CAJ | 학술논문

目的:检测宫颈鳞癌肿瘤抑制基因1(KAI1)蛋白和mRNA的表达,结合临床病理资料探讨其与宫颈癌侵袭转移及复发和预后的关系.方法:样本包括正常对照宫颈组织20例,宫颈上皮内瘤变(CIN)组织20例,宫颈癌组织40例.采用免疫组织化学法检测3组间石蜡切片KAI1蛋白表达,采用反转录-多聚酶链反应(RT-PCR)方法检测对照组与宫颈癌组新鲜组织KAI1mRNA的表达.统计相关临床资料并随访其复发及预后情况,采用Cox比例风险模型及Logistic回归分析其与复发与预后的关系.结果:KAI1在正常宫颈组织、CIN及宫颈癌中基因及蛋白表达阳性率均呈进行性下降,组间比较差异显著(P<0.01).KAI1基因及蛋白表达均与宫颈鳞癌组织分化程度及淋巴转移有关,高分化组较低分化组阳性表达率显著降低(P<0.01),未转移组中的阳性率显著高于淋巴转移组(P<0.05).宫颈癌按临床分期不同期别越早蛋白表达率越高(P<0.05),基因表达率与临床期别无关.生存分析及Logistic回归分析显示组织学类型差,临床分期晚,有淋巴结转移及KAI1蛋白与RNA表达阳性患者具有高复发风险.临床分期、淋巴结转移及KAI1蛋白与RNA表达与否均为预后相关因素.结论:KAI1基因及蛋白表达强度与宫颈癌组织分化、临床分期及转移均有关,基因表达基本与蛋白表达同步变化,提示KAI1变化可能参与了宫颈癌发生发展,并且是宫颈癌复发与预后的相关因素.
목적:검측궁경린암종류억제기인1(KAI1)단백화mRNA적표체,결합림상병리자료탐토기여궁경암침습전이급복발화예후적관계.방법:양본포괄정상대조궁경조직20례,궁경상피내류변(CIN)조직20례,궁경암조직40례.채용면역조직화학법검측3조간석사절편KAI1단백표체,채용반전록-다취매련반응(RT-PCR)방법검측대조조여궁경암조신선조직KAI1mRNA적표체.통계상관림상자료병수방기복발급예후정황,채용Cox비례풍험모형급Logistic회귀분석기여복발여예후적관계.결과:KAI1재정상궁경조직、CIN급궁경암중기인급단백표체양성솔균정진행성하강,조간비교차이현저(P<0.01).KAI1기인급단백표체균여궁경린암조직분화정도급림파전이유관,고분화조교저분화조양성표체솔현저강저(P<0.01),미전이조중적양성솔현저고우림파전이조(P<0.05).궁경암안림상분기불동기별월조단백표체솔월고(P<0.05),기인표체솔여림상기별무관.생존분석급Logistic회귀분석현시조직학류형차,림상분기만,유림파결전이급KAI1단백여RNA표체양성환자구유고복발풍험.림상분기、림파결전이급KAI1단백여RNA표체여부균위예후상관인소.결론:KAI1기인급단백표체강도여궁경암조직분화、림상분기급전이균유관,기인표체기본여단백표체동보변화,제시KAI1변화가능삼여료궁경암발생발전,병차시궁경암복발여예후적상관인소.
AIM: To study the mRNA and protein expression of Kang ai1 (KAI1) tumor suppressor gene and to determine the relationship between KAI1 and invasiveness and metastasis of cervical cancer. METHODS: The expression of KAI1 metastasis suppressor was detected by immunohistochemistry in paraffin slides and by real-time quantitative polymerase chain reaction (RT-PCR) in fresh tissue. The samples included 20 cases of normal cervical tissues, 20 cases of cervical intraepithelial neoplasia (CIN) and 40 cases of cervical carcinoma. The results of the gene expression combined with the pathological and clinical data were also analyzed. RESULTS: The expression of KAI1 protein and mRNA was related to the tissue differentiation of cervix. The positive rates of KAI1 expression were the highest in the normal cervical tissue, the middle in CIN and the lowest in cervical carcinoma with significant difference among three groups (P<0.01). The expression of KAI1 protein was not related with the grade of CIN (P>0.05). However, both mRNA and protein expression of KAI1 were related to the differentiation and the clinical stages of cervical cancer (P<0.01) and also related to the metastasis of the cancer. The positive rates between the non-lymphatic metastasis and lymphatic metastasis (P<0.05) were significant different. Cox regression and logistic regression showed that the tissue differentiation, clinical stages, lymphatic metastasis and expression of KAI1 were all related factors with recurrence and prognosis of cervical cancer. CONCLUSION: The down-regulation of KAI1 tumor suppressor gene at both mRNA and protein levels is related to the differentiation, clinical stages and metastasis of cervical cancer, indicating that the expression of KAI1 is a prognostic factor for cervical cancer.