中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
7期
628-631
,共4页
梁燕%阚红卫%徐鹏夫%魏安祥%杨士友
樑燕%闞紅衛%徐鵬伕%魏安祥%楊士友
량연%감홍위%서붕부%위안상%양사우
帕金森病%抗帕丸%跳台实验%避暗实验%旋转实验
帕金森病%抗帕汍%跳檯實驗%避暗實驗%鏇轉實驗
파금삼병%항파환%도태실험%피암실험%선전실험
Parkinson disease%Kangpa bolus%Step-down test%Step-through test%Rotation test
目的 观察抗帕丸对帕金森病模型动物行为学和纹状体内多巴胺及代谢产物的影响.方法 建立小鼠肌肉震颤和僵直模型,记录抗帕丸能否拮抗其震颤僵直行为.利用小鼠跳台实验,避暗实验,观察抗帕丸能否改善其学习记忆行为.制作大鼠帕金森病模型,观察抗帕丸能否改变其旋转行为,并用ELISA法测定损伤侧纹状体内多巴胺(DA)及高香草酸(HVA)含量.结果 抗帕丸大、中剂量组小鼠肌肉震颤持续时间[(548.0±27.0)s,590.9±28.7)s]较模型组[(723.1±79.3)8]明显减少(P<0.01);抗帕丸大、中、小剂量组小鼠肌肉僵直持续时间[(2300.1±352.5)s,(2478.2 ±276.6)s,(2559.3±207.6)s]较模型组[(3194.5±251.7)s]明显减少(P<0.01);抗帕丸大、中剂量组小鼠跳台的错误次数[(1.60±0.97)次,(1.80±0.63)次]较模型组[(3.10±0.74)次]明显减少(P<0.01);抗帕丸大、中剂量组小鼠避暗的错误次数[(0.80±0.79)次,(1.10±0.74)次]较模型组[(2.30±0.68)次]明显减少(P<0.01);抗帕丸大、中剂量组治疗3周后,帕金森病大鼠30 min内的旋转圈数[(286.5±12.1)次,(296.6±12.7)次]较模型组[(340.6±18.8)次]明显减少(P<0.01);抗帕丸大、中剂量组损伤侧纹状体内DA[(18.90±4.01,17.30±3.01)nmol/L]、HVA[(1.50±1.39,1.39±0.53)nmoL/L]的含量较模型组[DA(9.43±1.79)nmol/L,HVA(0.87±0.12)nmol/L]明显增高(P<0.01).结论 抗帕丸对帕金森病模型动物具有一定治疗作用.
目的 觀察抗帕汍對帕金森病模型動物行為學和紋狀體內多巴胺及代謝產物的影響.方法 建立小鼠肌肉震顫和僵直模型,記錄抗帕汍能否拮抗其震顫僵直行為.利用小鼠跳檯實驗,避暗實驗,觀察抗帕汍能否改善其學習記憶行為.製作大鼠帕金森病模型,觀察抗帕汍能否改變其鏇轉行為,併用ELISA法測定損傷側紋狀體內多巴胺(DA)及高香草痠(HVA)含量.結果 抗帕汍大、中劑量組小鼠肌肉震顫持續時間[(548.0±27.0)s,590.9±28.7)s]較模型組[(723.1±79.3)8]明顯減少(P<0.01);抗帕汍大、中、小劑量組小鼠肌肉僵直持續時間[(2300.1±352.5)s,(2478.2 ±276.6)s,(2559.3±207.6)s]較模型組[(3194.5±251.7)s]明顯減少(P<0.01);抗帕汍大、中劑量組小鼠跳檯的錯誤次數[(1.60±0.97)次,(1.80±0.63)次]較模型組[(3.10±0.74)次]明顯減少(P<0.01);抗帕汍大、中劑量組小鼠避暗的錯誤次數[(0.80±0.79)次,(1.10±0.74)次]較模型組[(2.30±0.68)次]明顯減少(P<0.01);抗帕汍大、中劑量組治療3週後,帕金森病大鼠30 min內的鏇轉圈數[(286.5±12.1)次,(296.6±12.7)次]較模型組[(340.6±18.8)次]明顯減少(P<0.01);抗帕汍大、中劑量組損傷側紋狀體內DA[(18.90±4.01,17.30±3.01)nmol/L]、HVA[(1.50±1.39,1.39±0.53)nmoL/L]的含量較模型組[DA(9.43±1.79)nmol/L,HVA(0.87±0.12)nmol/L]明顯增高(P<0.01).結論 抗帕汍對帕金森病模型動物具有一定治療作用.
목적 관찰항파환대파금삼병모형동물행위학화문상체내다파알급대사산물적영향.방법 건립소서기육진전화강직모형,기록항파환능부길항기진전강직행위.이용소서도태실험,피암실험,관찰항파환능부개선기학습기억행위.제작대서파금삼병모형,관찰항파환능부개변기선전행위,병용ELISA법측정손상측문상체내다파알(DA)급고향초산(HVA)함량.결과 항파환대、중제량조소서기육진전지속시간[(548.0±27.0)s,590.9±28.7)s]교모형조[(723.1±79.3)8]명현감소(P<0.01);항파환대、중、소제량조소서기육강직지속시간[(2300.1±352.5)s,(2478.2 ±276.6)s,(2559.3±207.6)s]교모형조[(3194.5±251.7)s]명현감소(P<0.01);항파환대、중제량조소서도태적착오차수[(1.60±0.97)차,(1.80±0.63)차]교모형조[(3.10±0.74)차]명현감소(P<0.01);항파환대、중제량조소서피암적착오차수[(0.80±0.79)차,(1.10±0.74)차]교모형조[(2.30±0.68)차]명현감소(P<0.01);항파환대、중제량조치료3주후,파금삼병대서30 min내적선전권수[(286.5±12.1)차,(296.6±12.7)차]교모형조[(340.6±18.8)차]명현감소(P<0.01);항파환대、중제량조손상측문상체내DA[(18.90±4.01,17.30±3.01)nmol/L]、HVA[(1.50±1.39,1.39±0.53)nmoL/L]적함량교모형조[DA(9.43±1.79)nmol/L,HVA(0.87±0.12)nmol/L]명현증고(P<0.01).결론 항파환대파금삼병모형동물구유일정치료작용.
Objective To investigate the effects of Kangpa bolus on behaviors, dopamine and its metabolites of striatum in animals with Parkinson' s disease (PD). Methods The mice models of muscle tremor and rigor were established to observe the antagonism of Kangpa bolus. Step-down and step-through tests were used to evaluate the effects of Kangpa bolus on learning and memory function in mice. The rat model of PD was established to observe the effects of Kangpa bolus on rotation behaviors. The contents of DA and homovanillic acid( HVA) in the injured side of striatum were detected by ELISA. Results Compared with model group(723. 1 ±79.3) s,the duration of tremor in mice shortened significantly (P < 0. 01) in Kangpa bolus high dose and middle dose group ((548.0±27.0)s,(590.9 ±28.7)s). Compared with model group(3194.5 ±251.7)s,the duration of rigor in mice shortened significantly(P<0.01) in Kangpa bolus all dose group((2300.1 ±352.5)s,(2478.2 ±276.6)s, (2559.3 ±207.6) s). In step-down test, compared with model group (3. 10 ±0.74), the number of errors decreased significantly(P<0.01) in Kangpa bolus high dose and middle dose group (1.60 ±0. 97,1. 80 ±0.63). In step-through test, compared with model group( 2.30 ± 0. 68), the number of errors decreased significantly (P < 0.01) in Kangpa bolus high dose and middle dose group(0.80 ±0.79,1.10 ±0.74). Compared with model group (340.6 ±18.8) , the number of rotations of PD rats in thirty minutes reduced significantly (P< 0.01) in Kangpa bolus high dose and middle dose group(286.5 ± 12.1,296.6 ± 12.7) after three weeks treatment. Compared with model group(9.43 ±1.79,0. 87 ±0.12) nmol/L,the contents of DA and HVA in the injured side of striatum increased significantly(P<0. 01 ) in Kangpa bolus high dose( 18. 9 ±4. 01,1. 50 ± 1. 39) nmol/L and middle dose group (17.3±3.01,1.39±0.53)nmol/L Conclusion Kangpa bolus has some therapeutic effects on the animals of PD.
