生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
6期
78-81
,共4页
五味子%色素%提取%稳定性
五味子%色素%提取%穩定性
오미자%색소%제취%은정성
Shizandra chinesis%pigment%extraction%stability
目的:研究五味子色素的提取工艺条件及色素稳定性.方法:在单因素实验基础上利用L_9(3~4)正交试验确定最佳提取工艺;以吸光度值的变化为指标,研究色素稳定性.结果:最佳提取工艺为:料液比1:25,乙醇浓度45%,浸提温度70℃,浸提时间40min;五味子色素光稳定性较好;在酸性、弱碱性条件下稳定,当pH≥12时出现颜色变化;对糖、氯化钠有一定稳定性;抗坏血酸和Na_2SO_3对色素影响较小;苯甲酸钠和H_2O_2对色素影响显著;金属离子Ca~(2+)、Mg~(2+)、Na~+对色素无明显影响,Fe~(2+)、Cu~(2+)、Al~(3+)对色素影响显著.
目的:研究五味子色素的提取工藝條件及色素穩定性.方法:在單因素實驗基礎上利用L_9(3~4)正交試驗確定最佳提取工藝;以吸光度值的變化為指標,研究色素穩定性.結果:最佳提取工藝為:料液比1:25,乙醇濃度45%,浸提溫度70℃,浸提時間40min;五味子色素光穩定性較好;在痠性、弱堿性條件下穩定,噹pH≥12時齣現顏色變化;對糖、氯化鈉有一定穩定性;抗壞血痠和Na_2SO_3對色素影響較小;苯甲痠鈉和H_2O_2對色素影響顯著;金屬離子Ca~(2+)、Mg~(2+)、Na~+對色素無明顯影響,Fe~(2+)、Cu~(2+)、Al~(3+)對色素影響顯著.
목적:연구오미자색소적제취공예조건급색소은정성.방법:재단인소실험기출상이용L_9(3~4)정교시험학정최가제취공예;이흡광도치적변화위지표,연구색소은정성.결과:최가제취공예위:료액비1:25,을순농도45%,침제온도70℃,침제시간40min;오미자색소광은정성교호;재산성、약감성조건하은정,당pH≥12시출현안색변화;대당、록화납유일정은정성;항배혈산화Na_2SO_3대색소영향교소;분갑산납화H_2O_2대색소영향현저;금속리자Ca~(2+)、Mg~(2+)、Na~+대색소무명현영향,Fe~(2+)、Cu~(2+)、Al~(3+)대색소영향현저.
Objective: To study the optimal extractive process and stability of the pigment extracted from Shizandra chinesis . Method: On the basis of single factor tests, the L_9 (3~4) orthogonal test was used in the optimization of technological parameters. The stability of pigment was discussed by the changes in absorbance. Result: The optimum extraction yield can be obtained by 45% ethanol heated up at 70℃ for 40 minutes with the material-solvent ratio of 1: 25(g/mL) . The pigment holds good resistance to light. Also, the pigment is stable in acidic and alkalescency conditions, but discoloration emerges when pH is equal to or above 12. The pigment is relatively stable with carbohydrates and sodium chloride. Ascorbic acid and sodium sulfite have no adverse effects on the pigment but sodium benzoate and H_2O_2 do. Metallic ions Ca~(2+), Mg~(2+) and Na~+ have no effect on the pigment, but Fe~(2+), Cu~(2+) and Al~(3+) have remarkable effects on it.
