.방법 응용을형간염병독핵산시제정량검측468빈헌혈원양본HBV DNA함량,병분별정성혹정량검측HBsAg、항-HBs、HBeAg、항-HBe、항-HBc급항-HBc IgM혈청학표지물,계산불동병독핵산수평양본중5개을간혈청학표지물양성솔급HBsAg、항-HBs수평적분포정황. 결과 HBV DNA음성양본중HBsAg、항-HBs、HBeAg、항-HBe、항-HBc양성솔분별위12.8%、39.8%、0、21.1%、49.6%,이HBVDNA양성양본중HBsAg、항-HBs、HBeAg、항-HBe、항-HBc혈청학표지물양성솔분별위91.3%、0.7%、20.9%、65.0%、88.6%,혈청학지표재불동HBVDNA수평적양성솔차이구유통계학의의(X2=197.4,P<0.01).HBVDNA재량여HBsAg수평성정상관성,여항-HBs수평성부상관성. 결론저수평적HBsAg재불동HBV DNA재량수평적양본균유분포,HBsAg검측능력수요진일보제고,령외재아국인군중존재저을형간염병독핵산수평휴대자,을형간염병독핵산가이미보혈청학검측시제적불족.
Objective To study the relationship between the copies of HBV and serologic makers. Mothods Detect HBV DNA and serum HBsAg, Anti-HBs, anti-HBc, HBeAg, anti-HBe and anti-HBc IgM of 468 samples. To c, omtpare the positive rate of different serum markers in different HBV DNA levels, and analyses the relationship between HBV DNA levels and quantitative HBsAg, anti-HBs levels. Results In HBV DNA-negative samples, the positive rote of HBsAg, anti-HBs, anti-HBc, HBeAg, anti-HBe is 12.8%, 11.3%, 0, 21.1%, 49.6% separately. In HBV DNA-positive samples, that is 9t.3%,0.7% ,20.9% ,65.0% ,88.6%. There are obviously significant difference among serological markers in different HBV DNA levels (χ2=197.4, P<0.01). The results show the positive correlation between HBV DNA levels and quantitative HBsAg and the negative correlation between HBV DNA levels and quantitative anti-HBs. The positive rate of HBeAg(χ2=39.6, P<0.01), anti-HBe(χ2=27.0, P<0.01), anti-HBc(χ2= 13.6, P<0.01) in HBV DNA-positive samples all are higher than that of in HBV DNA-negative samples. Conclusion The lower-level of HBsAg exits in HBV DNA-positive samples regardless of DNA levels. The detecting potency for HBsAg should be improved further. Moreover there are carriers with lower HBV DNA level in China. The combination of HBV serological assays with HBV DNA NAT will increase blood safety.
