中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
12期
2148-2151
,共4页
王权%温玉刚%唐华美%严东旺%彭志海
王權%溫玉剛%唐華美%嚴東旺%彭誌海
왕권%온옥강%당화미%엄동왕%팽지해
胃癌%杂合缺失%17号染色体%微卫星位点
胃癌%雜閤缺失%17號染色體%微衛星位點
위암%잡합결실%17호염색체%미위성위점
Gastric carcinoma%Loss of heterozygosity%Chromosome 17%Microsatellites locus
目的 对胃癌17号染色体微卫星位点进行杂合缺失精细定位研究,以寻找新胃癌相关杂合缺失区域及可能存在的抑癌基因.方法 在17号染色体上筛选出13个微卫星位点,然后与48例胃癌患者的肿瘤组织及正常组织进行多重聚合酶链反应(PCR).产物在ABI Prism 3730自动荧光测序仪进行毛细管电泳,以Genemapper3.2对电泳结果以进行杂合缺失分析.使用Fisher's精确检验对杂合缺失与临床病例资料进行分析.结果 17号染色体具有较高的杂合缺失现象(31％),D17S2196、D17S808和D17S1853位点没有有效数据.其中以D17S796位点杂合缺失率最高为48％( 10/21),D17S956位点杂合缺失率最低为20％ (6/30)；结合临床病例资料发现D17S956、D17S805位点与pTNM分期相关,D17S831、D17S921位点与分化相关；通过杂合缺失研究在17号染色体上发现3个候选抑癌基因可能存在的区域D17S1857-D17S805、D17S930-D17S1877、D17S1857-D17S805.结论 通过杂合缺失精细定位分析提示17号染色体上发现3个可能存在胃癌抑癌基因的区域.
目的 對胃癌17號染色體微衛星位點進行雜閤缺失精細定位研究,以尋找新胃癌相關雜閤缺失區域及可能存在的抑癌基因.方法 在17號染色體上篩選齣13箇微衛星位點,然後與48例胃癌患者的腫瘤組織及正常組織進行多重聚閤酶鏈反應(PCR).產物在ABI Prism 3730自動熒光測序儀進行毛細管電泳,以Genemapper3.2對電泳結果以進行雜閤缺失分析.使用Fisher's精確檢驗對雜閤缺失與臨床病例資料進行分析.結果 17號染色體具有較高的雜閤缺失現象(31％),D17S2196、D17S808和D17S1853位點沒有有效數據.其中以D17S796位點雜閤缺失率最高為48％( 10/21),D17S956位點雜閤缺失率最低為20％ (6/30)；結閤臨床病例資料髮現D17S956、D17S805位點與pTNM分期相關,D17S831、D17S921位點與分化相關；通過雜閤缺失研究在17號染色體上髮現3箇候選抑癌基因可能存在的區域D17S1857-D17S805、D17S930-D17S1877、D17S1857-D17S805.結論 通過雜閤缺失精細定位分析提示17號染色體上髮現3箇可能存在胃癌抑癌基因的區域.
목적 대위암17호염색체미위성위점진행잡합결실정세정위연구,이심조신위암상관잡합결실구역급가능존재적억암기인.방법 재17호염색체상사선출13개미위성위점,연후여48례위암환자적종류조직급정상조직진행다중취합매련반응(PCR).산물재ABI Prism 3730자동형광측서의진행모세관전영,이Genemapper3.2대전영결과이진행잡합결실분석.사용Fisher's정학검험대잡합결실여림상병례자료진행분석.결과 17호염색체구유교고적잡합결실현상(31％),D17S2196、D17S808화D17S1853위점몰유유효수거.기중이D17S796위점잡합결실솔최고위48％( 10/21),D17S956위점잡합결실솔최저위20％ (6/30)；결합림상병례자료발현D17S956、D17S805위점여pTNM분기상관,D17S831、D17S921위점여분화상관；통과잡합결실연구재17호염색체상발현3개후선억암기인가능존재적구역D17S1857-D17S805、D17S930-D17S1877、D17S1857-D17S805.결론 통과잡합결실정세정위분석제시17호염색체상발현3개가능존재위암억암기인적구역.
Objective To explore precise deleted regions on chromosome 17 and screen the candidate tumor suppressor genes (TSGs) related to gastric carcinoma.Methods Thirteen microsatellite loci on chromosome 17 were chosen.These polymorphic microsatellite markers in 48 cases of gastric cancer were analyzed by using polymerase chain reaction (PCR).PCR products were electrophoresed on an ABI 3730 DNA sequencer.Genemapper 3.2 software was used for loss of heterozygosity (LOH) scanning and analysis.Comparison between LOH frequency and clinicopathological factors was performed by fisher' s exact test.Results Chromosome 17 exhibited higher LOH frequency in gastric carcinoma (31％).There were no valid data on loci of D17S2196,D17S808 and D17S1853.The highest LOH frequency of 48％ (10/21) was seen at D17S796,and the lowest of 20％ (6/30) at D17S956,respectively.Associations between LOH and clinical information revealed that D17S956 and D17S805 were associated with pTNM stage,and D17S831 and D17S921 with differentiation stage.Three refined regiom (D17S1857-D17S805,D17S930-D17S1877 andD17S1857-D17S805) were mapped as candidate regions for TSGs.Conclusion Through our refined deletion mapping,we have found three candidate regions on chromosome 17 in which TSGs may be located.
