中国人民解放军军医大学学报
中國人民解放軍軍醫大學學報
중국인민해방군군의대학학보
JOURNAL OF MEDICAL COLLEGES OF PLA
2003年
3期
170-174
,共5页
牟娇%李晓冬%杨庆%贾凤岐%卫立辛%郭亚军%吴孟超
牟嬌%李曉鼕%楊慶%賈鳳岐%衛立辛%郭亞軍%吳孟超
모교%리효동%양경%가봉기%위립신%곽아군%오맹초
hTRT antisense gene%gene expression%inhibition of telomerase
Objective: To induce changes in biological character of human liver cancer cell line SMMC-7721 by blocking the expression of telomerase genes hTRT and to explore its value in cancer gene therapy. Methods: The vehicle for eukaryotic expression of antisense hTRT was constructed and then transfected into SMMC-7721 cells. The effects of antisense hTRT gene on telomerase activity, cancer cell growth and malignant phenotypes were analyzed. Results: The obtained transfectants that could express antisense hTRT gene stably showed marked decrease in telomerase activity; the shortening of telomere was obvious; cells presented contact growth inhibition; in nude mice transplantation, the rate of tumor induction dramatically decreased. Conclusion: Antisense hTRT gene expression can significantly inhibit telomerase activity of cancer cells and decrease malignant phenotypes in vitro and in vivo. Therefore, as a telomerase inhibitor, antisense hTRT gene may be a new pathway for cancer therapy.
