中国糖尿病杂志
中國糖尿病雜誌
중국당뇨병잡지
CHINESE JOURNAL OF DIABETES
2011年
1期
64-67
,共4页
王玉环%达婷%张静%吴娟娟%郑华东%闫丽丽
王玉環%達婷%張靜%吳娟娟%鄭華東%閆麗麗
왕옥배%체정%장정%오연연%정화동%염려려
游离脂肪酸%βTc3细胞%c-Jun氨基末端激酶%凋亡
遊離脂肪痠%βTc3細胞%c-Jun氨基末耑激酶%凋亡
유리지방산%βTc3세포%c-Jun안기말단격매%조망
Free fatty acid%βTc3 cells% c-Jun amino-terminal kinase% Apoptosis
目的 探讨c-Jun氨基末端激酶(JNK)抑制剂SP600125在游离脂肪酸(FFA)诱导的胰岛β细胞凋亡中的作用.方法 采用MTT法观察FFA对小鼠βTc3细胞生长的抑制作用,流式细胞术观察细胞凋亡率,免疫细胞化学和Western blot法检测p-JNK、p-c-Jun在FFA作用下及SP600125阻断JNK信号通路情况下的表达.结果 FFA可诱导体外生长的βTc3细胞凋亡,与对照组比较明显增加(P＜0.05).FFA诱导βTc3细胞凋亡过程中,p-JNK和p-c-Jun蛋白显著增加(P＜0.05);用SP600125.预处理βTc3细胞后,p-JNK和p-c-Jun蛋白含量较未处理组明显减少(P＜0.05).结论 FFA可诱导小鼠βTc3细胞凋亡,凋亡过程通过JNK途径实现.
目的 探討c-Jun氨基末耑激酶(JNK)抑製劑SP600125在遊離脂肪痠(FFA)誘導的胰島β細胞凋亡中的作用.方法 採用MTT法觀察FFA對小鼠βTc3細胞生長的抑製作用,流式細胞術觀察細胞凋亡率,免疫細胞化學和Western blot法檢測p-JNK、p-c-Jun在FFA作用下及SP600125阻斷JNK信號通路情況下的錶達.結果 FFA可誘導體外生長的βTc3細胞凋亡,與對照組比較明顯增加(P＜0.05).FFA誘導βTc3細胞凋亡過程中,p-JNK和p-c-Jun蛋白顯著增加(P＜0.05);用SP600125.預處理βTc3細胞後,p-JNK和p-c-Jun蛋白含量較未處理組明顯減少(P＜0.05).結論 FFA可誘導小鼠βTc3細胞凋亡,凋亡過程通過JNK途徑實現.
목적 탐토c-Jun안기말단격매(JNK)억제제SP600125재유리지방산(FFA)유도적이도β세포조망중적작용.방법 채용MTT법관찰FFA대소서βTc3세포생장적억제작용,류식세포술관찰세포조망솔,면역세포화학화Western blot법검측p-JNK、p-c-Jun재FFA작용하급SP600125조단JNK신호통로정황하적표체.결과 FFA가유도체외생장적βTc3세포조망,여대조조비교명현증가(P＜0.05).FFA유도βTc3세포조망과정중,p-JNK화p-c-Jun단백현저증가(P＜0.05);용SP600125.예처리βTc3세포후,p-JNK화p-c-Jun단백함량교미처리조명현감소(P＜0.05).결론 FFA가유도소서βTc3세포조망,조망과정통과JNK도경실현.
Objective To investigate the anti-apoptotic effects of JNK inhibitor SP600125 on apoptosis in mouse βTc3 cells. Methods MTT assay was used to observe the inhibitory effects of FFA on generation of βTc3 cells .The apoptotic rate of the cells was detected by flow cytometry. The protein expressions of p-JNK, p-c-Jun were detected by immunocytochemistry and Western blot. Results The growth of the βTc3 cells were obviously inhibited by FFA in vitro, and significant apoptosis was present. FFA induced activation of p-JNK, p-c-Jun. As a JNK inhibitor, SP600125 suppressed FFA -induced activation of p-JNK and p-c-Jun. Conclusion FFA can inhibit the proliferation of mouse βTc3 cells and induce βTC3 cells apoptosis. The JNK signaling pathway is involved in the mechanism of FFA-induced apoptosis.
