中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2012年
2期
123-125
,共3页
胰腺肿瘤%细胞系,肿瘤%模型,动物%磁共振成像%小鼠
胰腺腫瘤%細胞繫,腫瘤%模型,動物%磁共振成像%小鼠
이선종류%세포계,종류%모형,동물%자공진성상%소서
Pancreatic neoplasms%Cell line,tumor%Models,animal%Magnetic resonance imageing%Mice
目的 研究裸鼠荷载多时间节点人胰腺癌细胞的能力,分析其MRI表现及皮下移植瘤MRI检出率.方法 将人胰腺癌SW1990细胞悬液在第1天、第8天、第15天分别注射于6只裸鼠左腋窝、右腋窝、右腹股沟皮下,制成分别荷3、2、1枚瘤裸鼠各2只.3周后行MRI平扫加增强扫描.然后剥取皮下肿块行病理学检查.结果 实验期间受试裸鼠全部存活,12枚肿瘤均可见,肿瘤体积与生长时间呈正相关.MRI平扫检出9枚肿瘤,增强扫描又增加检出1枚肿瘤,2枚不能检出.未检出的肿块位于右腹股沟皮下,生长时间最短,长径＜5mm.裸鼠皮下种植瘤具有类似人胰腺癌的MRI信号,有出血、坏死灶,但所有肿瘤边缘清晰,具“假包膜”征.光镜下12枚肿块均可见类似人胰腺癌的细胞.结论 裸鼠可以分期皮下种植3枚人胰腺癌细胞肿瘤,并且至少存活3周,常规3.0T MRI尚不能完全检出生长时间1周、长径＜5mm的早期肿瘤.
目的 研究裸鼠荷載多時間節點人胰腺癌細胞的能力,分析其MRI錶現及皮下移植瘤MRI檢齣率.方法 將人胰腺癌SW1990細胞懸液在第1天、第8天、第15天分彆註射于6隻裸鼠左腋窩、右腋窩、右腹股溝皮下,製成分彆荷3、2、1枚瘤裸鼠各2隻.3週後行MRI平掃加增彊掃描.然後剝取皮下腫塊行病理學檢查.結果 實驗期間受試裸鼠全部存活,12枚腫瘤均可見,腫瘤體積與生長時間呈正相關.MRI平掃檢齣9枚腫瘤,增彊掃描又增加檢齣1枚腫瘤,2枚不能檢齣.未檢齣的腫塊位于右腹股溝皮下,生長時間最短,長徑＜5mm.裸鼠皮下種植瘤具有類似人胰腺癌的MRI信號,有齣血、壞死竈,但所有腫瘤邊緣清晰,具“假包膜”徵.光鏡下12枚腫塊均可見類似人胰腺癌的細胞.結論 裸鼠可以分期皮下種植3枚人胰腺癌細胞腫瘤,併且至少存活3週,常規3.0T MRI尚不能完全檢齣生長時間1週、長徑＜5mm的早期腫瘤.
목적 연구라서하재다시간절점인이선암세포적능력,분석기MRI표현급피하이식류MRI검출솔.방법 장인이선암SW1990세포현액재제1천、제8천、제15천분별주사우6지라서좌액와、우액와、우복고구피하,제성분별하3、2、1매류라서각2지.3주후행MRI평소가증강소묘.연후박취피하종괴행병이학검사.결과 실험기간수시라서전부존활,12매종류균가견,종류체적여생장시간정정상관.MRI평소검출9매종류,증강소묘우증가검출1매종류,2매불능검출.미검출적종괴위우우복고구피하,생장시간최단,장경＜5mm.라서피하충식류구유유사인이선암적MRI신호,유출혈、배사조,단소유종류변연청석,구“가포막”정.광경하12매종괴균가견유사인이선암적세포.결론 라서가이분기피하충식3매인이선암세포종류,병차지소존활3주,상규3.0T MRI상불능완전검출생장시간1주、장경＜5mm적조기종류.
Objective To establish nude mouse tumor models bearing human pancreatic adenocarcinoma SW1990 cells in multiple sites at different time-points and investigate the feasibiilty of multiple tumor-bearing in these models; then the findings and detection rate of 3.0T magnetic resonance image (MRI) in subcutaneous transplanted tumors was analyzed. Methods A total of 6 BALB/C nude mice were randomized into 3 groups (2 mice per group ).At the 1st,8th,15th day,the mice were injected subcutaneously with the suspension of SW1990 cells at left axilla and right axilla and right groin in sequence.Three weeks later,all the bearing-tumor mice were performed with MRI non-contrast enhanced scanning plus Gd-DTPA enhanced scan and the subcutaneous masses were subjected to pathological analysis.Results All the 6 nude mice were alive during the study and obvious mass was observed in every injected site.The tumor size was positively associated with the grwing time.There were 9 tumors which could be de.ted by noncontrast enhanced MRI scanning and one more tumor was detected by contrast enhanced scanning.2 tumors were not detected,the 2 tumors were located at subcutaneous of right groin,with the shortest growing time,and the major axis of the 2 un-detected tumors was less than 5mm.Despite the MRI findings of the transplanted masses similar to that of human pancreatic adenocarcinoma with bleeding,necrosis,they presented the characteristics of a clear rim,with "pseudocapeule" sign.All the 12 masses were similar with human pancreatic adenocarcinoma under light microscope.Conclusions It is feasible to transplant human pancreatic adenocarcinoma cell at three different subcutaneous sites (injected at three different points of time) in the nude mouse,with a minimal survival time of three weeks.However,routine 3.0T MRI cannot detect the early tumors (growing time within 1 week,major axis ＜5 mm).
