中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
15期
1017-1021
,共5页
高增鑫%翁习生%邱贵兴%吴志宏%崔巍%邵柏%梅素萍%肖军%杨新宇
高增鑫%翁習生%邱貴興%吳誌宏%崔巍%邵柏%梅素萍%肖軍%楊新宇
고증흠%옹습생%구귀흥%오지굉%최외%소백%매소평%초군%양신우
细胞膜组织%糖皮质激素类%微循环%微粒
細胞膜組織%糖皮質激素類%微循環%微粒
세포막조직%당피질격소류%미순배%미립
Cell membrane structures%Glucocorticosteroids%Microcirculation%Microparticles
目的 探讨糖皮质激素应用与血浆细胞膜微粒(MPs)改变的相关性.方法 采集33例大剂量糖皮质激素应用史的患者(A组)及24例年龄、性别、种族匹配的健康志愿者(B组)的新鲜血;离心分离出乏血小板血浆;应用鼠抗人单克隆抗体PE-CD31、FITC-CD42b、FITC-CD45、FITC-CD51/61、PE-CD54、PEcy5-CD62E、FITC-CD105标定;流式细胞仪测定膜微粒的数量.结果 两组血浆细胞膜微粒的平均秩次CD31+MPs(24.1 vs 35.7,P=0.009)、CD45+MPs(25.5 vs 33.8,P=0.019)、CD51/61+MPs(25.6 vs 33.7,P=0.045)、CD31+CD42b+MPs(21.6 vs 39.3,P=0.000)、CD31+CD45+MPs(24.8 vs 34.8,P=0.009)差异具有统计学意义,糖皮质激素应用组明显低于健康对照组.结论 糖皮质激素应用可导致血浆细胞膜微粒明显减少;细胞膜微粒代谢失衡可能与微循环障碍的发病机制相关.
目的 探討糖皮質激素應用與血漿細胞膜微粒(MPs)改變的相關性.方法 採集33例大劑量糖皮質激素應用史的患者(A組)及24例年齡、性彆、種族匹配的健康誌願者(B組)的新鮮血;離心分離齣乏血小闆血漿;應用鼠抗人單剋隆抗體PE-CD31、FITC-CD42b、FITC-CD45、FITC-CD51/61、PE-CD54、PEcy5-CD62E、FITC-CD105標定;流式細胞儀測定膜微粒的數量.結果 兩組血漿細胞膜微粒的平均秩次CD31+MPs(24.1 vs 35.7,P=0.009)、CD45+MPs(25.5 vs 33.8,P=0.019)、CD51/61+MPs(25.6 vs 33.7,P=0.045)、CD31+CD42b+MPs(21.6 vs 39.3,P=0.000)、CD31+CD45+MPs(24.8 vs 34.8,P=0.009)差異具有統計學意義,糖皮質激素應用組明顯低于健康對照組.結論 糖皮質激素應用可導緻血漿細胞膜微粒明顯減少;細胞膜微粒代謝失衡可能與微循環障礙的髮病機製相關.
목적 탐토당피질격소응용여혈장세포막미립(MPs)개변적상관성.방법 채집33례대제량당피질격소응용사적환자(A조)급24례년령、성별、충족필배적건강지원자(B조)적신선혈;리심분리출핍혈소판혈장;응용서항인단극륭항체PE-CD31、FITC-CD42b、FITC-CD45、FITC-CD51/61、PE-CD54、PEcy5-CD62E、FITC-CD105표정;류식세포의측정막미립적수량.결과 량조혈장세포막미립적평균질차CD31+MPs(24.1 vs 35.7,P=0.009)、CD45+MPs(25.5 vs 33.8,P=0.019)、CD51/61+MPs(25.6 vs 33.7,P=0.045)、CD31+CD42b+MPs(21.6 vs 39.3,P=0.000)、CD31+CD45+MPs(24.8 vs 34.8,P=0.009)차이구유통계학의의,당피질격소응용조명현저우건강대조조.결론 당피질격소응용가도치혈장세포막미립명현감소;세포막미립대사실형가능여미순배장애적발병궤제상관.
Objective To explore the relationship between the alterations of circulating cell-derived microparticles(MPs)and large-dose glucocorticosteroid application.Methods Peripheral blood samples were collected from 33 patients with history of large-dose glucocorticosteroid application(glucocorticosteroid group)and 24 age-,sex-,and race-matched healthy controls(healthy control group).Platelet-poor plasma was obtained by centrifugation.Plasma microparticles were labeled with monoclonal antibodies of PE-conjugated mouse anti-human CD31,CD54,and FITC-conjugated mouse anti-human CD42b,CD45,CD51/61,CD105,and PE-Cy5-conjugated mouse anti-human CD62E.Cell-derived microparticles were measured by three-color flow cytometry.Results The mean ranks of CD31+MPs,CD45+MPs,CD51/61+MPs,CD31+CD42b+MPs,and CD31+CD45+MPs of the glucoconicosteroid group were:24.1,25.5,25.6,21.6,and 24.8 respectively,all significantly lower than those of the control group(35.7,33.8,33.7,39.3,and 34.8 respectively,P=0.009,0.019,0.045,0.000,and 0.009).Conclusion High-dose glucocorticosteroid application remarkably reduces plasma MPs that may be responsible for microcirculation disturbance.
