农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2009年
4期
31-36
,共6页
徐岩%肖艳双%杜金霞%汪洪%郑伟%李营%庞实锋
徐巖%肖豔雙%杜金霞%汪洪%鄭偉%李營%龐實鋒
서암%초염쌍%두금하%왕홍%정위%리영%방실봉
Basic fibroblast growth factor (bFGF)%Plant bioreactor%Oil-body system%Agrobacterium tumefaciens%Brassica campestris L.
[Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Method] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed (Brassica campestris L.) by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows: explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.
