CAJ | 학술논문

目的研究RGC-32(response gene to complement 32)在IgA肾病(IgAN)儿童及正常肾组织中的表达及其意义.方法用免疫组织化学方法观察IgAN儿童及正常肾组织中RGC-32蛋白的表达与分布,并与α平滑肌肌动蛋白(α-SMA)、转化生长因子β1(TGF-β1)的表达、IgAN肾组织病理损伤程度及临床相关指标进行统计学分析.结果 RGC-32蛋白在IgAN及正常肾组织的肾小管均明显表达,而在肾小球、肾小管问质及肾血管未见表达.RGC-32在正常肾组织、IgAN轻度、中度及重度损伤组中的阳性表达指数分别为(18.29±6.22)%、(23.90±9.65)%、(31.23±9.86)%和(34.52±10.63)%.RGC.32在IgAN儿童肾组织的阳性表达指数与肾小球评分、肾小管间质评分均呈正相关(r=0.385,0.347,P＜0.05);与α-SMA、TGF-β1表达亦呈正相关(r=0.594,0.521,P＜0.01);而与Scr、尿NAG/Cr、尿A1b/Cr、尿IgG/Cr、尿α1微球蛋白/Cr均无相关(r=0.117,-0.115,-0.138,-0.176,-0.028,P均＞0.05).结论首次发现RGC-32蛋白在IgAN儿童和正常肾组织中表达于肾小管,而在肾小球、肾小管间质及肾血管未见表达.RGC-32可能参与了IgAN患儿的肾小管间质损伤,尤其是TGF-β1诱导的肾小管上皮细胞.间充质转分化(EMT)过程.
목적 연구RGC-32(response gene to complement 32)재IgA신병(IgAN)인동급정상신조직중적표체급기의의.방법 용면역조직화학방법관찰IgAN인동급정상신조직중RGC-32단백적표체여분포,병여α평활기기동단백(α-SMA)、전화생장인자β1(TGF-β1)적표체、IgAN신조직병리손상정도급림상상관지표진행통계학분석.결과 RGC-32단백재IgAN급정상신조직적신소관균명현표체,이재신소구、신소관문질급신혈관미견표체.RGC-32재정상신조직、IgAN경도、중도급중도손상조중적양성표체지수분별위(18.29±6.22)%、(23.90±9.65)%、(31.23±9.86)%화(34.52±10.63)%.RGC.32재IgAN인동신조직적양성표체지수여신소구평분、신소관간질평분균정정상관(r=0.385,0.347,P＜0.05);여α-SMA、TGF-β1표체역정정상관(r=0.594,0.521,P＜0.01);이여Scr、뇨NAG/Cr、뇨A1b/Cr、뇨IgG/Cr、뇨α1미구단백/Cr균무상관(r=0.117,-0.115,-0.138,-0.176,-0.028,P균＞0.05).결론 수차발현RGC-32단백재IgAN인동화정상신조직중표체우신소관,이재신소구、신소관간질급신혈관미견표체.RGC-32가능삼여료IgAN환인적신소관간질손상,우기시TGF-β1유도적신소관상피세포.간충질전분화(EMT)과정.
Objective To examine the expression of response gene to complement 32 (RGC-32) in renal tissue of children with IgA nephropathy (IgAN), and to explore its significance. Methods The subjects were 45 children diagnosed as IgAN by renal biopsy. The expression of RGC-32, α-smooth muscle actin (α-SMA) and transforming growth factor β1 (TGF-β1) was examined by immunohistochemistry staining. The correlation of RGC-32 expression with α-SMA,TGF-β1, degree of renal pathological lesions and clinical index in IgAN was assessed by Spearman correlation analysis. Results RGC-32 protein located in renal tubular epithelial cells in normal and IgAN renal tissues. The positive expression index of RGC-32 in nomal group, IgAN mild group, moderate group and severe group was (18.29±6.22)%, (23.90±9.65)%, (31.23±9.86)%,and (34.52±10.63)% respectively. With more severity of renal pathological lesions, the expression of RGC-32 in IgAN was enhanced. The RGC-32 expression was positively correlated with the score of glomerulus and renal interstitium in children with IgAN (r=0.385, 0.347, P＜0.05), as well as α-SMA, TGF-β1 (r=0.594, 0.521, P＜0.01), but was not correlated with Scr, urinary NAG/Cr,Alb/Cr, IgG/Cr, and α1-M/Cr (r =0.117, -0.115, -0.138, -0.176, -0.028, all P ＞0.05).Conclusions RGC-32 protein locates in renal tubular epithelial cells in normal and IgAN renal tissues. RGC-32 may participate in the course of renal tubulointerstitial lesions in children with IgAN, especially in the course of epithelial-mesenchymal transition (EMT) induced by TGF-β1.