CAJ | 학술논문

目的探讨大鼠外周血CD68单核细胞比例和颊黏膜巨噬细胞浸润变化及二者相关性在口腔黏膜急性排斥反应中的作用和意义.方法 36只雌性Wistar大鼠按随机数字表随机分为移植组(15只)、创伤对照组(12只)及正常对照组(9只),建立口腔黏膜异种移植模型,分别于术后1、2、4周采用流式细胞术检测各组大鼠外周血CD68单核细胞百分数,免疫组化SP法检测颊黏膜巨噬细胞CD68的表达.结果移植组3个时点外周血CD68单核细胞百分数呈高低起伏的变化趋势,峰值出现在术后4周(43.1%),术后2周为最低值(10.4%);组织学观察显示术后1周巨噬细胞环形包绕密集浸润于移植物周围,术后2周转向浸润于移植物内部,术后4周随移植物坏死崩解巨噬细胞数量减少.术后1周移植组颊黏膜巨噬细胞计数达峰值580.0(195.5)个/高倍视野,随时间推移呈递减趋势[术后2周计数值为220.0(111.9)个/高倍视野,术后4周为173.0(97.7)个/高倍视野].结论大鼠口腔黏膜急性排斥反应中巨噬细胞可识别并参与排斥异种移植物,移植术后早期外周血单核细胞百分数可反映移植区颊黏膜巨噬细胞浸润情况.
목적 탐토대서외주혈CD68단핵세포비례화협점막거서세포침윤변화급이자상관성재구강점막급성배척반응중적작용화의의.방법 36지자성Wistar대서안수궤수자표수궤분위이식조(15지)、창상대조조(12지)급정상대조조(9지),건립구강점막이충이식모형,분별우술후1、2、4주채용류식세포술검측각조대서외주혈CD68단핵세포백분수,면역조화SP법검측협점막거서세포CD68적표체.결과 이식조3개시점외주혈CD68단핵세포백분수정고저기복적변화추세,봉치출현재술후4주(43.1%),술후2주위최저치(10.4%);조직학관찰현시술후1주거서세포배형포요밀집침윤우이식물주위,술후2주전향침윤우이식물내부,술후4주수이식물배사붕해거서세포수량감소.술후1주이식조협점막거서세포계수체봉치580.0(195.5)개/고배시야,수시간추이정체감추세[술후2주계수치위220.0(111.9)개/고배시야,술후4주위173.0(97.7)개/고배시야].결론 대서구강점막급성배척반응중거서세포가식별병삼여배척이충이식물,이식술후조기외주혈단핵세포백분수가반영이식구협점막거서세포침윤정황.
Objective To investigate the role of the dynamic process of peripheral blood CD68 mononuclear cells proportion and macrophages inflitration and possible correlation between them during acute rejection of rat oral mucosal xenotransplantation. Methods Thirty-six female wistar rats were divided into three groups randomly, including xenotransplantation group( n = 15 ), trauma control group( n = 12) and normal control group ( n = 9). The rat oral mucosa xenotransplantation model was established. The flow cytometry was used to evaluate the peripheral blood CD68 mononuclear cell and immunohistochemical assay performed to detect the macrophages infiltration one week (W1), two weeks (W2)and four weeks (W4) after xenotransplantation. Results The peripheral blood CD68 mononuclear cells percentage of each xenotransplantation group presented a rise and fall tendency at the three time points, and the peak value appeared at W4(43. 1% ), and the nadir at W2( 10.4% ). The macrophage counts achieved peak value in xenotransplantation group at W1 [ 580.0 (195.5) cell/high power field ], and then reduced with time. Conclusions The mononuclear cells and macrophage were capable of recognizing the xenograft and directly participated the acute rejection of rat oral mucosal xenotransplantation. The peripheral blood mononuclear cells percentage could reflect macrophage infiltrating condition at the early stage of the acute rejection.