CAJ | 학술논문

目的了解江苏地区婴幼儿腹泻患者中诺如病毒的感染情况,明确该地区诺如病毒的主要基因型别和流行病学特征.方法收集2010年江苏省苏州市婴幼儿医院及南京市婴幼儿医院疑似病毒性腹泻粪便标本498例,采用荧光定量PCR方法检测粪便中诺如病毒RNA及其基因组别,并对部分阳性标本测序以明确基因型.结果 498份粪便标本中,GⅠ组诺如病毒阳性标本为2例,阳性率为0.4％,GⅡ组诺如病毒为190例,阳性率为38％,对本地区部分诺如病毒阳性标本进行测序,2份GⅠ标本中,一株为GⅠ1型,另一株为GⅠ3型；测序的23份GⅡ标本中21株为GⅡ4型,2株为GⅡ3型.结论诺如病毒是本省婴幼儿病毒性腹泻的重要病原体,以诺如病毒GⅡ4型为主,同时也存在其他型别的感染流行.
목적 료해강소지구영유인복사환자중낙여병독적감염정황,명학해지구낙여병독적주요기인형별화류행병학특정.방법 수집2010년강소성소주시영유인의원급남경시영유인의원의사병독성복사분편표본498례,채용형광정량PCR방법검측분편중낙여병독RNA급기기인조별,병대부분양성표본측서이명학기인형.결과 498빈분편표본중,GⅠ조낙여병독양성표본위2례,양성솔위0.4％,GⅡ조낙여병독위190례,양성솔위38％,대본지구부분낙여병독양성표본진행측서,2빈GⅠ표본중,일주위GⅠ1형,령일주위GⅠ3형；측서적23빈GⅡ표본중21주위GⅡ4형,2주위GⅡ3형.결론 낙여병독시본성영유인병독성복사적중요병원체,이낙여병독GⅡ4형위주,동시야존재기타형별적감염류행.
Objective To investigate norovirus infection status and indentify its epidemiological characteristics and genotype distribution in infantile viral diarrhea in Jiangsu.Methods Four hundred and ninety-eight fecal specimens of infantile virus diarrhea cases were collected from Suzhou Children's hospital and Nanjing Children's hospital in 2010.Norovirus genegroup were detected by real-time RT-PCR,and genetype were determined by sequence analysis.Results Among all fecal specimens,2 (0.4％) cases were positive for norovirus G Ⅰ,and 190 (38％) cases for G Ⅱ.Nucleotide sequence analysis revealed that in the 2 samples for G Ⅰ,one strain was G Ⅰ 1 and another was G Ⅰ 3.Twenty-one strains were belonged to G Ⅱ 4 and 2 strains were G Ⅱ 3 in the 23 samples for G Ⅱ.Conclusion As one of the most important pathogens causing infantile viral diarrhea in Jiangsu province,subtype G Ⅱ 4 was the main epidemic strain of norovirus,meanwhile other genotypes also existed.