CAJ | 학술논문

利用68对SSR引物对91份粳稻品种进行了遗传多样性分析.研究结果共检测到293个等位基因,平均4.3个;平均多态信息含量(PIC)为0.313,变动范围为0.022～0.825.RM333和RM206的等位基因数最多,分别为14、10;且PIC也最高,分别为0.825、0.805.聚类和群体差异分析结果表明,东北三省水稻品种的遗传基础狭窄.黑龙江省和吉林省、黑龙江省和日本、吉林省和日本的水稻品种间遗传距离都很小,分别为0.083、0.084、0.090,而辽宁省与吉林省、黑龙江省的水稻品种遗传基础有一些差异.9个地理来源的品种聚类结果,可分为5个大类群,黑龙江省、吉林省、日本和韩国形成第Ⅰ类群;北京和辽宁省归为第Ⅱ类群;中国台湾、云南省、美国分别为第Ⅲ、第Ⅳ和第Ⅴ类群.东北三省是重要的粳稻生产基地,但遗传基础非常狭窄,要克服遗传脆弱性应从地理位置较远的国家或地区收集更丰富的遗传资源.
이용68대SSR인물대91빈갱도품충진행료유전다양성분석.연구결과공검측도293개등위기인,평균4.3개;평균다태신식함량(PIC)위0.313,변동범위위0.022～0.825.RM333화RM206적등위기인수최다,분별위14、10;차PIC야최고,분별위0.825、0.805.취류화군체차이분석결과표명,동북삼성수도품충적유전기출협착.흑룡강성화길림성、흑룡강성화일본、길림성화일본적수도품충간유전거리도흔소,분별위0.083、0.084、0.090,이요녕성여길림성、흑룡강성적수도품충유전기출유일사차이.9개지리래원적품충취류결과,가분위5개대류군,흑룡강성、길림성、일본화한국형성제Ⅰ류군;북경화요녕성귀위제Ⅱ류군;중국태만、운남성、미국분별위제Ⅲ、제Ⅳ화제Ⅴ류군.동북삼성시중요적갱도생산기지,단유전기출비상협착,요극복유전취약성응종지리위치교원적국가혹지구수집경봉부적유전자원.
Genetic diversity among 91 rice cultivars, which included 56 cuhivars from three provinces of Northeastern China(Liaoning, Jilin and Heilongjiang)and 35 cultivars from Beijing, Yunnan, Taiwan, Korea, Japan and USA, were analyzed using 68 SSR markers. A total of 293 alleles were produced, and the average number of alleles per marker locus was 4. 3. The PIC value per locus varied widely from 0. 022 to 0. 825 with an average value of 0. 313. RM333 and RM206 had the most alleles(14 and 10)and showed highest PIC(0. 825 and 0. 805). The unrooted neighbor-joining tree analysis and comparison of diversity parameters among provinces of Northeastern China and other groups revealed that rice cultivars from three Northeastern provinces had narrow genetic diversity. Cultivars from Heilongjiang and Jilin, Heilongjiang and Japan, Jilin and Japan had very near genetic distance, which were 0. 083, 0. 084 and 0. 090. Liaoning cultivars had some different genetic fundation from Heilongjiang and Jilin. Cluster analysis showed that the 9 regional groups could be clustered into five groups. The first group included Heilongjiang, Jilin, Japan and Korea, the second group included Beijing and Liaoning, and Taiwan, Yunnan, U S A each comprised the 3~(rd), the 4~(rd) and the 5~(rd) groups. These results collectively demonstrated that more diverse genetic resources from far geography position should be incorporated into rice cultivars in three provinces of Northeastern China in order to avoid the potential genetic vulnerability.