中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2009年
10期
1083-1085
,共3页
王雷%牛新强%单保恩%何明%孟宪利%张冰%王士杰
王雷%牛新彊%單保恩%何明%孟憲利%張冰%王士傑
왕뢰%우신강%단보은%하명%맹헌리%장빙%왕사걸
食管鳞癌%PI3K%基质金属蛋白酶-7%免疫组织化学
食管鱗癌%PI3K%基質金屬蛋白酶-7%免疫組織化學
식관린암%PI3K%기질금속단백매-7%면역조직화학
Esophageal squamous cell carcinoma%PI3K%MMP-7%Immunohistochemistry
目的 研究PI3K和基质金属蛋白酶-7(MMP-7)在食管鳞癌组织(ESCC)中的表达及其与食管鳞癌细胞发生、发展与转移的关系以及两者之间的相关性.方法 应用免疫组织化学SP法检测24例正常食管黏膜、94例食管鳞癌组织中PI3K和MMP-7的表述情况.结果 食管癌组织中PI3K和MMP-7的阳性表达率分别为71.28%(67/94)和52.13%(49/94),与正常食管黏膜组织阳性表达率4.17%(1/24)和O%(0/24)相比,差异均有统计学意义(P均<0.01);PI3K蛋白的表达与食管癌细胞的分化程度、淋巴结转移、浸润深度及临床分期显著相关(P均<0.01);MMP-7表达率与淋巴结转移(P<0.05)、分化程度(P<0.05)、浸润深度(P<0.01)和临床分期(P<0.05)等因素有关;PI3K与MMP-7表达呈显著正相关(r=0.232,P=0.025).结论 PI3K、MMP-7表达与食管癌发生、发展及侵袭转移有关,可作为评价食管鳞癌生物学行为的指标.
目的 研究PI3K和基質金屬蛋白酶-7(MMP-7)在食管鱗癌組織(ESCC)中的錶達及其與食管鱗癌細胞髮生、髮展與轉移的關繫以及兩者之間的相關性.方法 應用免疫組織化學SP法檢測24例正常食管黏膜、94例食管鱗癌組織中PI3K和MMP-7的錶述情況.結果 食管癌組織中PI3K和MMP-7的暘性錶達率分彆為71.28%(67/94)和52.13%(49/94),與正常食管黏膜組織暘性錶達率4.17%(1/24)和O%(0/24)相比,差異均有統計學意義(P均<0.01);PI3K蛋白的錶達與食管癌細胞的分化程度、淋巴結轉移、浸潤深度及臨床分期顯著相關(P均<0.01);MMP-7錶達率與淋巴結轉移(P<0.05)、分化程度(P<0.05)、浸潤深度(P<0.01)和臨床分期(P<0.05)等因素有關;PI3K與MMP-7錶達呈顯著正相關(r=0.232,P=0.025).結論 PI3K、MMP-7錶達與食管癌髮生、髮展及侵襲轉移有關,可作為評價食管鱗癌生物學行為的指標.
목적 연구PI3K화기질금속단백매-7(MMP-7)재식관린암조직(ESCC)중적표체급기여식관린암세포발생、발전여전이적관계이급량자지간적상관성.방법 응용면역조직화학SP법검측24례정상식관점막、94례식관린암조직중PI3K화MMP-7적표술정황.결과 식관암조직중PI3K화MMP-7적양성표체솔분별위71.28%(67/94)화52.13%(49/94),여정상식관점막조직양성표체솔4.17%(1/24)화O%(0/24)상비,차이균유통계학의의(P균<0.01);PI3K단백적표체여식관암세포적분화정도、림파결전이、침윤심도급림상분기현저상관(P균<0.01);MMP-7표체솔여림파결전이(P<0.05)、분화정도(P<0.05)、침윤심도(P<0.01)화림상분기(P<0.05)등인소유관;PI3K여MMP-7표체정현저정상관(r=0.232,P=0.025).결론 PI3K、MMP-7표체여식관암발생、발전급침습전이유관,가작위평개식관린암생물학행위적지표.
Objective To study the expression of PI3K and MMP-7 in esophageal carcinoma and the rela-tionship between the expression of PI3K and MMP-7 and carcinogenesis and progression of esophageal carcinoma. Methods PI3K and MMP-7 expression were detected in 24 normal esophageal mucosa,94 primary tumor tissues with SP immunohistochemistal method. Results There were significant differences of PI3K and MMP-7 expressions between esophageal carcinoma and normal mucesa epithelium ( all P < 0.01 ) [71.28% (67/94) vs 4.17% ( 1/24 ) and 52.13% (49/94) vs 0% (0/24)]. There were significant correlations between PI3K expression and the degrees of differentiation,invasive depth,clinical staging and the metastasis of lymph node (all P <0.01 ). The positive ex-pression rate of MMP-7 had the relationship with metastasis of lymph node (P < 0.05 ), the degrees of differentiation ( P < 0.05 ) invasive depth ( P < 0.01 ), and clinical staging( P < 0.05 ) . There was a positive relationship between PI3K and MMP-7 expression in this study (r = 0. 232 ,P = 0.025). Conlusions PBK and MMP-7 play an impor-tant roles in carcinogenesis and progression of esophageal carcinoma and can be used as valuable biomarkers to eval-uate biological characteristics in esophageal squamous cell carcinoma.
