时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
3期
564-565
,共2页
肾阳虚%血管升压素%水通道蛋白2
腎暘虛%血管升壓素%水通道蛋白2
신양허%혈관승압소%수통도단백2
Kidney-"yang" deficiency%Vasopressin%AQP_2
目的 研究肾阳虚状态下血管升压素-环磷酸腺苷-水通道蛋白2(AVP-cAMP-AQP_2)信号途径的功能变化,探讨肾主水的现代医学机制.方法 20只成年雄性SD大鼠分为对照组和模型组.主要采用放免法测定血AVP和肾髓质cAMP水平、免疫组化技术检测集合管AQP_2表达.结果 与对照组相比,模型组大鼠的尿量明显增加,血AVP和肾髓质cAMP水平下降,集合管上皮细胞AQP_2表达减少.结论 肾阳虚时AVP-cAMP-AQP_2信号途径功能下降.
目的 研究腎暘虛狀態下血管升壓素-環燐痠腺苷-水通道蛋白2(AVP-cAMP-AQP_2)信號途徑的功能變化,探討腎主水的現代醫學機製.方法 20隻成年雄性SD大鼠分為對照組和模型組.主要採用放免法測定血AVP和腎髓質cAMP水平、免疫組化技術檢測集閤管AQP_2錶達.結果 與對照組相比,模型組大鼠的尿量明顯增加,血AVP和腎髓質cAMP水平下降,集閤管上皮細胞AQP_2錶達減少.結論 腎暘虛時AVP-cAMP-AQP_2信號途徑功能下降.
목적 연구신양허상태하혈관승압소-배린산선감-수통도단백2(AVP-cAMP-AQP_2)신호도경적공능변화,탐토신주수적현대의학궤제.방법 20지성년웅성SD대서분위대조조화모형조.주요채용방면법측정혈AVP화신수질cAMP수평、면역조화기술검측집합관AQP_2표체.결과 여대조조상비,모형조대서적뇨량명현증가,혈AVP화신수질cAMP수평하강,집합관상피세포AQP_2표체감소.결론 신양허시AVP-cAMP-AQP_2신호도경공능하강.
Objective To detect the modern medical mechanism of Kidney-yang deficiency by researching the function of AVP-cAMP-AQP_2 in Kidney-"yang" Deficiency.Methods 20 male adult SD rats were randomly divided into control and model groups. Radioimmunoassay was employed to detect the AVP and cAMP levels, and immunohistochemistry was applied to measure AQP_2 expression in collecting ducts.Results Compared with those in the control, urine volume was decreased markedly, plasma AVP and cAMP levels in renal medullar tissue were decreased obviously, and AQP_2 expression was decreased significantly in model group.Conclusion The function of AVP-cAMP-AQP_2 pathway decreases in Kidney-"yang" deficiency.
